Deciphering kas operon locus in Mycobacterium aurum and genesis of a recombinant strain for rational-based drug screening

Journal of Applied Microbiology

Volumn 105, Issue 5, 2008, Pages 1703-1710

  Gupta, N ^a   Singh, B N ^a  

^a CENTRAL DRUG RESEARCH INSTITUTE   (India)

Author keywords

FAS II system; Isoniazid; kas operon; Mycobacterium aurum

Indexed keywords

CLONING; GENE EXPRESSION;

FATTY ACID SYNTHASE; FATTY ACID SYNTHASE TYPE II SYSTEM; ISONIAZID; KAS OPERON; M. TUBERCULOSIS; MYCOBACTERIA AURUM; RECOMBINANT STRAINS; REPORTER GENE; REPORTER-GENE EXPRESSION; TYPE II;

FATTY ACIDS;

ANTIMYCOBACTERIAL AGENT; FATTY ACID SYNTHASE; FATTY ACID SYNTHASE INHIBITOR;

ANTIMICROBIAL ACTIVITY; BACTERIUM; DRUG; ENZYME ACTIVITY; FATTY ACID; GENE EXPRESSION; INHIBITOR; RECOMBINATION;

ANTIBACTERIAL ACTIVITY; ANTIBIOTIC THERAPY; ARTICLE; BACTERIAL STRAIN; CLONING; DRUG SCREENING; GENE EXPRESSION; MYCOBACTERIUM AURUM; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; REPORTER GENE; SEQUENCE ANALYSIS; UPREGULATION;

MYCOBACTERIUM AURUM; MYCOBACTERIUM TUBERCULOSIS;

EID: 54049144269     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2008.03888.x     Document Type: Article

References (18)

1. Alland, D., Steyn, A.J., Weisbrod, T., Aldrich, K. Jacobs, W.R. Jr. (2000) Characterization of the Mycobacterium tuberculosis iniBAC promoter, a promoter that responds to cell wall biosynthesis inhibition. J Bacteriol 182, 1802 1811.
2. Bardou, F., Quemard, A., Dupont, M.A., Horn, C., Marchal, C. Daffe, M. (1996) Effects of isoniazid on ultrastructure of Mycobacterium aurum and Mycobacterium tuberculosis and on production of secreted proteins. Antimicrob Agents Chemother 40, 2459 2467.
3. Betts, C.J., McLaren, A., Lennon, M.G., Kelly, F.M., Lukey, P.T., Blackmore, S.J. Duncan, K. (2003) Signature gene expression profiles discriminate between isoniazid, thiolactomycin and triclosan treated Mycobacterium tuberculosis. Antimicrob Agents Chemother 47, 2903 2913.
4. Chung, G.A.C., Aktar, Z., Jackson, S. Duncan, K. (1995) High-throughput screen for detecting antimycobacterial agents. Antimicrob Agents Chemother 39, 2235 2238.
5. Cole, S.T., Brosch, R., Parkhill, J., Garnier, T., Churcher, C., Harris, D., Gordon, S.V., Eiglmeier, K. et al. (1998) Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393, 537 544.
6. Deb, D.K., Srivastava, K.K., Srivastava, R. Srivastava, B.S. (2000) Bioluminescent Mycobacterium aurum expressing firefly luciferase for rapid and high throughput screening of antimycobacterial drugs in vitro and in infected macrophages. Biochem Biophys Res Commun 279, 457 461.
7. Fischer, H.P., Brunner, N.A., Wieland, B., Paquette, J., Macko, L., Ziegelbauer, K. Freiberg, C. (2004) Identification of antibiotic stress-inducible promoters: a systematic approach to novel pathway-specific reporter assays for antibacterial drug discovery. Genome Res 14, 90 98.
8. Heath, R.J., White, S.W. Rock, C.O. (2002) Inhibitors of fatty acid synthesis as antimicrobial chemotherapeutics. Appl Microbiol Biotechnol 58, 695 703.
9. Kremer, L., Dover, L.G., Morbidoni, H.R., Vilcheze, C., Maughan, W.N., Baulard, A., Tu, S.-C., Honore, N. et al. (2003) Inhibition of InhA activity, but not KasA activity, induces formation of a KasA-containing complex in Mycobacteria. J Biol Chem 278, 20547 20554.
10. Michele, T.M., Ko, C. Bishai, W.R. (1999) Exposure to antibiotics induces expression of the Mycobacterium tuberculosis sigF gene: Implications for chemotherapy against Mycobacterial persistors. Antimicrob Agents Chemother 43, 218 225.
11. Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor, NY : Cold Spring Harbor Laboratory.
12. Payne, D.J., Warren, P.V., Holmes, D.J., Ji, Y. Lonsdale, J.T. (2001) Bacterial fatty-acid biosynthesis: a genomics driven target for antibacterial drug discovery. Drug Discov Today 6, 537 544.
13. Quemard, A., Laneele, G. Lacave, C. (1992) Mycolic acid synthesis: a target for ethionamide in Mycobacteria. Antimicrob Agents Chemother 36, 1316 1321.
14. Slayden, R.A. Barry, C.E. III. (2002) The role of KasA and KasB in the biosynthesis of meromycolic acids and isoniazid resistance in Mycobacterium tuberculosis. Tuberculosis (Edinburgh) 82, 149 160.
15. Slayden, R.A., Lee, R.E. Barry, C.E. III. (2000) Isoniazid affects multiple components of the type II fatty acid synthase system of Mycobacterium tuberculosis. Mol Microbiol 38, 514 525.
16. Vilcheze, C., Wang, F., Arai, M., Hazbon, M.H., Colangeli, R., Kremer, L., Weisbrod, T.R., Alland, D. et al. (2006) Transfer of a point mutation in Mycobacterium tuberculosis inhA resolves the target of isoniazid. Nature Med 12, 1027 1029.
17. +. Biochem J 318, 451 457.
18. Wilson, M., DeRisi, J., Kristensen, H.H., Imboden, P., Rane, S., Brown, P.O. Schoolnik, G.K. (1999) Exploring drug-induced alterations in gene expression in Mycobacterium tuberculosis by microarray hybridization. Proc Natl Acad Sci USA 96, 12833 12838.