A 17mer peptide interferes with acidification-induced uncoupling of connexin43

Circulation Research

Volumn 82, Issue 9, 1998, Pages 929-935

  Calero, Guillermo ^a   Kanemitsu, Martha ^b   Taffet, Steven M ^a   Lau, Alan F ^c   Delmar, Mario ^a  

^a SUNY UPSTATE MEDICAL UNIVERSITY   (United States)

^b SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

^c UNIVERSITY OF HAWAII CANCER CENTER   (United States)

Author keywords

Cardiac arrhythmia; Connexin; Gap junction; Peptide; Proline rich peptide; Xenopus oocyte

Indexed keywords

CONNEXIN 43; UNCOUPLING PROTEIN;

ACIDIFICATION; AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL COMMUNICATION; CHANNEL GATING; DNA FLANKING REGION; GAP JUNCTION; HUMAN CELL; NONHUMAN; OOCYTE; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN DOMAIN; VOLTAGE CLAMP; XENOPUS;

EID: 0032543289     PISSN: 00097330     EISSN: None     Source Type: Journal    
DOI: 10.1161/01.RES.82.9.929     Document Type: Article

References (33)

1. Reaume AG, de Sousa PA, Kulkarni S, Langille BL, Zhu D, Davies TC, Juneja SC, Kidder GM, Rossant J. Cardiac malformations in neonatal mice lacking connexin43. Science. 1995;267:1831-1834.
2. Ewart JL, Cohen MF, Meyer RA, Huang GY, Wessels A, Gourdie RG, Chin AJ, Park SJ, Lazatin BO, Villabon S, Lo CW. Heart and neural tube defects in transgenic mice overexpressing the Cx43 gap junction gene. Development. 1997;124:1281-1292.
3. Kleber AG, Riegger CB, Janse MJ. Electrical uncoupling and increase of extracellular resistance after induction of ischemia in isolated, arterially perfused rabbit papillary muscle. Circ Res. 1987;61:271-279.
4. Guerrero PA, Schuessler RB, Davis LM, Beyer EC, Johnson CM, Yamada KA, Saffitz JE. Slow ventricular conduction in mice heterozygous for a connexin43 null mutation. J Clin Invest. 1997;99:1991-1998.
5. Delmar M, Liu S, Morley GE, Ek JF, Anumonwo JMB, Taffet SM. Toward a molecular model for the pH regulation of intercellular communication in the heart. In: Zipes DP, Jalife J, eds. Cardiac Electrophysiology: From Cell to Bedside. Philadelphia, Pa: WB Saunders Co; 1995:135-143.
6. Bruzzone R, White TW, Paul DL. Connections with connexins: the molecular basis of direct intercellular signaling. Eur J Biochem. 1996; 238:1-27.
7. Goodenough DA, Goliger JA, Paul DL. Connexins, connexons and intercellular communication. Annu Rev Biochem. 1996;65:475-502.
8. Lau AF, Kurata WE, Kanemitsu MY, Loo LWM, Warn-Cramer BJ, Eckhart W, Lampe PD. Regulation of connexin43 function by activated tyrosine protein kinases. J Bioenerg Biomembr. 1996;28:359-368.
9. Yeager M, Nicholson BJ. Structure of gap junction intercellular channels. Curr Opin Struct Biol. 1996;6:183-192.
10. Cascio WE, Johnson TA, Gettes LS. Electrophysiologic changes in ischemic ventricular myocardium, I: influence of ionic, metabolic and energy changes. J Cardiovasc Electrophysiol. 1995;6:1039-1062.
11. Janse MJ, Opthof T. Mechanisms of ischemia-induced arrhythmias. In: Zipes DP, Jalife J, eds. Cardiac Electrophysiology: From Cell to Bedside. Philadelphia, Pa: WB Saunders Co; 1995:489-496.
12. Janse MJ, Wit AL. Electrophysiological mechanisms of ventricular arrhythmias resulting from myocardial ischemia and infarction. Physiol Rev. 1989;69:1049-1169.
13. Liu S, Taffet SM, Stoner L, Delmar M, Vallano ML, Jalife J. A structural basis for the unequal sensitivity of the major cardiac and liver gap junctions to intracellular acidification: the carboxyl terminal length. Biophys J. 1993;64:1422-1433.
