Characterization of CenC, an enzyme from Cellulomonas fimi with both endo- and exoglucanase activities

Journal of Bacteriology

Volumn 178, Issue 14, 1996, Pages 4216-4223

  Tomme, Peter ^a   Kwan, Emily ^a   Gilkes, Neil R ^a   Kilburn, Douglas G ^a   Antony, R ^a   Warren, J ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; BETA GLUCAN; CARBOXYMETHYLCELLULOSE; CELLULASE; CELLULOSE; MANNAN; MICROCRYSTALLINE CELLULOSE;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CELLULOMONAS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME PURIFICATION; HYDROLYSIS; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL;

EID: 0029896866     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.14.4216-4223.1996     Document Type: Article

References (54)

1. Atkinson, T., and M. Smith. 1984. Solid phase synthesis of oligodeoxyribonucleotides by the phosphite triester method, p. 35-81. In N. J. Gait (ed.), Oligonucleotide synthesis: a practical approach. IRL Press, Oxford.
2. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1989. Current protocols in molecular biology, vol. 1. John Wiley & Sons, Inc., New York.
3. Bailey, M. J., M. Siika-aho, A. Valkeajärvi, and M. E. Penttilä. 1993. Hydrolytic properties of two cellulases of Trichnderma reesei expressed in yeast. Biotechnol. Appl. Biochcm. 17:65-76.
4. Baker, T. A. 1984. A gene regulating the heat shock response in Escherichia coli also affects proteolysis. Proc. Natl. Acad. Sci. USA 81:6779-6783.
5. 3-labeled, reduced and chromogenic cellooligosaccharides. Biochem. J. 266:371-378.
6. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochcm. 72:248-254.
7. Bronnenmeier, K., K. P. Rücknagel, and W. L. Staudenbauer. 1991. Purification and properties of a novel type of exo-l,4-β-glucanase (Avicelase II) from the cellulolytic thermophile Clostridium. stercorarium. Eur. J. Biochem. 200:379-385.
8. Cantor, C. R., and P. R. Schimmel. 1980. Biophysical chemistry, vol. 2. W. H. Freeman & Co., San Francisco.
9. Chanzy, H. 1990. Aspects of cellulose structure, p. 3-12. In J. F. Kennedy, G. O. Phillips, and P. A. Williams (ed.). Cellulose sources and exploitation. Ellis Horwood, New York.
10. Chauvaux, S., P. Béguin, and J.-P. Aubert. 1992. Site-dircclcd mutagenesis of essential carboxylic residues in Closiridium thermocellum endoglucanase CelD. J. Biol. Chem. 26:4472-4478.
11. Christakopoulos, P., D. Kekos, B. J. Marcis, M. Claeyssens, and M. K. Bhat. 1995. Purification and characterization of a less randomly acting endo-1,4-β-D-glucanasc from the culture filtrate of Fusarium oxysporum. Arch. Biochem. Biophys. 316:428-433.
12. Claeyssens, M., and P. Tomme. 1988. Structure-function relationships of cellulolytic proteins from Trichoderma reesei, p. 1-11. In C. P. Kubicek. D. E. Eveleigh, H. Esterbauer, W. Steiner, and E. M. Kuhicek-Pranz (ed.), Trichoderma reesei cellulases: biochemistry, genetics, physiology and application. Proceedings. Tricell 1989. Royal Society of Chemistry 1990. Thomas Graham House, Cambridge.
13. Coutinho, J. B., N. R. Gilkes, R. A. J. Warren, D. G. Kilburn, and R. C. Miller, Jr. 1992. The binding of Cellulomonas fimi endoglucanasc C (CenC) to cellulose and Sephadex is mediated by the N-terminal repeats. Mol. Microbiol. 6:1243-1252.
14. Coutinho, J. B., B. Moser, D. G. Kilburn, R. A. J. Warren, and R. C. Miller, Jr. 1991. Nucleotide sequence of the endoglucanase C gene (cenC) of Cellulomonas fimi, its high-level expression in Escherichia coli, and characterization of its products. Mol. Microbiol. 5:1221-1233.
