The active spatial organization of the β-globin locus requires the transcription factor EKLF

Genes and Development

Volumn 18, Issue 20, 2004, Pages 2485-2490

  Drissen, Roy ^a   Palstra, Robert Jan ^a   Gillemans, Nynke ^a   Splinter, Erik ^a   Grosveld, Frank ^a   Philipsen, Sjaak ^a   De Laat, Wouter ^a  

^a ERASMUS MEDICAL CENTER   (Netherlands)

Author keywords

ACH; Chromatin; EKLF; Globin; Spatial organization; Transcription factors

Indexed keywords

BETA GLOBIN; ERYTHROID KRUPPEL LIKE FACTOR;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CHROMATIN; CONTROLLED STUDY; FETUS; GENE EXPRESSION; GENE LOCUS; GENETIC TRANSCRIPTION; MOUSE; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; CHROMATIN; CROSS-LINKING REAGENTS; DNA; DNA PRIMERS; DNA, COMPLEMENTARY; DNA-BINDING PROTEINS; FORMALDEHYDE; GENE EXPRESSION REGULATION; GLOBINS; KRUPPEL-LIKE TRANSCRIPTION FACTORS; LIVER; MICE; MICE, MUTANT STRAINS; POLYMERASE CHAIN REACTION; RESTRICTION MAPPING; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSCRIPTION FACTORS;

ANIMALIA;

EID: 5444255213     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.317004     Document Type: Article

References (33)

