Quantitative real-time PCR monitoring of Escherichia coli and Clostridium perfringens with oral administration of Lactobacillus plantarum strain Lq80 to weaning piglets

Animal Science Journal

Volumn 79, Issue 6, 2008, Pages 737-744

  Takahashi, Shunsuke ^a   Yoshida, Yoko ^a   Nakanishi, Nobuo ^b   Tsukahara, Takamitsu ^a,c   Ushida, Kazunari ^a  

^a KYOTO PREFECTURAL UNIVERSITY   (Japan)

^b Kyodoken Institute   (Japan)

^c Ujitawara cho   (Japan)

Author keywords

Enteropathogen; L. plantarum strain Lq80; Oral administration; Probiotic; Quantitative real time PCR

Indexed keywords

CLOSTRIDIUM PERFRINGENS; ESCHERICHIA COLI; LACTOBACILLUS; LACTOBACILLUS PLANTARUM;

EID: 55349114052     PISSN: 13443941     EISSN: 17400929     Source Type: Journal    
DOI: 10.1111/j.1740-0929.2008.00588.x     Document Type: Article

References (42)

1. Abe F, Ishibashi N, Shimamura S. 1995. Effect of administration of bifidobacteria and lactic acid bacteria to newborn calves and piglets. Journal of Dairy Science 78, 2838 2846.
2. Barton MD. 2000. Antibiotic use in animal feed and its impact on human health. Nutrition Research Reviews 13, 279 299.
3. Bujalance C, Jimenez-Valera M, Moreno E, Ruiz-Bravo A. 2006. A selective differential medium for Lactobacillus plantarum. Journal of Microbiological Methods 66, 572 575.
4. Castillo M, Martin-Orue SM, Manzanilla EG, Badiola I, Martin M, Gasa J. 2006. Quantification of total bacteria, enterobacteria and lactobacilli populations in pig digesta by real-time PCR. Veterinary Microbiology 114, 165 170.
5. Drisko JA, Giles CK, Bischoff BJ. 2003. Probiotics in health maintenance and disease prevention. Alternative Medicine Review 8, 143 155.
6. Fach P, Popoff MR. 1997. Detection of enterotoxigenic Clostridium perfringens in food and fecal samples with a duplex PCR and the slide latex agglutination test. Applied and Environmental Microbiology 63, 4232 4236.
7. Franck SM, Bosworth BT, Moon HW. 1998. Multiplex PCR for enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains from calves. Journal of Clinical Microbiology 36, 1795 1797.
8. Fukushima H, Tsunomori Y, Seki R. 2003. Duplex real-time SYBR green PCR assays for detection of 17 species of food- or waterborne pathogens in stools. Journal of Clinical Microbiology 41, 5134 5146.
9. Gionchetti P, Rizzello F, Lammers KM, Morselli C, Sollazzi L, Davies S, Tambasco R, Calabrese C, Campieri M. 2006. Antibiotics and probiotics in treatment of inflammatory bowel disease. World Journal of Gastroenterology. 12, 3306 3313.
10. Godon JJ, Zumstein E, Dabert P, Habouzit F, Moletta R. 1997. Molecular microbial diversity of an anaerobic digestor as determined by small-subunit rDNA sequence analysis. Applied and Environmental Microbiology 63, 2802 2813.
11. Hampson DJ. 1994. Postweaning Escherichia coli diarrhoea in pigs. In : Gyles CL (ed.), Escherichia Coli in Domestic Animals and Humans, 2nd edn, pp. 171 191. CAB International, Wallingford.
12. Hatheway CL. 1990. Toxigenic clostridia. Clinical Microbiology Reviews 3, 66 98.
13. Holzapfel WH, Haberer P, Geisen R, Björkroth J, Schillinger U. 2001. Taxonomy and important features of probiotics food and nutrition. American Journal of Clinical Nutrition 73, 365 373.
14. Huijsdens XW, Linskens RK, Mak M, Meuwissen SG, Vandenbroucke-Grauls CM, Savelkoul PH. 2002. Quantification of bacteria adherent to gastrointestinal mucosa by real-time PCR. Journal of Clinical Microbiology 40, 4423 4427.
15. Ishida-Fujii K, Sato R, Goto S, Yang XP, Kuboki H, Hirano S, Sato M. 2007. Prevention of pathogenic Escherichia coli infection in mice and stimulation of macrophage activation in rats by an oral administration of probiotic Lactobacillus casei I-5. Bioscience, Biotechnology, and Biochemistry 71, 866 873.
16. Kim PI, Jung MY, Chang YH, Kim S, Kim SJ, Park YH. 2007. Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract. Applied Microbiology and Biotechnology 74, 1103 1111.
17. Maldonado A, Ruiz-Barba JL, Jiménez-Díaz R. 2003. Purification and genetic characterization of plantaricin NC8, a novel coculture-inducible two-peptide bacteriocin from Lactobacillus plantarum NC8. Applied Microbiology and Biotechnology 69, 383 389.
18. Matijasic BB, Stojkovic S, Rogelj I. 2006. Survival and in vivo adhesion of human isolates Lactobacillus gasseri LF221 and K7 in weaned piglets and their effects on coliforms, clostridia and lactobacilli viable counts in faeces and mucosa. Journal of Dairy Research 73, 417 422.
