The desensitization gating of the MthK K+ channel is governed by its cytoplasmic amino terminus

PLoS Biology

Volumn 6, Issue 10, 2008, Pages 2286-2296

  Kuo, Mario Meng Chiang ^a   Maslennikov, Innokentiy ^a   Molden, Brent ^a   Choe, Senyon ^a  

^a SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

POTASSIUM CHANNEL; POTASSIUM CHANNEL MTHK; SYNTHETIC PEPTIDE; UNCLASSIFIED DRUG; ARCHAEAL PROTEIN; PEPTIDE;

AMINO TERMINAL SEQUENCE; ARTICLE; CHANNEL GATING; CONTROLLED STUDY; CYTOPLASM; GENE DELETION; HYDROPHOBICITY; MUTAGENESIS; NONHUMAN; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTON NUCLEAR MAGNETIC RESONANCE; AMINO ACID SEQUENCE; CHEMICAL PHENOMENA; CHEMISTRY; ELECTROPHYSIOLOGY; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PH; PHYSIOLOGY; SPHEROPLAST;

AMINO ACID SEQUENCE; ARCHAEAL PROTEINS; CHROMATOGRAPHY, GEL; CYTOPLASM; ELECTROPHYSIOLOGY; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR SEQUENCE DATA; MUTATION; PEPTIDES; POTASSIUM CHANNELS; SPHEROPLASTS;

EID: 54949088618     PISSN: 15449173     EISSN: 15457885     Source Type: Journal    
DOI: 10.1371/journal.pbio.0060223     Document Type: Article

References (36)

1. Gu C, Jan YN, Jan LY (2003) A conserved domain in axonal targeting of Kv1 (Shaker) voltage-gated potassium channels. Science 301: 646-649.
2. Hille B (2001) Ion channels of excitable membranes. Sunderland (Massachusetts): Sinauer Associates. 814 p.
3. Nishida M, Cadene M, Chait BT, MacKinnon R (2007) Crystal structure of a Kir3.1-prokaryotic Kir channel chimera. EMBO J 26: 4005-4015.
4. Pegan S, Arrabit C, Zhou W, Kwiatkowski W, Collins A, et al. (2005) Cytoplasmic domain structures of Kir2.1 and Kir3.1 show sites for modulating gating and rectification. Nat Neurosci 8: 279-287.
5. +-channel flux: a structural perspective. Trends Biochem Sci 29: 39-45.
6. + channel and demonstration of its presence in the human BK channel. Neuron 29: 593-601.
7. Roosild TP, Miller S, Booth IR, Choe S (2002) A mechanism of regulating transmembrane potassium flux through a ligand-mediated conformational switch. Cell 109: 781-791.
8. + channels: From crystal structures to diversity. FEMS Microbiol Rev 29: 961-985.
9. Jiang YX, Lee A, Chen JY, Cadene M, Chait BT, et al. (2002) Crystal structure and mechanism of a calcium-gated potassium channel. Nature 417: 515-522.
10. + channels. Cell 126: 1161-1173.
11. Kuo MM-C, Baker KA, Wong L, Choe S (2007) Dynamic oligomeric conversions of the cytoplasmic RCK domains mediate MthK potassium channel activity. Proc Natl Acad Sci U S A 104: 2151-2156.
12. + channel with and without the gating ring. J Gen Physiol 129: 109-120.
13. 2+ on gating ring stability. J Biol Chem 280: 41716-41724.
14. Parfenova LV, Crane BM, Rothberg BS (2006) Modulation of MthK potassium channel activity at the intracellular entrance to the pore. J Biol Chem 281: 21131-21138.
15. Zadek B, Nimigean CM (2006) Calcium-dependent gating of MthK, a prokaryotic potassium channel. J Gen Physiol 127: 673-685.
16. Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, et al. (2005) NMR structure of Mistic, a membrane-integrating protein for membrane protein expression. Science 307: 1317-1321.
17. Aldrich RW (2001) Fifty years of inactivation. Nature 411: 643-644.
18. Wuthrich K (1986) NMR of proteins and nucleic acids. New York: John Wiley & Sons. 320 p.
19. Chakrapani S, Perozo E (2007) How to gate an ion channel: lessons from MthK. Nat Struct Mol Biol 14: 180-182.
20. Delcour AH, Martinac B, Adler J, Kung C (1989) Modified reconstitution method used in patch-clamp studies of Escherichia coli ion channels. Biophys J 56: 631-636.
21. Blount P, Sukharev SI, Moe PC, Martinac B, Kung C (1999) Mechanosensitive channels of bacteria. Methods Enzymol 294: 458-482.
22. Cordero-Morales JF, Cuello LG, Zhao Y, Jogini V, Cortes DM, et al. (2006) Molecular determinants of gating at the potassium-channel selectivity filter. Nat Struct Mol Biol 13: 311-318.
23. Hoshi T, Zagotta WN, Aldrich RW (1990) Biophysical and molecular mechanisms of Shaker potassium channel inactivation. Science 250: 533-538.
24. Zagotta WN, Hoshi T, Aldrich RW (1990) Restoration of inactivation in mutants of Shaker potassium channels by a peptide derived from ShB. Science 250: 568-571.
25. MacKinnon R, Aldrich RW, Lee AW (1993) Functional stoichiometry of Shaker potassium channel inactivation. Science 262: 757-759.
26. Kreusch A, Pfaffinger PJ, Stevens CF, Choe S (1998) Crystal structure of the tetramerization domain of the Shaker potassium channel. Nature 392: 945-948.
27. + channels. Nat Struct Mol Biol 6: 38-43.
28. Choe S, Kreusch A, Pfaffinger PJ (1999) Towards the three-dimensional structure of voltage-gated potassium channels. Trends Biochem Sci 24: 345-349.
29. Cushman SJ, Nanao MH, Jahng AW, DeRubeis D, Choe S, et al. (2000) Voltage dependent activation of potassium channels is coupled to T1 domain structure. Nat Struct Mol Biol 7: 403-407.
30. + channels. Science 289: 123-127.
31. Zhou M, Morais-Cabral JH, Mann S, MacKinnon R (2001) Potassium channel receptor site for the inactivation gate and quaternary amine inhibitors. Nature 411: 657-661.
32. Long SB, Campbell EB, MacKinnon R (2005) Crystal structure of a mammalian voltage-dependent shaker family K+ channel. Science 309: 897-903.
33. Baker KA, Hilty C, Peti W, Prince A, Pfaffinger PJ, et al. (2006) NMR-derived dynamic aspects of N-type inactivation of a Kv channel suggest a aransientinteraction with the T1 domain. Biochemistry 45: 1663-1672.
34. Kefala G, Kwiatkowski W, Esquivies L, Maslennikov I, Choe S (2007) Application of Mistic to improving the expression and membrane integration of histidine kinase receptors from Escherichia coli. J Struct Funct Genomics 8: 167-172.
35. + channel using Escherichia coli as a host. J Biol Chem 282: 24294-24301.
36. + conduit in vivo. EMBO J 22: 4049-4058.