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Volumn 275, Issue 5301, 1997, Pages 841-844

Paradoxical improvement of impulse conduction in cardiac tissue by partial cellular uncoupling

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; HEART ARRHYTHMIA; HEART CONDUCTION; HEART ELECTROPHYSIOLOGY; MEMBRANE CURRENT; PRIORITY JOURNAL;

EID: 0031037674     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.275.5301.841     Document Type: Article
Times cited : (266)

References (19)
  • 8
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    • The patterns used in the experiments consisted of cell strands (25 to 70 μm wide and 1.8 mm long) connected to a large rectangular cell monolayer (2.2 by 2.2 mm). The preparations were mounted on an in verted microscope equipped for epifluorescence and were superfused with Hank's balanced salt solution (pH 7.40 at 36°C)
    • Geometrically defined patterns of cultured neonatal rat ventricular myocytes were obtained by means of a previously described method [S. Rohr, D. M. Schölly, A. G. Kléber, Circ. Res. 68, 114 (1991)]. The patterns used in the experiments consisted of cell strands (25 to 70 μm wide and 1.8 mm long) connected to a large rectangular cell monolayer (2.2 by 2.2 mm). The preparations were mounted on an in verted microscope equipped for epifluorescence and were superfused with Hank's balanced salt solution (pH 7.40 at 36°C).
    • (1991) Circ. Res. , vol.68 , pp. 114
    • Rohr, S.1    Schölly, D.M.2    Kléber, A.G.3
  • 9
    • 0029102366 scopus 로고
    • The preparations were stained with the voltage-sensitive dye di-8-ANEPPS (Molecular Probes, Eugene, OR), and propagating action potentials were elicited at a basic cycle length of 500 ms with extracellular stimulation electrodes at a distance of >1 mm from the recording area. Changes in transmembrane voltage were monitored with a system for multiple site optical recording of transmembrane voltage with a total of 80 detectors. Optically recorded signals at a given site were normalized to signal amplitudes measured at the same site during control retrograde propagation. If an average action potential amplitude of 100 mV is assumed, the values of these normalized signals translate directly into millivolts. Activation times were determined from the time when the signal at a given site reached 50% of the full amplitude [S. Rohr, J. Cardiovasc. Electrophysiol. 6, 551 (1995)].
    • (1995) J. Cardiovasc. Electrophysiol. , vol.6 , pp. 551
    • Rohr, S.1
  • 10
    • 0028085535 scopus 로고
    • S. Rohr and B. M. Salzberg, J. Gen. Physiol. 104, 287 (1994); V. G. Fast and A. G. Kléber, Cardiovasc. Res. 29, 697 (1995).
    • (1994) J. Gen. Physiol. , vol.104 , pp. 287
    • Rohr, S.1    Salzberg, B.M.2
  • 11
    • 0029061766 scopus 로고
    • S. Rohr and B. M. Salzberg, J. Gen. Physiol. 104, 287 (1994); V. G. Fast and A. G. Kléber, Cardiovasc. Res. 29, 697 (1995).
    • (1995) Cardiovasc. Res. , vol.29 , pp. 697
    • Fast, V.G.1    Kléber, A.G.2
  • 12
    • 0024833716 scopus 로고
    • The superfusion solution consisted of normal bath medium containing 10 μM palmitoleic acid (dispersed by ultrasonication) and 20 mM sucrose, which rendered the superfusion visible through birefringence
    • The superfusion of defined regions of the preparation was performed with a system described elsewhere [J. Streit and H. D. Lux, J. Neurosci. 9, 4190 (1989)]. The superfusion solution consisted of normal bath medium containing 10 μM palmitoleic acid (dispersed by ultrasonication) and 20 mM sucrose, which rendered the superfusion visible through birefringence.
    • (1989) J. Neurosci. , vol.9 , pp. 4190
    • Streit, J.1    Lux, H.D.2
  • 16
    • 0028061307 scopus 로고
    • C. Cabo et al., Circ. Res. 75, 1014 (1994).
    • (1994) Circ. Res. , vol.75 , pp. 1014
    • Cabo, C.1
  • 19
    • 15144351918 scopus 로고    scopus 로고
    • note
    • We thank R. Flückiger Labrada for preparing the heart cell cultures and H.-R. Lüscher, J. A. S. McGuigan, E. Niggli, A. L. Obaid, and J. S. Shiner for their helpful comments on the manuscript. Supported by the Swiss National Science Foundation and the Swiss Heart Foundation.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.