14. Morley GE, Taffet SM, Delmar M. Intramolecular interactions mediate the pH regulation of connexin43. Biophys J. 1996;70:1294-1302.
15. Armstrong CM, Bezanilla F. Inactivation of the sodium channel, II: gating current experiments. J Gen Physiol. 1977;70:567-590.
16. Hoshi T, Zagotta WN, Aldrich RW. Biophysical and molecular mechanisms of Shaker potassium channel inactivation. Science. 1990;250:533-538.
17. Ek-Vitorin JF, Calero G, Morley GE, Coombs W, Taffet SM, Delmar M. pH regulation of connexin43: molecular analysis of the gating particle. Biophys J. 1996;71:1273-1284.
18. Adzhubei AA, Sternberg MJE. Left-handed polyproline II helices commonly occur in globular proteins. J Mol Biol. 1993;229:472-493.
19. Williamson MP. The structure and function of proline-rich regions in proteins. Biochem J. 1994;297:249-260.
20. Cohen GB, Ren R, Baltimore D. Modular binding domains in signal transduction proteins. Cell. 1995;80:237-248.
21. Yu H, Chen JK, Feng S, Dalgamo DC, Brauer AW, Schreiber SL. Structural basis for the binding of proline-rich peptides to SH3 domains. Cell. 1994;76:933-945.
22. Wam-Cramer BJ, Lampe PD, Kurata WE, Kanemitsu MY, Loo LWM, Eckhart W, Lau AF. Characterization of the mitogen-activated protein kinase phosphorylation sites on the connexin-43 gap junction protein. J Biol Chem. 1996;271:3779-3786.
23. Kanemitsu M, Loo Y, Simon LWM, Lau AF, Eckhart W. Tyrosine phosphorylation of connexin43 by vSrc is mediated by SH2 and SH3 domain interactions. J Biol Chem. 1997;272:22824-22831.
24. Morley GE, Ek-Vitorin JF, Taffet SM, Delmar M. Structure of connexin43 and its regulation by pHi. J Cardiovasc Electrophysiol. 1997;8:939-951.
25. Delmar M, Morley GE, Taffet SM. Molecular analysis of the pH regulation of the cardiac gap junction protein connexin43. In: Spooner PM, Joyner RW, Jalife J, eds. Discontinuous Conduction in the Heart. Armonk, NY: Futura Publishing Co; 1997.
26. Ek JF, Delmar M, Perzova R, Taffet SM. Role of Histidine 95 in pH gating of the cardiac gap junction protein connexin43. Circ Res. 1994;74:1058-1064.
27. Sparks AB, Rider JE, Hoffman NG, Fowlkes DM, Quilliam LA, Kay BK. Distinct ligand preferences of src homology 3 domains from src, yes, ab1, cortactin, p53bp2, pic, crk and grb2. Proc Natl Acad Sci U S A. 1996;93:1540-1544.
28. Peracchia C, Wang X, Li L, Peracchia LL. Inhibition of calmodulin expression prevents low-pH-induced gap junction uncoupling in Xenopus oocytes. Pflugers Arch. 1996;431:379-387.
29. Nicholson BJ, Zhou L, Cao F, Zhu H, Chen Y. Diverse molecular mechanisms of gap junction channel gating. In: Werner R, ed. Gap Junctions. Amsterdam, Netherlands: IOS Press; 1998:3-7.
30. Valiunas V, Bukauskas F, Weingart R. Conductances and selective permeability of connexin43 gap junction channels examined in neonatal rat heart cells. Circ Res. 1997;80:708-719.
31. Niggli E, Rudisuli A, Maurer P, Weingart R. Effect of general anesthetics on current flow across membranes in guinea pig myocytes. Am J Physiol. 1989;256:C273-C281.
32. Muller A, Gottwald M, Tudyka T, Linke W, Klaus W, Dhein S. Increase in gap junction conductance by an antiarrhythmic peptide. Eur J Pharmacol. 1997;327:65-72.
33. Wilders R, Jongsma HJ. Limitations of the dual voltage clamp method in assaying conductance and kinetics of gap junction channels. Biophys J. 1992;63:942-953.