메뉴 건너뛰기




Volumn 1240, Issue , 2008, Pages 62-72

An automated segmentation methodology for quantifying immunoreactive puncta number and fluorescence intensity in tissue sections

Author keywords

Fluorescent immunohistochemistry; Immunoreactive puncta; Quantitative fluorescence microscopy; Segmentation; Synaptic structure

Indexed keywords

ANIMAL TISSUE; ARTICLE; AUTOMATION; CONFOCAL MICROSCOPY; FEMALE; IMMUNOFLUORESCENCE; IMMUNOREACTIVITY; MALE; METHODOLOGY; MONKEY; MORPHOLOGY; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SYNAPSE; TISSUE SECTION;

EID: 54949108524     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.brainres.2008.08.060     Document Type: Article
Times cited : (38)

References (25)
  • 1
    • 3042647610 scopus 로고    scopus 로고
    • The mGluR theory of fragile X mental retardation
    • Bear M.F., Huber K.M., and Warren S.T. The mGluR theory of fragile X mental retardation. Trends Neurosci. 27 (2004) 370-377
    • (2004) Trends Neurosci. , vol.27 , pp. 370-377
    • Bear, M.F.1    Huber, K.M.2    Warren, S.T.3
  • 2
    • 0024436632 scopus 로고
    • Characterization of internalization and endosome formation of epidermal growth factor in transfected NIH-3T3 cells by computerized image-intensified three-dimensional fluorescence microscopy
    • Benveniste M., Schlessinger J., and Kam Z. Characterization of internalization and endosome formation of epidermal growth factor in transfected NIH-3T3 cells by computerized image-intensified three-dimensional fluorescence microscopy. J. Cell Biol. 109 (1989) 2105-2115
    • (1989) J. Cell Biol. , vol.109 , pp. 2105-2115
    • Benveniste, M.1    Schlessinger, J.2    Kam, Z.3
  • 3
    • 33644759332 scopus 로고    scopus 로고
    • Automated criteria-based selection and analysis of fluorescent synaptic puncta
    • Bergsman J.B., Krueger S.R., and Fitzsimonds R.M. Automated criteria-based selection and analysis of fluorescent synaptic puncta. J. Neurosci. Methods 152 (2006) 32-39
    • (2006) J. Neurosci. Methods , vol.152 , pp. 32-39
    • Bergsman, J.B.1    Krueger, S.R.2    Fitzsimonds, R.M.3
  • 4
    • 0030599373 scopus 로고    scopus 로고
    • The hierarchical development of monkey visual cortical regions as revealed by the maturation of parvalbumin-immunoreactive neurons
    • Cond Ç.F., Lund J.S., and Lewis D.A. The hierarchical development of monkey visual cortical regions as revealed by the maturation of parvalbumin-immunoreactive neurons. Dev. Brain Res. 96 (1996) 261-276
    • (1996) Dev. Brain Res. , vol.96 , pp. 261-276
    • Cond, Ç.F.1    Lund, J.S.2    Lewis, D.A.3
  • 5
    • 0141656259 scopus 로고    scopus 로고
    • Postnatal development of pre- and post-synaptic GABA markers at chandelier cell inputs to pyramidal neurons in monkey prefrontal cortex
    • Cruz D.A., Eggan S.M., and Lewis D.A. Postnatal development of pre- and post-synaptic GABA markers at chandelier cell inputs to pyramidal neurons in monkey prefrontal cortex. J. Comp. Neurol. 465 (2003) 385-400
    • (2003) J. Comp. Neurol. , vol.465 , pp. 385-400
    • Cruz, D.A.1    Eggan, S.M.2    Lewis, D.A.3
  • 6
    • 0036114317 scopus 로고    scopus 로고
    • Postnatal development of parvalbumin- and GABA transporter-immunoreactive axon terminals in monkey prefrontal cortex
    • Erickson S.L., and Lewis D.A. Postnatal development of parvalbumin- and GABA transporter-immunoreactive axon terminals in monkey prefrontal cortex. J. Comp. Neurol. 448 (2002) 186-202
    • (2002) J. Comp. Neurol. , vol.448 , pp. 186-202
    • Erickson, S.L.1    Lewis, D.A.2
  • 7
    • 0028324791 scopus 로고
    • Comparative localization of two forms of glutamic acid decarboxylase and their mRNAs in rat brain supports the concept of functional differences between the forms
    • Esclapez M., Tillakaratne N.J., Kaufman D.L., Tobin A.J., and Houser C.R. Comparative localization of two forms of glutamic acid decarboxylase and their mRNAs in rat brain supports the concept of functional differences between the forms. J. Neurosci. 14 (1994) 1834-1855
    • (1994) J. Neurosci. , vol.14 , pp. 1834-1855