15. Divne, C., J. Ståhlberg, T. Reinikainen, L. Ruohonen, G. Petterson, J. K. C. Knowles, T. T. Teeri, and T. A. Jones. 1994. The three-dimensional crystal structure of the catalytic core of cellobiohydrolase 1 from Trichoderma reesei. Science 265:524-528.
16. Enari, T.-M., and M. L. Niku-Paavola. 1987. Enzymatic hydrolysis of cellulose: is the current theory of the mechanisms of hydrolysis valid? Crit. Rev. Biotechnol. 5:67-87.
17. Gebier, J., N. R. Gilkes, M. Claeyssens, D. B. Wilson, P. Béguin, W. W. Wakarchuk, D. G. Kilburn, R. C. Miller, Jr., R. A. J. Warren, and S. G. Withers. 1992. Stereoselective hydrolysis catalyzed by related β-1,4-glucanases and β-l,4-xylanases. J. Biol. Chem. 267:12559-12561.
18. Gilkes, N. R., E. Jervis, B. Henrissat, B. Tekant, R. C. Miller, Jr., R. A. J. Warren, and D. G. Kilburn. 1992. The adsorption of a bacterial cellulase and its two isolated domains to crystalline cellulose. J. Biol. Chem. 267:6743-6749.
19. Gilkes, N. R, M. L. Langsford, D. G. Kilburn, R. C. Miller, Jr., and R. A. J. Warren. 1984. Mode of action and substrate specificities of cellulases from cloned bacterial genes. J. Biol. Chem. 259:10455-10459.
20. Graham, R. W., J. M. Greenwood, R. A. J. Warren, D. G. Kilburn, and D. E. Trimbur. 1995. The pTugA and pTugAS vectors for high-level expression of cloned genes in Escherichia coli. Gene 158:51-54.
21. Hanahan, D. 1983. Study on the transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580.
22. Henrissal, B., and A. Bairoch. 1993. New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J. 293:781-788.
23. Joliff, G., P. Béguin, and J.-P. Aubert. 1986. Nucleotide sequence of the cellulase gene celD encoding endoglucanase D of Clostridium thermocellum. Nucleic Acids Res. 14:8605-8613.
24. Juy, M., A. G. Amit, P. M. Alzari, R. J. Poljak, M. Claeysens, P. Béguin, and J.-P. Aubert. 1992. Three-dimensional structure of a thermostable bacterial cellulase. Nature (London) 357:89-91.
25. Leatherbarrow, R. J. 1992. GruFit version 3.0. Erithacus Software Ltd., Staines. United Kingdom.
26. Lever, M. 1973. Colorimetric and fluorometric carbohydrate determination with p-hydroxybenzoic acid hydrazide. Biochem. Med. 7:274-281.
27. MacLeod, A. M.,T. Lindhorst, S. G. Withers, and R. A. J. Warren. 1994. The acid/base catalyst in the exoglucanase/xylanase (Cex) from Cellulomonas fimi is Glu 127: evidence from detailed kinetic studies of mutants. Biochemistry 33:6371-6376.
28. Matsudaira, P. 1987. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membrane. J. Biol. Chem. 262: 10035-10038.
29. Matsuura, Y., M. Kusutioki, W. Harada, and M. Kakudo. 1984. Structure and possible catalytic residues of taka-amylasc A. J. Biochem. 95:697-702.
30. Meinke, A., C. Braun, N. R. Gilkes, D. G. Kilburn, R. C. Miller, Jr., and R. A. J. Warren. 1991. Unusual sequence organization in CenB. an inverting endoglucanase from Cellulomonas fimi. J. Bacteriol. 173:308-314.
31. Meinke, A., N. R. Gilkes, E. Kwan, D. G. Kilburn, R. A. J. Warren, and R. C. Miller, Jr. 1994. Cellobiohydrolase A from the cellulolytic baclerium Cellulomonas fimi is a β-1,4-exocellobiohydrolase analogous to Trichoderma reesei CBH II. Mol. Microbiol. 12:413-422.
32. Meinke, A., and R. A. J. Warren. 1993. Molecular biology of the cellulase system of Cellulomonas fimi. Curr. Top. Mol. Genet. (Life Sci. Adv.). 1:65-73.
33. Miller, G. L., R. Blum, W. E. Glennon, and A. L. Burtnn. 1960. Measurement of carboxymethylcellulase activity. Anal. Biochem. 1:127-132.
34. Cex) domain of an exoglucanase from Cellulomonas fimi: production in Escherichia coli and characterization of the polypeptide. Biotechnol. Bioeng. 42:401-409.
