The dual exo/endo-type mode and the effect of ionic strength on the mode of catalysis of chitinase 60 (CHI60) from Serratia sp. TU09 and its mutants

Carbohydrate Research

Volumn 343, Issue 16, 2008, Pages 2754-2762

  Kuttiyawong, K ^a   Nakapong, S ^a   Pichyangkura, R ^a  

^a CHULALONGKORN UNIVERSITY   (Thailand)

Author keywords

Chitin; Chitinase; exo endo Type; Mutation; Tryptophan

Indexed keywords

AMORPHOUS SUBSTRATES; CATALYTIC DOMAIN; CHITINASE; COLLOIDAL CHITIN; CRYSTALLINE SUBSTRATES; DUAL MODES; EFFECT OF IONIC STRENGTH; ENZYME-SUBSTRATE INTERACTIONS; EXO/ENDO-TYPE; HYDROLYTIC ACTIVITIES; MUTANT ENZYMES; MUTATION; N-TERMINAL DOMAIN; PROCESSIVITY; SERRATIA; SUBSTRATE BINDING; SUBSTRATE TYPES; TRYPTOPHAN; TRYPTOPHAN RESIDUES; WILD-TYPE;

AMINO ACIDS; CATALYSIS; CHITIN; CHLORINE COMPOUNDS; CRYSTALLINE MATERIALS; ENZYME ACTIVITY; ENZYMES; EPITAXIAL GROWTH; FLOW INTERACTIONS; FOOD ADDITIVES; IONIC STRENGTH; POLYSACCHARIDES; SODIUM CHLORIDE;

SUBSTRATES;

CHITIN; CHITINASE; CRYSTALLIN; PHENYLALANINE; SODIUM CHLORIDE; TYROSINE;

ACETYLATION; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL STRAIN; CATALYSIS; COLLOID; COMPLEX FORMATION; DISSOCIATION; ENZYME ACTIVITY; ENZYME BINDING; ENZYME SUBSTRATE; ENZYME SUBSTRATE COMPLEX; HYDROLYSIS; IONIC STRENGTH; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SERRATIA; WILD TYPE;

BIOCATALYSIS; CHITINASE; DOSE-RESPONSE RELATIONSHIP, DRUG; HYDROLYSIS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; OSMOLAR CONCENTRATION; SERRATIA; SODIUM CHLORIDE; STRUCTURE-ACTIVITY RELATIONSHIP;

SERRATIA SP.;

EID: 53049104823     PISSN: 00086215     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.carres.2008.05.020     Document Type: Article

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