    • Esclapez, M.1    Tillakaratne, N.J.2    Kaufman, D.L.3    Tobin, A.J.4    Houser, C.R.5
  • 8
    • 0027336431 scopus 로고
    • Different distributions of GAD65 and GAD67 mRNAs suggest that the two glutamate decarboxylases play distinctive functional roles
    • Feldblum S., Erlander M.G., and Tobin A.J. Different distributions of GAD65 and GAD67 mRNAs suggest that the two glutamate decarboxylases play distinctive functional roles. J. Neurosci. Res 34 (1993) 689-706
    • (1993) J. Neurosci. Res , vol.34 , pp. 689-706
    • Feldblum, S.1    Erlander, M.G.2    Tobin, A.J.3
  • 9
    • 0029417183 scopus 로고
    • Comparative distribution of GAD65 and GAD67 mRNAs and proteins in the rat spinal cord supports a differential regulation of these two glutamate decarboxylases in vivo
    • Feldblum S., Dumoulin A., Anoal M., Sandillon F., and Privat A. Comparative distribution of GAD65 and GAD67 mRNAs and proteins in the rat spinal cord supports a differential regulation of these two glutamate decarboxylases in vivo. J. Neurosci. Res. 42 (1995) 742-757
    • (1995) J. Neurosci. Res. , vol.42 , pp. 742-757
    • Feldblum, S.1    Dumoulin, A.2    Anoal, M.3    Sandillon, F.4    Privat, A.5
  • 10
    • 34347217102 scopus 로고    scopus 로고
    • Immunocytochemistry and quantification of protein colocalization in cultured neurons
    • Glynn M.W., and McAllister A.K. Immunocytochemistry and quantification of protein colocalization in cultured neurons. Nat. Protoc. 1 (2006) 1287-1296
    • (2006) Nat. Protoc. , vol.1 , pp. 1287-1296
    • Glynn, M.W.1    McAllister, A.K.2
  • 11
    • 0025689251 scopus 로고
    • Temporal and spatial coordination of chromosome movement, spindle formation, and nuclear envelope breakdown during prometaphase in Drosophila melanogaster embryos
    • Hiraoka Y., Agard D.A., and Sedat J.W. Temporal and spatial coordination of chromosome movement, spindle formation, and nuclear envelope breakdown during prometaphase in Drosophila melanogaster embryos. J. Cell Biol. 111 (1990) 2815-2828
    • (1990) J. Cell Biol. , vol.111 , pp. 2815-2828
    • Hiraoka, Y.1    Agard, D.A.2    Sedat, J.W.3
  • 13
    • 0033599967 scopus 로고    scopus 로고
    • Parvalbumin-immunoreactive axon terminals in macaque monkey and human prefrontal cortex: laminar, regional and target specificity of Type I and Type II synapses
    • Melchitzky D.S., Sesack S.R., and Lewis D.A. Parvalbumin-immunoreactive axon terminals in macaque monkey and human prefrontal cortex: laminar, regional and target specificity of Type I and Type II synapses. J. Comp. Neurol. 408 (1999) 11-22
    • (1999) J. Comp. Neurol. , vol.408 , pp. 11-22
    • Melchitzky, D.S.1    Sesack, S.R.2    Lewis, D.A.3
  • 14
    • 0035426751 scopus 로고    scopus 로고
    • Analysis of complex brain disorders with gene expression microarrays: schizophrenia as a disease of the synapse
    • Mirnics K., Middleton F.A., Lewis D.A., and Levitt P. Analysis of complex brain disorders with gene expression microarrays: schizophrenia as a disease of the synapse. Trends Neurosci. 24 (2001) 479-486
    • (2001) Trends Neurosci. , vol.24 , pp. 479-486
    • Mirnics, K.1    Middleton, F.A.2    Lewis, D.A.3    Levitt, P.4
  • 15
    • 36549030392 scopus 로고    scopus 로고
    • Evaluating performance in three-dimensional fluorescence microscopy
    • Murray J.M., Appleton P.L., Swedlow J.R., and Waters J.C. Evaluating performance in three-dimensional fluorescence microscopy. J. Microsc. 228 (2007) 390-405
    • (2007) J. Microsc. , vol.228 , pp. 390-405
    • Murray, J.M.1    Appleton, P.L.2    Swedlow, J.R.3    Waters, J.C.4
  • 16
    • 0027361010 scopus 로고
    • Postnatal development of the cholecystokinin innervation of monkey prefrontal cortex
    • Oeth K.M., and Lewis D.A. Postnatal development of the cholecystokinin innervation of monkey prefrontal cortex. J. Comp. Neurol. 336 (1993) 400-418
    • (1993) J. Comp. Neurol. , vol.336 , pp. 400-418
    • Oeth, K.M.1    Lewis, D.A.2
  • 17
    • 0028382485 scopus 로고
    • Background rejection and signal-to-noise optimization in confocal and alternative fluorescence microscopes
