Functional assembly of a microbial consortium with autofluorescent and mineralizing activity for the biodegradation of organophosphates

Journal of Agricultural and Food Chemistry

Volumn 56, Issue 17, 2008, Pages 7897-7902

  Zhang, Heng ^a,b   Yang, Chao ^a,b   Li, Chaokun ^a,b   Li, Lin ^c   Zhao, Qiao ^d   Qiao, Chuanling ^a  

^a INSTITUTE OF ZOOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c RESEARCH CENTER FOR ECO ENVIRONMENTAL SCIENCES   (China)

^d OHIO STATE UNIVERSITY   (United States)

Author keywords

Coexpression; Green fluorescent protein; Methyl parathion hydrolase; Microbial consortium; Organophosphate

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; ORGANOPHOSPHORUS COMPOUND; PESTICIDE; PHOSPHATASE; RECOMBINANT PROTEIN;

ARTICLE; BIOREMEDIATION; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC ENGINEERING; GENETICS; METABOLISM; SPECTROFLUOROMETRY;

BIODEGRADATION, ENVIRONMENTAL; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC ENGINEERING; GREEN FLUORESCENT PROTEINS; ORGANOPHOSPHORUS COMPOUNDS; PESTICIDES; PHOSPHORIC MONOESTER HYDROLASES; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; OCHROBACTRUM; OCHROBACTRUM SP.;

EID: 52649170330     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf801684g     Document Type: Article

References (40)

