BgK, a disulfide-containing sea anemone toxin blocking K + channels, can be produced in Escherichia coli cytoplasm as a functional tagged protein

Protein Expression and Purification

Volumn 38, Issue 1, 2004, Pages 69-78

  Braud, Sandrine ^a   Belin, Pascal ^a   Dassa, Janie ^a   Pardo, Liliana ^a   Mourier, Gilles ^a   Caruana, Antony ^a   Priest, Birgit T ^b   Dulski, Paula ^b   Garcia, Maria L ^b   Ménez, André ^a   Boulain, Jean Claude ^a   Gasparini, Sylvaine ^a  

^a CEA SACLAY   (France)

^b MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Cytoplasmic production; Disulfide; Potassium channel; Sea anemone toxin

Indexed keywords

COELENTERATE VENOM; POTASSIUM CHANNEL BLOCKING AGENT; RECOMBINANT PROTEIN; TOXIN BGK;

ANIMAL; ARTICLE; BIOSYNTHESIS; CHO CELL; CRICETULUS; CYTOPLASM; ELECTROPHYSIOLOGY; ESCHERICHIA COLI; HAMSTER; ISOLATION AND PURIFICATION; METABOLISM; SEA ANEMONE;

ANIMALS; CHO CELLS; CNIDARIAN VENOMS; CRICETINAE; CRICETULUS; CYTOPLASM; ELECTROPHYSIOLOGY; ESCHERICHIA COLI; POTASSIUM CHANNEL BLOCKERS; RECOMBINANT PROTEINS; SEA ANEMONES;

ACTINIARIA; ANEMONE; ESCHERICHIA COLI; SYNTHETIC CONSTRUCT;

EID: 5344279151     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.07.011     Document Type: Article

References (43)