1. Armstrong, J.A., Bieker, J.J., and Emerson, B.M. 1998. A SWI/SNF-related chromatin remodeling complex, E-RCI, is required for tissue-specific transcriptional regulation by EKLF in vitro. Cell 95: 93-104.
2. Bender, M.A., Bulger, M., Close, J., and Groudine, M. 2000. β-globin gene switching and DNase I sensitivity of the endogenous β-globin locus in mice do not require the locus control region. Mol Cell 5:387-393.
3. Bieker, J.J. 2001. Kruppel-like factors: Three fingers in many pies. J. Biol. Chem. 276: 34355-34358.
4. Carter, D., Chakalova, L., Osborne, C.S., Dai, Y.F., and Fraser, P. 2002. Long-range chromatin regulatory interactions in vivo. Nat. Genet. 32: 623-626.
5. De Laat, W. and Grosveld, F. 2003. Spatial organization of gene expression: The Active Chromatin Hub. Chromosome Res. 5: 447-459.
6. Dekker, J., Rippe, K., Dekker, M., and Kleckner, N. 2002. Capturing chromosome conformation. Science 295: 1306-1311.
7. Dolznig, H., Boulme, F., Stangl, K., Deiner, E.M., Mikulits, W., Beug, H., and Mullner, E.W. 2001. Establishment of normal, terminally differentiating mouse erythroid progenitors: Molecular characterization by cDNA arrays. FASEB. J. 15: 1442-1444.
8. Ellis, J., Talbot, D., Dillon, N., and Grosveld, F. 1993. Synthetic human β-globin 5′HS2 constructs function as locus control regions only in multicopy transgene concatamers. EMBO J. 12: 127-134.
9. Ellis, J., Tan-Un, K.C., Harper, A., Michalovich, D., Yannoutsos, N., Philipsen, S., and Grosveld, F. 1996. A dominant chromatin-opening activity in 5′ hypersensitive site 3 of the human β-globin locus control region. EMBO J. 15: 562-568.
10. Fiering, S., Epner, E., Robinson, K., Zhuang, Y., Telling, A., Hu, M., Martin, D.I., Enver, T., Ley, T.J., and Groudine, M. 1995. Targeted deletion of 5′HS2 of the murine β-globin LCR reveals that it is not essential for proper regulation of the β-globin locus. Genes & Dev. 9: 2203-2213.
11. Flint, J., Tufarelli, C., Peden, J., Clark, K., Daniels, R.J., Hardison, R., Miller, W., Philipsen, S., Tan-Un, K.C., McMorrow, T., et al. 2001. Comparative genome analysis delimits a chromosomal domain and identifies key regulatory elements in the α globin cluster. Hum. Mol. Genet. 10:371-382.
12. Fraser, P., Pruzina, S., Antoniou, M., and Grosveld, F. 1993. Each hypersensitive site of the human β-globin locus control region confers a different developmental pattern of expression on the globin genes. Genes & Dev. 7: 106-113.
13. Gillemans, N., Tewari, R., Lindeboom, F., Rottier, R., de Wit, T., Wijgerde, M., Grosveld, F., and Philipsen, S. 1998. Altered DNA-binding specificity mutants of EKLF and Sp1 show that EKLF is an activator of the β-globin locus control region in vivo. Genes & Dev. 12: 2863-2873.
14. Grosveld, F., van Assendelft, G.B., Greaves, D.R., and Kollias, G. 1987. Position-independent, high-level expression of the human β-globin gene in transgenic mice. Cell 51: 975-985.
15. Hug, B.A., Wesselschmidt, R.L., Fiering, S., Bender, M.A., Epner, E., Groudine, M., and Ley, T.J. 1996. Analysis of mice containing a targeted deletion of β-globin locus control region 5′ hypersensitive site 3. Mol. Cell. Biol. 16: 2906-2912.
16. Kollias, G., Wrighton, N., Hurst, J., and Grosveld, F. 1986. Regulated expression of human A γ-, β-, and hybrid γ β-globin genes in transgenic mice: Manipulation of the developmental expression patterns. Cell 46: 89-94.
17. -/- adult erythrocytes, due to a deficiency of β-globin chains, is ameliorated by human γ-globin chains. Blood 90: 1291-1299.
18. Littlewood, T.D., Hancock, D.C., Danielian, P.S., Parker, M.G., and Evan, G.I. 1995. A modified oestrogen receptor ligand-binding domain as an improved switch for the regulation of heterologous proteins. Nucleic Acids Res. 23: 1686-1690.
19. Miller, I.J. and Bieker, J.J. 1993. A novel, erythroid cell-specific murine transcription factor that binds to the CACCC element and is related to the Kruppel family of nuclear proteins. Mol Cell. Biol. 13: 2776-2786.
20. Needham, M., Gooding, C., Hudson, K., Antoniou, M., Grosveld, F., and Hollis, M. 1992. LCR/MEL: A versatile system for high-level expression of heterologous proteins in erythroid cells. Nucleic Acids Res. 20: 997-1003.
21. Nuez, B., Michalovich, D., Bygrave, A., Ploemacher, R., and Grosveld, F. 1995. Defective hematopoiesis in fetal liver resulting from inactivation of the EKLF gene. Nature 375: 316-318.
22. Palstra, R.J., Tolhuis, B., Splinter, E., Nijmeijer, R., Grosveld, F., and de Laat, W. 2003. The β-globin nuclear compartment in development and erythroid differentiation. Nat. Genet. 35: 190-194.
23. Patrinos, G.P., de Krom, M., de Boer, E., Langeveld, A., Imam, A.M., Strouboulis, J., de Laat, W., and Grosveld, F.G. 2004. Multiple interactions between regulatory regions are required to stabilize an active chromatin hub. Genes & Dev. 18: 1495-1509.
24. Perkins, A. 1999. Erythroid Kruppel like factor: From fishing expedition to gourmet meal. Int. J. Biochem. Cell Biol 31: 1175-1192.
25. Perkins, A.C., Sharpe, A.H., and Orkin, S.H. 1995. Lethal β-thalassaemia in mice lacking the erythroid CACCC-transcription factor EKLF. Nature 375: 318-322.
26. Splinter, E., Grosveld, F., and de Laat, W. 2004. 3C technology: Analyzing the spatial organization of genomic loci in vivo. Methods Enzymol. 375: 493-507.
27. Tewari, R., Gillemans, N., Wijgerde, M., Nuez, B., von Lindern, M., Grosveld, F., and Philipsen, S. 1998. Erythroid Kruppel-like factor (EKLF) is active in primitive and definitive erythroid cells and is required for the function of 5′HSS of the β-globin locus control region. EMBO J. 17: 2334-2341.
28. Tolhuis, B., Palstra, R.J., Splinter, E., Grosveld, F., and de Laat, W. 2002. Looping and interaction between hypersensitive sites in the active β-globin locus. Mol Cell 10: 1453-1465.
29. Trimborn, T., Gribnau, J., Grosveld, F., and Fraser, P. 1999. Mechanisms of developmental control of transcription in the murine α- and β-globin loci. Genes & Dev. 13: 112-124.
30. Van de Corput, M.P. and Grosveld, F.G. 2001. Fluorescence in situ hybridization analysis of transcript dynamics in cells. Methods 25: 111-118.
31. Wall, L., deBoer, E., and Grosveld, F. 1988. The human β-globin gene 3′ enhancer contains multiple binding sites for an erythroid-specific protein. Genes & Dev. 2: 1089-1100.
32. Wijgerde, M., Grosveld, F., and Fraser, P. 1995. Transcription complex stability and chromatin dynamics in vivo. Nature 377: 209-213.
33. Wijgerde, M., Gribnau, J., Trimborn, T., Nuez, B., Philipsen, S., Grosveld, F., and Fraser, P. 1996. The role of EKLF in human β-globin gene competition. Genes & Dev. 10: 2894-2902.