Quantification of Bifidobacterium spp., Escherichia coli and Clostridium difficile in faecal samples of breast-fed and formula-fed infants by real-time PCR

FEMS Microbiology Letters

Volumn 243, Issue 1, 2005, Pages 141-147

  Penders, John ^a   Vink, Cornelis ^b   Driessen, Christel ^b   London, Nancy ^b   Thijs, Carel ^a   Stobberingh, Ellen E ^b  

^a MAASTRICHT UNIVERSITY   (Netherlands)

^b MAASTRICHT UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Bifidobacterium; Breastfeeding; Clostridium difficile; Escherichia coli; Infant formula; Intestinal microbiota; Real time PCR

Indexed keywords

ARTICLE; ARTIFICIAL MILK; BACTERIAL COUNT; BACTERIUM COLONY; BACTERIUM IDENTIFICATION; BIFIDOBACTERIUM; BREAST FEEDING; CLOSTRIDIUM DIFFICILE; COLONY FORMING UNIT; CONTROLLED STUDY; ESCHERICHIA COLI; FECES ANALYSIS; FEMALE; HUMAN; INFANT; INFANT FEEDING; INTESTINE FLORA; MALE; NONHUMAN; PREVALENCE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION;

BIFIDOBACTERIUM; BOTTLE FEEDING; BREAST FEEDING; CLOSTRIDIUM DIFFICILE; COLONY COUNT, MICROBIAL; ESCHERICHIA COLI; FECES; FEMALE; HUMANS; INFANT; INFANT FOOD; INFANT FORMULA; MALE; MILK, HUMAN; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

BIFIDOBACTERIUM; CLOSTRIDIUM; CLOSTRIDIUM DIFFICILE; ESCHERICHIA COLI; MICROBIOTA;

EID: 12844251441     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.femsle.2004.11.052     Document Type: Article

References (36)

