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Volumn 43, Issue 35, 2004, Pages 4612-4615

A combinatorial approach to catalytic peptide dendrimers

Author keywords

Cobalamines; Combinatorial chemistry; Dendrimers enzyme models; Peptides

Indexed keywords

AMINO ACIDS; CATALYSIS; ESTERS; HYDROLYSIS; MATHEMATICAL MODELS; PROTEINS; SYNTHESIS (CHEMICAL); VITAMINS;

EID: 5344258255     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/anie.200460177     Document Type: Article
Times cited : (86)

References (32)
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    • note
    • Single dendrimer-containing resin beads were hydrolyzed with aqueous HCl (6 M) at 110°C for 22 h, and their amino acid composition was determined quantitatively by HPLC after derivatization with phenyl isothiocyanate (PITC). Such amino acid analyses are routine for protein-composition analysis. The analysis detects as little as 5 pmol per amino acid, which is sufficient for single-resin-bead analysis, as these contain 50-200 pmol of dendrimers.
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    • note
    • False positives are most likely due to manipulation errors during bead picking. Sometimes more than one bead is transferred to the pipette, and the fluorescence staining is diluted when the beads are washed down the pipette used for picking, which does not allow staining to be rechecked afterwards.
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* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.