Sodium borohydride/chloranil-based assay for quantifying total flavonoids

Journal of Agricultural and Food Chemistry

Volumn 56, Issue 20, 2008, Pages 9337-9344

  He, Xiangjiu ^a   Liu, Dong ^a   Rui, Hai Liu ^a  

^a Department of Molecular Biology and Genetics   (United States)

Author keywords

Anthocyanins; Antioxidant; Diet and cancer; Flavonoids; Fruits; Phenolics; Phytochemicals; Vegetables

Indexed keywords

BORANE DERIVATIVE; CHLORANIL; FLAVONOID; PLANT EXTRACT;

ARTICLE; CHEMISTRY; EVALUATION; FRUIT; METHODOLOGY; SPECTROPHOTOMETRY; VEGETABLE;

BOROHYDRIDES; CHLORANIL; FLAVONOIDS; FRUIT; PLANT EXTRACTS; SPECTROPHOTOMETRY; VEGETABLES;

BRASSICA OLERACEA VAR. ITALICA; CAPSICUM ANNUUM VAR. ANNUUM; MALUS X DOMESTICA; VACCINIUM; VACCINIUM MACROCARPON;

EID: 55549117860     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf070954+     Document Type: Article

References (27)

1. Block, G.; Patterson, B.; Subar, A. Fruit, vegetables, and cancer prevention: a review of the epidemiological evidence. Nutr. Cancer 1992, 18, 1-29.
2. Willett, W. C. Balancing life-style and genomics research for disease prevention. Science 2002, 296, 695-698.
3. Liu, R. H. Potential synergy of phytochemicals in cancer prevention: mechanism of action. J. Nutr. 2004, 134, 3479S-3485S.
4. Liu, R. H. Health benefits of fruits and vegetables are from additive and synergistic combination of phytochemicals. Am. J. Clin. Nutr. 2003, 78, 517S-520S.
5. Hanasaki, Y.; Ogawa, S.; Fukui, S. The correlation between active oxygens scavenging and antioxidative effects of flavonoids. Free Radical Biol. Med. 1994, 16, 845-850.
6. Hertog, M. G.; Hollman, P. C. Potential health effects of the dietary flavonol quercetin. Eur. J. Clin. Nutr. 1996, 50, 63-71.
7. Hertog, M. G.; Feskens, E. J.; Hollman, P. C.; Katan, M. B.; Kromhout, D. Dietary flavonoids and cancer risk in the Zutphen Elderly Study. Nutr. Cancer 1994, 22, 175-184.
8. Hertog, M. G.; Feskens, E. J.; Hollman, P. C.; Katan, M. B.; Kromhout, D. Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study. Lancet 1993, 342, 1007-1011.
9. Hollman, P. C. H.; Arts, I. C. W. Flavonols, flavones and flavanols - nature, occurrence and dietary burden. J. Sci. Food Agric. 2000, 80, 1081-1093.
10. Singleton, V. L.; Rossi, J. A., Jr. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic. 1965, 16, 144-158.
11. Singleton, V. L.; Orthofer, R.; Lamuela-Raventos, R. M. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 1999, 299, 152178.
12. Vaya, J.; Mahmood, S. Flavonoid content in leaf extracts of the fig Ficus carica L.), carob (Ceratonia siliqua L.) and pistachio (Pistacia lentiscus L.). BioFactors 2006, 28, 169-175.
13. Bloor, S. J. Overview of methods for analysis and identification of flavonoids. Methods Enzymol. 2001, 335, 3-14.
14. Viswanathan, P.; Sriram, V.; Yogeeswaran, G. Sensitive spectrophotometric assay for 3-hydroxy-substituted flavonoids, based on their binding with molybdenum, antimony, or bismuth. J. Agric. Food Chem. 2000, 48, 2802-2806.
15. Vinson, J. A.; Hao, Y.; Su, X.; Zubik, L.; Bose, P. Phenol antioxidant quantity and quality in foods: fruits. J. Agric. Food Chem. 2001, 49, 5315-5321.
16. Dewanto, V.; Wu, X. Z.; Liu, R. H. Processed sweet corn has higher antioxidant activity. J. Agric. Food Chem. 2002, 50, 4959-4964.
17. Chu, Y. F.; Sun, J.; Wu, X.; Liu, R. H. Antioxidant and antiproliferative activities of vegetables. J. Agric. Food Chem. 2002, 50, 6910-6916.
18. Sun, J.; Liu, R. H. Cranberry phytochemical extracts induce cell cycle arrest and apoptosis in human MCF-7 breast cancer cells. Cancer Lett. 2006, 241, 124-134.
19. Liu, R. H.; Liu, J.; Chen, B. Apples prevent mammary tumors in rats. J. Agric. Food Chem. 2005, 53, 2341-2343.
20. Wolfe, K.; Wu, X.; Liu, R. H. Antioxidant activity of apple peels. J. Agric. Food Chem. 2003, 51, 609-614.
21. Sweeny, J. G.; Iacobucci, G. A. Synthesis of anthocyanins. II. The synthesis of 3-deoxyanthocyanidins from 5-hydroxy-flavanones. Tetrahedron 1977, 33, 2927-2932.
22. Sweeny, J. G.; Iacobucci, G. A. Synthesis of anthocyanins. I. The oxidative generation of flavylium cations using benzoquinines. Tetrahedron 1977, 33, 2923-2926.
23. Sweeny, J. G.; Iacobucci, G. A. Synthesis of anthocyanins. III. Total synthesis of apigeninidin and luteolinidin chlorides. Tetrahedron 1981, 37, 1481-1483.
24. Jurd, L. Quinone oxidation of flavenes and Ilavan-3,4-diols. Chem. Ind. 1966, 40, 1683-1684.
25. Sarkar, S. K.; Howarth, R. E. Specificity of vanillin test for flavonols. J. Agric. Food Chem. 1976, 24, 317-320.
26. Broadhurst, R. B.; Jones, W. T. Analysis of condensed tannins using acidified vanillin. J. Sci. Food Agric. 1978, 29, 788-794.
27. Sun, J.; Chu, Y. F.; Wu, X. Z.; Liu, R. H. Antioxidant and antiproliferative activities of common fruits. J. Agric. Food Chem. 2002, 50, 7449-7454.