Development of an O6-alkylguanine-DNA alkyltransferase assay based on covalent transfer of the benzyl moiety from [benzene-3H]O6-benzylguanine to the protein

Analytical Biochemistry

Volumn 383, Issue 1, 2008, Pages 44-51

  Ishiguro, Kimiko ^a   Shyam, Krishnamurthy ^a   Penketh, Philip G ^a   Sartorelli, Alan C ^a  

^a YALE CANCER CENTER   (United States)

Author keywords

AGT positive and negative cells; Carmustine; Cloretazine; Drug binding to melanin; Temozolomide

Indexed keywords

BENZENE; CELL CULTURE; ELECTROPHORESIS; MELANIN; MOLECULES; RADIOACTIVITY; TUMORS;

CARMUSTINE; CLINICAL PRACTICES; CLORETAZINE; DRUG BINDING; HUMAN CELL LINES; SECOND ORDER KINETICS; TEMOZOLOMIDE; WESTERN-BLOT ANALYSIS;

CELLS;

6 O ALKYLGUANINE DNA ALKYLTRANSFERASE; 6 O BENZYLGUANINE; BENZENE DERIVATIVE; MELANIN; METHANOL;

ANIMAL CELL; ARTICLE; CELL FREE SYSTEM; ENZYME ACTIVITY; ENZYME ASSAY; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; RADIOACTIVITY; RAT; TUMOR RESISTANCE; WESTERN BLOTTING;

MURINAE; RODENTIA;

BLOTTING, WESTERN; CELL LINE; ENZYME ASSAYS; GUANINE; HUMANS; O(6)-METHYLGUANINE-DNA METHYLTRANSFERASE; PROTEINS; REPRODUCIBILITY OF RESULTS;

EID: 53049090483     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.08.009     Document Type: Article

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