RXP-E: A connexin43-binding peptide that prevents action potential propagation block

Circulation Research

Volumn 103, Issue 5, 2008, Pages 519-526

  Lewandowski, Rebecca ^a   Procida, Kristina ^b   Vaidyanathan, Ravi ^a   Coombs, Wanda ^a   Jalife, José ^c   Nielsen, Morten S ^b   Taffet, Steven M ^a   Delmar, Mario ^c  

^a SUNY UPSTATE MEDICAL UNIVERSITY   (United States)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

Connexin43; Cx43CT; Gap junctions; Particle receptor interaction; Rotigaptide

Indexed keywords

BINDING PROTEIN; CONNEXIN 43; HEPTANOL; INWARDLY RECTIFYING POTASSIUM CHANNEL SUBUNIT KIR2.1; RXP E; UNCLASSIFIED DRUG; ACID; CARRIER PROTEIN; INWARDLY RECTIFYING POTASSIUM CHANNEL; KCNJ4 PROTEIN, RAT; KIR2.1 CHANNEL; RXP E PEPTIDE; RXP-E PEPTIDE; SODIUM CHANNEL; VOLTAGE GATED NA+ CHANNEL NA(V)1.5A; VOLTAGE-GATED NA+ CHANNEL NA(V)1.5A;

ACIDIFICATION; ACTION POTENTIAL; ANIMAL CELL; ANIMAL CELL CULTURE; ANIMAL EXPERIMENT; ARTICLE; CARBOXY TERMINAL SEQUENCE; ELECTROPHYSIOLOGY; GAP JUNCTION; HEART ARRHYTHMIA; HEART MUSCLE CELL; HEART VENTRICLE; ION CURRENT; MYOFIBROBLAST; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; RAT; SODIUM CURRENT; ANIMAL; CELL CULTURE; CELL JUNCTION; CYTOLOGY; DRUG DESIGN; DRUG EFFECT; METABOLISM; PH; PHYSIOLOGY; PROTEIN BINDING; SYNTHESIS; TRANSPORT AT THE CELLULAR LEVEL;

ACIDS; ACTION POTENTIALS; ANIMALS; BIOLOGICAL TRANSPORT; CARRIER PROTEINS; CELLS, CULTURED; CONNEXIN 43; DRUG DESIGN; GAP JUNCTIONS; HEPTANOL; HYDROGEN-ION CONCENTRATION; MYOCYTES, CARDIAC; PATCH-CLAMP TECHNIQUES; POTASSIUM CHANNELS, INWARDLY RECTIFYING; PROTEIN BINDING; RATS; SODIUM CHANNELS;

EID: 54049085064     PISSN: 00097330     EISSN: 15244571     Source Type: Journal    
DOI: 10.1161/CIRCRESAHA.108.179069     Document Type: Article

References (39)