19. Matsuki T, Watanabe K, Fujimoto J, Takada T, Tanaka R. 2004. Use of 16S rRNA gene-targeted group-specific primers for real-time PCR analysis of predominant bacteria in human feces. Applied and Environmental Microbiology 70, 7220 7228.
20. Melin L, Mattsson S, Katouli M, Wallgren P. 2004. Development of post-weaning diarrhoea in piglets. Relation to presence of Escherichia coli strains and rotavirus. Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health. 51, 12 22.
21. Moeser AJ, Blikslager AT. 2007. Mechanisms of porcine diarrheal disease. Journal of the American Veterinary Medical Association 231, 56 67.
22. Nataro JP, Kaper JB. 1998. Diarrheagenic Escherichia coli. Clinical Microbiology Reviews 11, 142 201.
23. Nomoto K. 2005. Prevention of infections by probiotics. Journal of Bioscience and Bioengineering 100, 583 592.
24. Ohashi Y, Inoue R, Tanaka K, Matsuki T, Umesaki Y, Ushida K. 2001. Lactobacillus casei strain Shirota-fermented milk stimulates indigenous Lactobacillus in the pig intestine. Journal of Nutritional Science and Vitaminology 47, 172 176.
25. Ohashi Y, Tokunaga M, Taketomo N, Ushida K. 2007. Stimulation of indigenous lactobacilli by the fermented milk prepared with probiotic bacterium, Lactobacillus delbrueckii subsp. bulgaricus strain 2038, in the pigs. Journal of Nutritional Science and Vitaminology 53, 82 86.
26. Ohashi Y, Umesaki Y, Ushida K. 2004. Transition of the probiotic bacteria, Lactobacillus casei strain Shirota, in the gastrointestinal tract of a pig. International Journal of Food Microbiology 96, 61 66.
27. Ouwehand AC, Salminen S, Isolauri E. 2002. Probiotics: an overview of beneficial effects. Antonie Van Leeuwenhoek 82, 279 289.
28. Parvez S, Malik KA, Ah Kang S, Kim HY. 2006. Probiotics and their fermented food products are beneficial for health. Journal of Applied Microbiology 100, 1171 1185.
29. Patterson JA, Burkholder KM. 2003. Application of prebiotics and probiotics in poultry production. Poultry Science 82, 627 631.
30. Penders J, Vink C, Driessen C, London N, Thijs C, Stobberingh EE. 2005. Quantification of Bifidobacterium spp., Escherichia coli and Clostridium difficile in faecal samples of breast-fed and formula-fed infants by real-time PCR. FEMS Microbiology Letters 243, 141 147.
31. Perdigon G, Maldonado Galdeano C, Valdez JC, Medici M. 2002. Interaction of lactic acid bacteria with the gut immune system. European Journal of Clinical Nutrition 56, 21 26.
32. Reid G, Hammond JA. 2005. Probiotics. Some evidence of their effectiveness. Canadian Family Physician 51, 1487 1493.
33. Reid G, Howard J, Gan BS. 2001. Can bacterial interference prevent infection? Trends in Microbiology 9, 424 428.
34. Roberfroid MB. 2000. Prebiotics and probiotics: are they functional foods? American Journal of Clinical Nutrition 71, 1682 1687.
35. Songer JG, Uzal FA. 2005. Clostridial enteric infections in pigs. Journal of Veterinary Diagnostic Investigation 17, 528 536.
36. Takahashi S, Egawa Y, Simojo N, Tsukahara T, Ushida K. 2007. Oral administration of Lactobacillus plantarum strain Lq80 to weaning piglets stimulates the growth of indigenous lactobacilli to modify the lactobacillal population. Journal of General and Applied Microbiology 53, 325 332.
37. Topping DL, Clifton PM. 2001. Short-chain fatty acids and human colonic function: roles of resistant starch and nonstarch polysaccharides. Physiological Reviews 81, 1031 1064.
38. Ushida K, Inoue R, Shimojo N, Takahashi S, Kameue C, Tsukahara T. 2006. Oral dose of Lactobacillus plantarum and Megasphaera elsdenii increased intestinal IgA secretion, colonic butyrate and growth of colonic mucosa in piglets. Reproduction Nutrition Development 46 (Suppl 1 S76.
39. Vu-Khac H, Holoda E, Pilipcinec E, Blanco M, Blanco JE, Dahbi G, Mora A, Lopez C, Gonzalez EA, Blanco J. 2007. Serotypes, virulence genes, and PFGE profiles of Escherichia coli isolated from piglets with diarrhoea in Slovakia. Veterinary Journal 174, 176 187.
40. Wise MG, Siragusa GR. 2005. Quantitative detection of Clostridium perfringens in the broiler fowl gastrointestinal tract by real-time PCR. Applied and Environmental Microbiology 71, 3911 3916.
41. Yanai H. 1998. Excel-Toukei, 1st edn. Seiunsha, Tokyo. (In Japanese)
42. Zoetendal EG, Collier CT, Koike S, Mackie RI, Gaskins HR. 2004. Molecular ecological analysis of the gastrointestinal microbiota. Journal of Nutrition 134, 465 472.