1. G.A. Gutman, K.G. Chandy, J.P. Adelman, J. Aiyar, D.A. Bayliss, D.E. Clapham, M. Covarriubias, G.V. Desir, K. Furuichi, B. Ganetzky, M.L. Garcia, S. Grissmer, L.Y. Jan, A. Karschin, D. Kim, S. Kuperschmidt, Y. Kurachi, M. Lazdunski, F. Lesage, H.A. Lester, D. McKinnon, C.G. Nichols, I. O'Kelly, J. Robbins, G.A. Robertson, B. Rudy, M. Sanguinetti, S. Seino, W. Stuehmer, M.M. Tamkun, C.A. Vandenberg, A. Wei, H. Wulff, and R.S. Wymore International Union of Pharmacology. XLI. Compendium of voltage-gated ion channels: potassium channels Pharmacol. Rev. 55 2003 583 586
2. C. Miller An overview of the potassium channel family Genome Biol. 1 2000 REVIEWS0004
3. G.J. Kaczorowski, and M.L. Garcia Pharmacology of voltage-gated and calcium-activated potassium channels Curr. Opin. Chem. Biol. 3 1999 448 458
4. M.L. Garcia, Y. Gao, O.B. McManus, and G.J. Kaczorowski Potassium channels: from scorpion venoms to high-resolution structure Toxicon 39 2001 739 748
5. R.J. Lewis, and M.L. Garcia Therapeutic potential of venom peptides Nat. Rev. Drug Discov. 2 2003 790 802
6. J. Cotton, M. Crest, F. Bouet, N. Alessandri, M. Gola, E. Forest, E. Karlsson, O. Castaneda, A.L. Harvey, C. Vita, and A. Menez A potassium-channel toxin from the sea anemone Bunodosoma granulifera, an inhibitor for Kv1 channels. Revision of the amino acid sequence, disulfide-bridge assignment, chemical synthesis, and biological activity Eur. J. Biochem. 244 1997 192 202
7. J. Racape, A. Lecoq, R. Romi-Lebrun, J. Liu, M. Kohler, M.L. Garcia, A. Menez, and S. Gasparini Characterization of a novel radiolabeled peptide selective for a subpopulation of voltage-gated potassium channels in mammalian brain J. Biol. Chem. 277 2002 3886 3893
8. M. Dauplais, A. Lecoq, J. Song, J. Cotton, N. Jamin, B. Gilquin, C. Roumestand, C. Vita, C.L. de Medeiros, E.G. Rowan, A.L. Harvey, and A. Menez On the convergent evolution of animal toxins. Conservation of a diad of functional residues in potassium channel-blocking toxins with unrelated structures J. Biol. Chem. 272 1997 4302 4309
9. B. Gilquin, J. Racape, A. Wrisch, V. Visan, A. Lecoq, S. Grissmer, A. Menez, and S. Gasparini Structure of the BgK-Kv1.1 complex based on distance restraints identified by double mutant cycles. Molecular basis for convergent evolution of Kv1 channel blockers J. Biol. Chem. 277 2002 37406 37413
10. N. Alessandri-Haber, A. Lecoq, S. Gasparini, G. Grangier-Macmath, G. Jacquet, A.L. Harvey, C. de Medeiros, E.G. Rowan, M. Gola, A. Menez, and M. Crest Mapping the functional anatomy of BgK on Kv1.1, Kv1.2, and Kv1.3. Clues to design analogs with enhanced selectivity J. Biol. Chem. 274 1999 35653 35661
11. P.H. Bessette, F. Aslund, J. Beckwith, and G. Georgiou Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm Proc. Natl. Acad. Sci. USA 96 1999 13703 13708
12. T. Lauber, U.C. Marx, A. Schulz, P. Kreutzmann, P. Rosch, and S. Hoffmann Accurate disulfide formation in Escherichia coli: overexpression and characterization of the first domain (HF6478) of the multiple Kazal-type inhibitor LEKTI Protein Express. Purif. 22 2001 108 112
13. K. Lehmann, S. Hoffmann, P. Neudecker, M. Suhr, W.M. Becker, and P. Rosch High-yield expression in Escherichia coli, purification, and characterization of properly folded major peanut allergen Ara h 2 Protein Express. Purif. 31 2003 250 259
14. J.S. Kim, and R.T. Raines Ribonuclease S-peptide as a carrier in fusion proteins Protein Sci. 2 1993 348 356
15. B.H. Muller, C. Lamoure, M.H. Le Du, L. Cattolico, E. Lajeunesse, F. Lemaitre, A. Pearson, F. Ducancel, A. Menez, and J.C. Boulain Improving Escherichia coli alkaline phosphatase efficacy by additional mutations inside and outside the catalytic pocket Chembiochem 2 2001 517 523
16. J.C. Boulain, L. Cattolico, F. Ducancel, A. Ménez, Phosphatase alcalines bactériennes modifiées et leurs applications, French Patent Application 95.07833 (1995)
17. K. Nishihara, M. Kanemori, M. Kitagawa, H. Yanagi, and T. Yura Chaperone coexpression plasmids: differential and synergistic roles of DnaK-DnaJ-GrpE and GroEL-GroES in assisting folding of an allergen of Japanese cedar pollen, Cryj2, in Escherichia coli Appl. Environ. Microbiol. 64 1998 1694 1699
18. J. Sambrook, E.F. Fritsch, and T. Maniatis Molecular Cloning: A Laboratory Manual 1989 Cold Spring Harbor New York
19. C. Prodromou, and L.H. Pearl Recursive PCR: a novel technique for total gene synthesis Protein Eng. 5 1992 827 829