1. Morley GE, Taffet SM, Delmar M. Intramolecular interactions mediate pH regulation of connexin43 channels. Biophys J. 1996;70:1294-1302.
2. Ek-Vitorin JF, Calero G, Morley GE, Coombs W, Taffet SM, Delmar M. PH regulation of connexin43: molecular analysis of the gating particle. Biophys J. 1996;71:1273-1284.
3. Maass K, Ghanem A, Kim JS, Saathoff M, Urschel S, Kirfel G, Grümmer R, Kretz M, Lewalter T, Tiemann K, Winterhager E, Herzog V, Willecke K. Defective epidermal barrier in neonatal mice lacking the C-terminal region of connexin43. Mol Biol Cell. 2004;15:4597-4608.
4. Calero G, Kanemitsu M, Taffet SM, Lau AF, Delmar M. A 17mer peptide interferes with acidification-induced uncoupling of connexin43. Circ Res. 1998;82:929-935.
5. Seki A, Coombs W, Taffet SM, Delmar M. Loss of electrical communication, but not plaque formation, after mutations in the cytoplasmic loop of connexin43.see comment. Heart Rhythm. 2004;1:227-233.
6. Shibayama J, Lewandowski R, Kieken F, Coombs W, Shah S, Sorgen PL, Taffet SM, Delmar M. Identification of a novel peptide that interferes with the chemical regulation of connexin43. Circ Res. 2006;98: 1365-1372.
7. Srinivas M, Duffy HS, Delmar M, Spray DC. Prospects for pharmacological targeting of gap junction channels. In: Zipes DZ, Jalife J, eds. Cardiac Electrophysiology: From Cell to Bedside. 4th ed. Philadelphia: Saunders; 2004:158-167.
8. Herve JC, Dhein S. Pharmacology of cardiovascular gap junctions. Adv Cardiol. 2006;42:107-131.
9. Eloff BC, Gilat E, Wan X, Rosenbaum DS. Pharmacological modulation of cardiac gap junctions to enhance cardiac conduction: evidence supporting a novel target for antiarrhythmic therapy. Circulation. 2003;108: 3157-3163.
10. Haugan K, Petersen JS. Gap junction modifying antiarrhythmic peptides: therapeutic potential in atrial fibrillation. Drugs Future. 2007;32: 245-260.
11. Oxford EM, Musa H, Maass K, Coombs W, Taffet SM, Delmar M. Connexin43 remodeling caused by inhibition of plakophilin-2 expression in cardiac cells. Circ Res. 2007;101:703-711.
12. Gaudesius G, Miragoli M, Thomas SP, Rohr S. Coupling of cardiac electrical activity over extended distances by fibroblasts of cardiac origin. Circ Res. 2003;93:421-428.
13. Rohr S, Fluckiger-Labrada R, Kucera JP. Photolithographically defined deposition of attachment factors as a versatile method for patterning the growth of different cell types in culture. Pflugers Arch. 2003;446: 125-132.
14. Kucera JP, Heuschkel MO, Renaud P, Rohr S. Power-law behavior of beat-rate variability in monolayer cultures of neonatal rat ventricular myocytes. Circ Res. 2000;86:1140-1145.
15. Kim D, Jeon C, Kim JH, Kim MS, Yoon CH, Choi IS, Kim SH, Bae YS. Cytoplasmic transduction peptide (CTP): new approach for the delivery of biomolecules into cytoplasm in vitro and in vivo. Exp Cell Res. 2006;312:1277-1288.
16. Moreno AP. Biophysical properties of homomeric and heteromultimeric channels formed by cardiac connexins. Cardiovasc Res. 2004;62: 276-286.
17. Seki A, Duffy HS, Coombs W, Spray DC, Taffet SM, Delmar M. Modifications in the biophysical properties of connexin43 channels by a peptide of the cytoplasmic loop region. Circ Res. 2004;95:e22-e28.
18. Munoz V, Vaidyanathan R, Tolkacheva EG, Dhamoon AS, Taffet SM, Anumonwo JM. Kir2.3 isoform confers pH sensitivity to heteromeric Kir2.1/Kir2.3 channels in HEK293 cells. Heart Rhythm. 2007;4: 487-496.
19. Dhamoon AS, Pandit SV, Sarmast F, Parisian KR, Guha P, Li Y, Bagwe S, Taffet SM, Anumonwo JM. Unique Kir2.x properties determine regional and species differences in the cardiac inward rectifier K + current. Circ Res. 2004;94:1332-1339.
20. Mun'oz V, Grzeda KR, Desplantez T, Pandit SV, Mironov S, Taffet SM, Rohr S, Kleber AG, Jalife J. Adenoviral expression of IKs contributes to wavebreak and fibrillatory conduction in neonatal rat ventricular cardio-myocyte monolayers. Circ Res. 2007;101:475-483.
21. Nelson WL, Makielski JC. Block of sodium current by heptanol in voltage-clamped canine cardiac purkinje cells. Circ Res. 1991;68: 977-983.
22. Frankel AD, Pabo CO. Cellular uptake of the tat protein from human immunodeficiency virus. Cell. 1988;55:1189-1193.
23. Green M, Loewenstein PM. Autonomous functional domains of chemically synthesized human immunodeficiency virus tat trans-activator protein. Cell. 1988;55:1179-1188.
24. Wadia JS, Stan RV, Dowdy SF. Transducible TAT-HA fusogenic peptide enhances escape of TAT-fusion proteins after lipid raft macropinocytosis. Nat Med. 2004;10:310-315.
25. Miragoli M, Gaudesius G, Rohr S. Electrotonic modulation of cardiac impulse conduction by myofibroblasts. Circ Res. 2006;98:801-810.
26. Miragoli M, Salvarani N, Rohr S. Myofibroblasts induce ectopic activity in cardiac tissue. Circ Res. 2007;101:755-758.
27. Kohl P, Camelliti P. Cardiac myocyte-nonmyocyte electrotonic coupling: implications for ventricular arrhythmogenesis. Heart Rhythm. 2007;4: 233-235.
28. Darrow BJ, Fast VG, Kleber AG, Beyer EC, Saffitz JE. Functional and structural assessment of intercellular communication. increased conduction velocity and enhanced connexin expression in dibutyryl cAMP-treated cultured cardiac myocytes. Circ Res. 1996;79:174-183.
29. Rohr S, Kucera JP, Fast VG, Kleber AG. Paradoxical improvement of impulse conduction in cardiac tissue by partial cellular uncoupling. Science. 1997;275:841-844.
30. Kleber AG, Rudy Y. Basic mechanisms of cardiac impulse propagation and associated arrhythmias. Physiol Rev. 2004;84:431-488.
31. Severs NJ, Coppen SR, Dupont E, Yeh HI, Ko YS, Matsushita T. Gap junction alterations in human cardiac disease. Cardiovasc Res. 2004;62: 368-377.
32. De Mello WC. Cyclic AMP and junctional communication viewed through a multibiophysical approach. In: Peracchia C, ed. Biophysics of Gap Junction Channels. Boca Raton, Fla: CRC Press; 1991:229-239.
33. De Mello WC. Effect of intracellular injection of calcium and strontium on cell communication in heart. J Physiol. 1975;250:231-245.
34. Burt JM, Spray DC. Inotropic agents modulate gap junctional conductance between cardiac myocytes. Am J Physiol. 1988;254: H1206-H1210.
35. Doble BW, Chen Y, Bosc DG, Litchfield DW, Kardami E. Fibroblast growth factor-2 decreases metabolic coupling and stimulates phosphorylation as well as masking of connexin43 epitopes in cardiac myocytes. Circ Res. 1996;79:647-658.
36. Pimentel RC, Yamada KA, Kleber AG, Saffitz JE. Autocrine regulation of myocyte Cx43 expression by VEGF. Circ Res. 2002;90:671-677.
37. Dhein S, Polontchouk L, Salameh A, Haefliger JA. Pharmacological modulation and differential regulation of the cardiac gap junction proteins connexin 43 and connexin 40. Biol Cell. 2002;94:409-422.
38. Muller DJ, Hand GM, Engel A, Sosinsky GE. Conformational changes in surface structures of isolated connexin 26 gap junctions. EMBO J. 2002; 21:3598-3607.
39. Dhein S, Weng S, Grover R, Tudyka T, Gottwald M, Schaefer T, Polontchouk L. Protein kinase C alpha mediates the effect of antiarrhythmic peptide on gap junction conductance. Cell Commun Adhes. 2001;8:257-264.