    • Sandison D.R., and Webb W.W. Background rejection and signal-to-noise optimization in confocal and alternative fluorescence microscopes. Applied Optics 33 (1994) 603-615
    • (1994) Applied Optics , vol.33 , pp. 603-615
    • Sandison, D.R.1    Webb, W.W.2
  • 18
    • 0037174618 scopus 로고    scopus 로고
    • Alzheimer's disease is a synaptic failure
    • Selkoe D.J. Alzheimer's disease is a synaptic failure. Science 298 (2002) 789-791
    • (2002) Science , vol.298 , pp. 789-791
    • Selkoe, D.J.1
  • 19
    • 84900170520 scopus 로고    scopus 로고
    • Comparison of widefield/deconvolution and confocal microscopy for three-dimensional imaging
    • Pawley J.B. (Ed), Springer, New York
    • Shaw P.J. Comparison of widefield/deconvolution and confocal microscopy for three-dimensional imaging. In: Pawley J.B. (Ed). Handbook of Biological Confocal Microscopy (2006), Springer, New York 453-467
    • (2006) Handbook of Biological Confocal Microscopy , pp. 453-467
    • Shaw, P.J.1
  • 20
    • 0021234096 scopus 로고
    • The unbiased estimation of number and sizes of arbitrary particles using the disector
    • Sterio D.C. The unbiased estimation of number and sizes of arbitrary particles using the disector. J. Microsc. 134 (1984) 127-136
    • (1984) J. Microsc. , vol.134 , pp. 127-136
    • Sterio, D.C.1
  • 21
    • 0032740915 scopus 로고    scopus 로고
    • Localization of cells preferentially expressing GAD(67) with negligible GAD(65) transcripts in the rat hippocampus. A double in situ hybridization study
    • Stone D.J., Walsh J., and Benes F.M. Localization of cells preferentially expressing GAD(67) with negligible GAD(65) transcripts in the rat hippocampus. A double in situ hybridization study. Brain Res. Mol. Brain Res. 71 (1999) 201-209
    • (1999) Brain Res. Mol. Brain Res. , vol.71 , pp. 201-209
    • Stone, D.J.1    Walsh, J.2    Benes, F.M.3
  • 22
    • 24044487619 scopus 로고    scopus 로고
    • Determination of absolute protein numbers in single synapses by a GFP-based calibration technique
    • Sugiyama Y., Kawabata I., Sobue K., and Okabe S. Determination of absolute protein numbers in single synapses by a GFP-based calibration technique. Nat. Methods 2 (2005) 677-684
    • (2005) Nat. Methods , vol.2 , pp. 677-684
    • Sugiyama, Y.1    Kawabata, I.2    Sobue, K.3    Okabe, S.4
  • 23
    • 0037133239 scopus 로고    scopus 로고
    • Measuring tubulin content in Toxoplasma gondii: a comparison of laser-scanning confocal and wide-field fluorescence microscopy
    • Swedlow J.R., Hu K., Andrews P.D., Roos D.S., and Murray J.M. Measuring tubulin content in Toxoplasma gondii: a comparison of laser-scanning confocal and wide-field fluorescence microscopy. Proc. Natl. Acad. Sci. U. S. A. 99 (2002) 2014-2019
    • (2002) Proc. Natl. Acad. Sci. U. S. A. , vol.99 , pp. 2014-2019
    • Swedlow, J.R.1    Hu, K.2    Andrews, P.D.3    Roos, D.S.4    Murray, J.M.5
  • 24
    • 18444387409 scopus 로고    scopus 로고
    • Performance comparison between the high-speed Yokogawa spinning disc confocal system and single-point scanning confocal systems
    • Wang E., Babbey C.M., and Dunn K.W. Performance comparison between the high-speed Yokogawa spinning disc confocal system and single-point scanning confocal systems. J. Microsc. 218 (2005) 148-159
    • (2005) J. Microsc. , vol.218 , pp. 148-159
    • Wang, E.1    Babbey, C.M.2    Dunn, K.W.3
  • 25
    • 24344477277 scopus 로고    scopus 로고
    • Localization of calcium-binding proteins in physiologically and morphologically characterized interneurons of monkey dorsolateral prefrontal cortex
    • Zaitsev A.V., Gonzalez-Burgos G., Povysheva N.V., Kroner S., Lewis D.A., and Krimer L.S. Localization of calcium-binding proteins in physiologically and morphologically characterized interneurons of monkey dorsolateral prefrontal cortex. Cereb. Cortex 15 (2005) 1178-1186
    • (2005) Cereb. Cortex , vol.15 , pp. 1178-1186
    • Zaitsev, A.V.1    Gonzalez-Burgos, G.2    Povysheva, N.V.3    Kroner, S.4    Lewis, D.A.5    Krimer, L.S.6


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.