20. E. Harlow, D. Lane, Antibodies: a laboratory manual, Cold Spring Harbor, NY, 1988
21. K. Schroeder, B. Neagle, D.J. Trezise, and J. Worley Ionworks HT: a new high-throughput electrophysiology measurement platform J. Biomol. Screen. 8 2003 50 64
22. D. Missiakas, and S. Raina Protein folding in the bacterial periplasm J. Bacteriol. 179 1997 2465 2471
23. + channel blocker Biochemistry 32 1993 5692 5697
24. J.P. Imredy, C. Chen, and R. MacKinnon A snake toxin inhibitor of inward rectifier potassium channel ROMK1 Biochemistry 37 1998 14867 14874
25. J.M. Danse, E.G. Rowan, S. Gasparini, F. Ducancel, H. Vatanpour, L.C. Young, G. Poorheidari, E. Lajeunesse, P. Drevet, and R. Menez On the site by which alpha-dendrotoxin binds to voltage-dependent potassium channels: site-directed mutagenesis reveals that the lysine triplet 28-30 is not essential for binding FEBS Lett. 356 1994 153 158
26. + channel blocker purified from Androctonus australis scorpion FEBS Lett. 417 1997 123 129
27. Y.V. Korolkova, S.A. Kozlov, A.V. Lipkin, K.A. Pluzhnikov, J.K. Hadley, A.K. Filippov, D.A. Brown, K. Angelo, D. Strobaek, T. Jespersen, S.P. Olesen, B.S. Jensen, and E.V. Grishin An ERG channel inhibitor from the scorpion Buthus eupeus J. Biol. Chem. 276 2001 9868 9876
28. D. Gillet, F. Ducancel, E. Pradel, M. Leonetti, A. Menez, and J.C. Boulain Insertion of a disulfide-containing neurotoxin into E. coli alkaline phosphatase: the hybrid retains both biological activities Protein Eng. 5 1992 273 278
29. D. Gillet, E. Ezan, F. Ducancel, C. Gaillard, T. Ardouin, M. Istin, A. Menez, J.C. Boulain, and J.M. Grognet Enzyme immunoassay using a rat prolactin-alkaline phosphatase recombinant tracer Anal. Chem. 65 1993 1779 1784
30. W.A. Prinz, F. Aslund, A. Holmgren, and J. Beckwith The role of the thioredoxin and glutaredoxin pathways in reducing protein disulfide bonds in the Escherichia coli cytoplasm J. Biol. Chem. 272 1997 15661 15667
31. E.L. Schneider, J.G. Thomas, J.A. Bassuk, E.H. Sage, and F. Baneyx Manipulating the aggregation and oxidation of human SPARC in the cytoplasm of Escherichia coli Nat. Bio/Technol. 15 1997 581 585
32. G.S. Gendeh, L.C. Young, C.L. de Medeiros, K. Jeyaseelan, A.L. Harvey, and M.C. Chung A new potassium channel toxin from the sea anemone Heteractis magnifica: isolation, cDNA cloning, and functional expression Biochemistry 36 1997 11461 11471
33. H.W. Tedford, J.I. Fletcher, and G.F. King Functional significance of the beta hairpin in the insecticidal neurotoxin omega-atracotoxin-Hv1a J. Biol. Chem. 276 2001 26568 26576
34. B.R. Kelemen, T.A. Klink, M.A. Behlke, S.R. Eubanks, P.A. Leland, and R.T. Raines Hypersensitive substrate for ribonucleases Nucleic Acids Res. 27 1999 3696 3701
35. R.T. Raines, M. McCormick, T.R. Van Oosbree, and R.C. Mierendorf The S.Tag fusion system for protein purification Methods Enzymol. 326 2000 362 376
36. P. Jurado, D. Ritz, J. Beckwith, V. de Lorenzo, and L.A. Fernandez Production of functional single-chain Fv antibodies in the cytoplasm of Escherichia coli J. Mol. Biol. 320 2002 1 10
37. R. Levy, R. Weiss, G. Chen, B.L. Iverson, and G. Georgiou Production of correctly folded Fab antibody fragment in the cytoplasm of Escherichia coli trxB gor mutants via the coexpression of molecular chaperones Protein Express. Purif. 23 2001 338 347
38. Y. Chen, J. Song, S.F. Sui, and D.N. Wang DnaK and DnaJ facilitated the folding process and reduced inclusion body formation of magnesium transporter CorA overexpressed in Escherichia coli Protein Express. Purif. 32 2003 221 231
39. K.H. Lee, H.S. Kim, H.S. Jeong, and Y.S. Lee Chaperonin GroESL mediates the protein folding of human liver mitochondrial aldehyde dehydrogenase in Escherichia coli Biochem. Biophys. Res. Commun. 298 2002 216 224
40. H. Kadokura, F. Katzen, and J. Beckwith Protein disulfide bond formation in prokaryotes Annu. Rev. Biochem. 72 2003 111 135
41. J.U. Bowie, and R.T. Sauer Identification of C-terminal extensions that protect proteins from intracellular proteolysis J. Biol. Chem. 264 1989 7596 7602
42. D.A. Parsell, and R.T. Sauer The structural stability of a protein is an important determinant of its proteolytic susceptibility in Escherichia coli J. Biol. Chem. 264 1989 7590 7595
43. M. Ghosh, and R.F. Schleif Stabilizing C-terminal tails on AraC Proteins 42 2001 177 181