35. Robyt, J. F., and D. French. 1967. Multiple attack hypothesis of alpha-amylase action: action of porcine pancreatic, human salivary and Aspergillus oryzae alpha-amylase. Arch. Biochem. Biophys. 122:8-16.
36. Rouvinen, J., T. Bergfors, T. Teeri, J. K. C. Knuwles, and T. A. Jones. 1990. Three-dimeasional structure of cellohiohydrolase 11 from Trichoderma reesei. Science 249:380-386.
37. Satnbrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, N.Y.
38. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
39. Scopes, R. K. 1974. Measurement of protein by spectrometry at 205 nm. Anal. Biochem. 59:277-282.
40. Shen, H., N. R. Gilkes, D. G. Kilburn, R. C. J. Miller, and R. A. J. Warren. 1995. Cellobiohydrolase B (CbhB), a second cellobiohydrolase from the cellulolytic bacterium Cellulomonas fimi. Biochem. J. 311:67-74.
41. Shen, H., M. Schmuck, I. Pilz, N. R. Gilkes, D. G. Kilburn, R. C. Miller, Jr., and R. A. J. Warren. 1991. Deletion of the linker connecting the catalytic and cellulose-binding domains of endoglucanase A (CenA) of Cellulomonas fimi alters its conformation and catalytic activity. J. Biol. Chem. 266:11335-11340.
42. Shen, H., P. Tomme, A. Meinke, N. R. Gilkes, D. G. Kilburn, R. A. J. Warren, and R. C. J. Miller. 1994. Stereochemical course of hydrolysis catalysed by Cellulomonas fimi CenE, a member of a new family of beta-1,4-glueanases. Biochem. Biophys. Res. Commun. 199:1223-1228.
43. Sinnot, M. L. 1990. Catalytic mechanisms of enzymic glycosyl transfer. Chem. Rev. 90:1171-1202.
44. Spezio, M., D. B. Wilson, and P. A. Karplus. 1993. Crystal structure of the catalytic domain of a thermophilic endocellulase. Biochemistry 32:9906-9916.
45. Ståhlberg, J., G. Johansson, and G. Pettersson. 1993. Trichoderma reesei has no true exo-cellulase: all intact and truncated cellulases produce new reducing end groups on cellulose. Biochim. Biophys. Acta 1157:107-113.
46. Tabor, S., and C. C. Richardson. 1987. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc. Natl. Acad. Sci. USA 84:4767-4771.
47. Teather, R. M., and P. J. Wood. 1982. Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from bovine rumen. Appl. Environ. Microbiol. 43:777-7811.
48. Tomme, P., S. Chauvaux, P. Béguin, J. Millet, J.-P. Aubert, and M. Claeyssens. 1991. Identification of a histidyl residue in the active center of endoglucanase D from Clostridium thermocellum. J. Biol. Chem. 266:10313-10318.
49. Tomme, P., and M. Claeyssens. 1989. Identification of the functional important carboxyl group in cellobiohydrolase I from Trichoderma reesei: a chemical modification study. FEBS Lett. 243:239-243.
50. Tomme, P., R. A. J. Warren, and N. R. Gilkes. 1995. Cellulose hydrolysis by bacteria and fungi. Adv. Microbial Physiol. 37:1-81.
51. Tomme, P., R. A. J. Warren, R. C. Miller. Jr., D. G. Kilburn, and N. R. Gilkes. 1995. Cellulose-binding domains: classification and properties, p. 142-161. In J. N. Saddler and M. H. Penner (ed.). Enzymatic degradation of insoluble polysaccharides, vol. 618. American Chemical Society, Washington, D. C.
52. Vrsanka, M., and P. Biely. 1992. The cellobiohydrolase I from Trichoderma reesei QM9414 action on cellooligosaccharides. Carbohydr. Res. 227:19-27.
53. Wood, P. J. 1980. Specificity of the interaction of direct dyes with polysaccharides. Carbohydr. Res. 85:271-287.
54. Yanisch-Perron, C., J. Vieira, and J. Messing. 1983. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp and pUC vectors. Gene (Amsterdam) 33:103-119.