1. F. Guarner, and J.R. Malagelada Gut flora in health and disease Lancet 361 2003 512 519
2. N. Sudo, S. Sawamura, K. Tanaka, Y. Aiba, C. Kubo, and Y. Koga The requirement of intestinal bacterial flora for the development of an IgE production system fully susceptible to oral tolerance induction J. Immunol. 1950 159 1997 1739 1745
3. M. Kalliomaki, S. Salminen, H. Arvilommi, P. Kero, P. Koskinen, and E. Isolauri Probiotics in primary prevention of atopic disease: a randomised placebo-controlled trial Lancet 357 2001 1076 1079
4. P.V. Kirjavainen, E. Apostolou, T. Arvola, S.J. Salminen, G.R. Gibson, and E. Isolauri Characterizing the composition of intestinal microflora as a prospective treatment target in infant allergic disease FEMS Immunol. Med. Microbiol. 32 2001 1 7
5. R.B. Sartor Therapeutic manipulation of the enteric microflora in inflammatory bowel diseases: antibiotics, probiotics, and prebiotics Gastroenterology 126 2004 1620 1633
6. P.M. Heavey, and I.R. Rowland The gut microbiology of the developing infant: microbiology and metabolism Microb. Ecol. Health D 11 1999 75 83
7. X.W. Huijsdens, R.K. Linskens, M. Mak, S.G. Meuwissen, C.M. Vandenbroucke Grauls, and P.H. Savelkoul Quantification of bacteria adherent to gastrointestinal mucosa by real-time PCR J. Clin. Microbiol. 40 2002 4423 4427
8. J.H. Apajalahti, A. Kettunen, P.H. Nurminen, H. Jatila, and W.E. Holben Selective plating underestimates abundance and shows differential recovery of bifidobacterial species from human feces Appl. Environ. Microbiol. 69 2003 5731 5735
9. K.H. Wilson, and R.B. Blitchington Human colonic biota studied by ribosomal DNA sequence analysis Appl. Environ. Microbiol. 62 1996 2273 2278
10. P. Seksik, L. Rigottier-Gois, G. Gramet, M. Sutren, P. Pochart, P. Marteau, R. Jian, and J. Dore Alterations of the dominant faecal bacterial groups in patients with Crohn's disease of the colon Gut 52 2003 237 242
11. E.G. Zoetendal, A.D. Akkermans, and W.M. De Vos Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria Appl. Environ. Microbiol. 64 1998 3854 3859
12. R.M. Satokari, E.E. Vaughan, A.D. Akkermans, M. Saarela, and W.M. de Vos Bifidobacterial diversity in human feces detected by genus-specific PCR and denaturing gradient gel electrophoresis Appl. Environ. Microbiol. 67 2001 504 513
13. G.J. Jansen, A.C. Wildeboer-Veloo, R.H. Tonk, A.H. Franks, and G.W. Welling Development and validation of an automated, microscopy-based method for enumeration of groups of intestinal bacteria J. Microbiol. Meth. 37 1999 215 221
14. P.S. Langendijk, F. Schut, G.J. Jansen, G.C. Raangs, G.R. Kamphuis, M.H. Wilkinson, and G.W. Welling Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples Appl. Environ. Microbiol. 61 1995 3069 3075
15. C.A. Heid, J. Stevens, K.J. Livak, and P.M. Williams Real time quantitative PCR Genome Res. 6 1996 986 994
16. T. Requena, J. Burton, T. Matsuki, K. Munro, M.A. Simon, R. Tanaka, K. Watanabe, and G.W. Tannock Identification, detection, and enumeration of human bifidobacterium species by PCR targeting the transaldolase gene Appl. Environ. Microbiol. 68 2002 2420 2427
17. T. Matsuki, K. Watanabe, J. Fujimoto, Y. Kado, T. Takada, K. Matsumoto, and R. Tanaka Quantitative PCR with 16S rRNA-gene-targeted species-specific primers for analysis of human intestinal bifidobacteria Appl. Environ. Microbiol. 70 2004 167 173
18. S.J. Ott, M. Musfeldt, U. Ullmann, J. Hampe, and S. Schreiber Quantification of intestinal bacterial populations by real-time PCR with a universal primer set and minor groove binder probes: a global approach to the enteric flora J. Clin. Microbiol. 42 2004 2566 2572
19. M. Gueimonde, S. Tolkko, T. Korpimaki, and S. Salminen New real-time quantitative PCR procedure for quantification of bifidobacteria in human fecal samples Appl. Environ. Microbiol. 70 2004 4165 4169
20. S.J. Kaptein, P.S. Beisser, Y.K. Gruijthuijsen, K.G. Savelkouls, K.W. van Cleef, E. Beuken, G.E. Grauls, C.A. Bruggeman, and C. Vink The rat cytomegalovirus R78 G protein-coupled receptor gene is required for production of infectious virus in the spleen J. Gen. Virol. 84 2003 2517 2530
21. S. Yang, S. Lin, G.D. Kelen, T.C. Quinn, J.D. Dick, C.A. Gaydos, and R.E. Rothman Quantitative multiprobe PCR assay for simultaneous detection and identification to species level of bacterial pathogens J. Clin. Microbiol. 40 2002 3449 3454
22. C.L. Bullen, P.V. Tearle, and A. Willis Bifidobacteria in the intestinal tract of infants J. Med. Microbiol. 9 1976 325 333
23. C.L. Bullen, P.V. Tearle, and M.G. Stewart The effect of "humanised" milks and supplemented breast feeding on the faecal flora of infants J. Med. Microbiol. 10 1977 403 413
24. P.L. Stark, and A. Lee The microbial ecology of the large bowel of breast-fed and formula-fed infants during the first year of life J. Med. Microbiol. 15 1982 189 203
25. B. Kleessen, H. Bunke, K. Tovar, J. Noack, and G. Sawatzki Influence of two infant formulas and human milk on the development of the faecal flora in newborn infants Acta Paediatr. 84 1995 1347 1356
26. Y. Benno, K. Sawada, and T. Mitsuoka The intestinal microflora of infants: composition of fecal flora in breast-fed and bottle-fed infants Microbiol. Immunol. 28 1984 975 986
27. M.A. Hall, C.B. Cole, S.L. Smith, R. Fuller, and C.J. Rolles Factors influencing the presence of faecal lactobacilli in early infancy Arch. Dis. Child. 65 1990 185 188
28. B. Lundequist, C.E. Nord, and J. Winberg The composition of the faecal microflora in breastfed and bottle fed infants from birth to eight weeks Acta. Paediatr. Scand. 74 1985 45 51
29. H. Yoshioka, K. Iseki, and K. Fujita Development and differences of intestinal flora in the neonatal period in breast-fed and bottle-fed infants Pediatrics 72 1983 317 321
30. J.U. Oguike, and A.C. Emeruwa Incidence of Clostridium difficile in infants in rural and urban areas of Nigeria Microbiologica 13 1990 267 271
31. S. Wongwanich, P. Pongpech, C. Dhiraputra, S. Huttayananont, and P. Sawanpanyalert Characteristics of Clostridium difficile strains isolated from asymptomatic individuals and from diarrheal patients Clin. Microbiol. Infect. 7 2001 438 441
32. K. Tullus, B. Aronsson, S. Marcus, and R. Mollby Intestinal colonization with Clostridium difficile in infants up to 18 months of age Eur. J. Clin. Microbiol. 8 1989 390 393
33. V. Merida, J. Moerman, J. Colaert, P. Lemmens, and J. Vandepitte Significance of Clostridium difficile and its cytotoxin in children Eur. J. Pediatr. 144 1986 494 496
34. H.E. Larson, F.E. Barclay, P. Honour, and I.D. Hill Epidemiology of Clostridium difficile in infants J. Infect. Dis. 146 1982 727 733
35. P. Naaber, K. Klaus, E. Sepp, B. Bjorksten, and M. Mikelsaar Colonization of infants and hospitalized patients with Clostridium difficile and lactobacilli Clin. Infect. Dis. 25 Suppl. 2 1997 S189 S190
36. J.M. Neefs, Y. Van de Peer, P. De Rijk, A. Goris, and R. De Wachter Compilation of small ribosomal subunit RNA sequences Nucleic Acids Res. 19 Suppl. 1991 1987 2015