1. Zhang, R.; Cui, Z. L.; Jiang, J.; He, J.; Gu, X.; Li, S. P. Diversity of organophosphorus pesticide-degrading bacteria in a polluted soil and conservation of their organophosphorus hydrolase genes. Can. J. Microbiol. 2005, 51, 337-343.
2. Mulbry, W. W.; Karns, J. S. Parathion hydrolase specified by the Flavobacterium opd gene: relationship between the gene and protein. J. Bacteriol. 1989, 171, 6740-6746.
3. Serdar, C. M.; Murdock, D. C.; Rohde, M. F. Parathion hydrolase gene from Pseudomonas diminuta MG: subcloning, complete nucleotide sequence, and expression of the mature portion of the enzyme in Escherichia coli. Biotechnology 1989, 7, 1151-1155.
4. Horne, I.; Sutherland, T. D.; Harcourt, R. L.; Russell, R. J.; Oakeshott, J. G. Identification of an opd (organophosphate degradation) gene in an Agrobacterium isolate. Appl. Environ. Microbiol. 2002, 68, 3371-3376.
5. Cui, Z. L.; L,i, S. P.; Fu, G. P. Isolation of methyl parathion-degrading strain M6 and cloning of the methyl parathion hydrolase gene. Appl. Environ. Microbiol. 2001, 67, 4922-4925.
6. Fu, G. P.; Cui, Z. L.; Huang, T.; L,i, S. P. Expression, purification, and characterization of a novel methyl parathion hydrolase. Protein Expression Purif. 2004, 36, 170-176.
7. Yang, C.; Cai, N.; Dong, M.; Jiang, H.; Li, J.; Qiao, C. L.; Mulchandani, A.; Chen, W. Surface display of MPH on Pseudomonas putida JS444 using ice nucleation protein and its application in detoxification of organophosphates. Biotechnol. Bioeng. 2008, 99, 30-37.
8. Cormack, B. P.; Valdivia, R. H.; Falkow, S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 1996, 173, 33-38.
9. Nolte, C.; Matyash, M.; Pivneva, T.; Schipke, C. G; Ohlemeyer, C.; Hanisch, U. K; Kirchhoff, F.; Kettenmann, H. GFAP promoter controlled EGFP-expressing transgenic mice: a tool to visualize astrocytes and astrogliosis in living brain tissue. Glia 2001, 33, 72-86.
10. Halfhill, M. D; Sutherland, J. P; Moon, H. S; Poppy, G. M.; Warwick, S. I.; Weissinger, A. K; Rufty, T. W.; Raymer, P. L.; Stewart, C N, Jr. Growth, productivity, and competitiveness of introgressed weedy Brassica rapa hybrids selected for the presence of Bt cry1Ac and gfp transgenes. Mol. Ecol. 2005, 14, 3177-3189.
11. Jansson, J. K. Marker and reporter genes: Illuminating tools for environmental microbiologists. Curr. Opin. Microbiol. 2003, 6, 310-316.
12. Tresse, O.; Errampalli, D.; Kostrzynska, M.; Leung, K. T.; Lee, H.; Trevors, J. T; Elsas, J. D. Green fluorescent protein as a visual marker in a p-nitrophenol degrading Moraxella sp. FEMS Microbiol. Lett. 1998, 164, 187-193.
13. Errampalli, D.; Leung, K.; Cassidy, M. B.; Kostrzynska, M.; Blears, M.; Lee, H.; Trevors, J. T. Applications of the green fluorescent protein as a molecular marker in environmental microorganisms. J. Microbiol. Methods 1999, 35, 187-199.
14. Li, C.; Zhu, Y.; Benz, I.; Schmidt, M. A.; Chen, W.; Mulchandani, A.; Qiao, C. L. Presentation of functional organophosphorus hydrolase fusions on the surface of Escherichia coli by the AIDA-I autotransporter pathway. Biotechnol. Bioeng. 2008, 99, 485-490.
15. March, J. C.; Rao, G.; Bentley, W. E. Biotechnological applications of green fluorescent protein. Appl. Microbiol. Biotechnol. 2003, 62, 303-315.
16. Gory, L.; Montel, M. C; Zagorec, M. Use of green fluorescent protein to monitor Lactobacillus sakei in fermented meat products. FEMS Microbiol. Lett. 2001, 194, 127-133.
17. Chalova, V.; Woodward, C. L.; Ricke, S. C. Application of an Escherichia coli green fluorescent protein-based lysine biosensor under nonsterile conditions and autofluorescence background. Lett. Appl. Microbiol. 2006, 42, 265-270.
18. Lei, Y.; Mulchandani, A.; Chen, W. Improved degradation of organophosphorus nerve agents and p-nitrophenol by Pseudomonas putida JS444 with surface-expressed organophosphorus hydrolase. Biotechnol. Prog. 2005, 21, 678-681.
19. Richins, R. D; Kaneva, I.; Mulchandani, A.; Chen, W. Biodegradation of organophosphorus pesticides by surface-expressed organophosphorus hydrolase. Nat. Biotechnol. 1997, 15, 984-987.
20. Shimazu, M.; Nuyen, A.; Mulchandani, A.; Chen, W. Cell surface display of organophosphorus hydrolase in Pseudomonas putida using ice nucleation protein anchor. Biotechnol. Prog. 2003, 19, 1612-1614.
21. Arfmann, H. A.; Timmis, K. N.; Wittich, R. M. Mineralization of 4-chlorodibenzofuran by a consortium consisting of Sphingomonas sp. strain RW1 and Burkholderia sp. strain JWS. Appl. Environ. Microbiol. 1997, 63, 3458-3462.
22. Casellas, M.; Grifoll, M.; Sabata, J.; Solanas, A. M. Isolation and characterization of a 9-fluorenone-degrading bacterial strain and its role in synergistic degradation of fluorine by a consortium. Can. J. Microbiol. 1998, 44, 734-742.
23. Gilbert, E. S.; Walker, A. W.; Keasling, J. D. A constructed microbial consortium for biodegradation of the organophosphorus insecticide parathion. Appl. Microbiol. Biotechnol. 2003, 61, 77-81.
24. Xu, G.; Li, Y.; Zheng, W.; Peng, X.; Li, W.; Yan, Y. Mineralization of chlorpyrifos by co-culture of Serratia and Trichosporon spp. Biotechnol. Lett. 2007, 29, 1469-1473.
25. Yang, C.; Liu, N.; Guo, X. M.; Qiao, C. L. Cloning of mpd gene from a chlorpyrifos-degrading bacterium and use of this strain in bioremediation of contaminated soil. FEMS Microbiol. Lett. 2006, 265, 118-125.
26. Sambrook, J.; Russel, D. W. Molecular Cloning: A Laboratory Manual, 3rd ed.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, 2001.
27. Bradford, M. M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 1976, 72, 248-254.
28. Qiu, X.; Bai, W.; Zhong, Q.; Li, M.; He, F.; Li, B. Isolation and characterization of a bacterial strain of the genus Ochrobactrum with methyl parathion mineralizing activity. J. Appl. Microbiol. 2006, 101, 986-994.
29. Spain, J. C.; Gibson, D. T. Pathway for biodegradation of para-nitrophenol in a Moraxella sp. Appl. Environ. Microbiol. 1991, 57, 812-819.
30. Jain, R. K.; Dreisbach, J. H.; Spain, J. C. Biodegradation of p-nitrophenol via 1,2,4-benzenetriol by an Arthrobacter sp. Appl. Environ. Microbiol. 1994, 60, 3030-3032.
31. Scheich, C.; Kümmel, D; Soumailakakis, D; Heinemann, U; Büssow, B. Vectors for co-expression of an unrestricted number of proteins. Nucleic Acids Res. 2007, 35, e43.
32. Li, C.; Schwabe, J. W.; Banayo, E.; Evans, R. M. Coexpression of nuclear receptor partners increases their solubility and biological activities. Proc. Natl. Acad. Sci. U.S.A. 1997, 94, 2278-2283.
33. Lan, W. S.; Gu, J. D; Shen, B. C; Jiang, H.; Qiao, C. L.; Mulchandani, A.; Chen, W. Coexpression of two detoxifying pesticide-degrading enzymes in a genetically engineered bacterium. In. Biodeterior. Biodegrad. 2006, 58, 70-76.
34. Wladyka, B.; Puzia, K.; Dubin, A. Efficient co-expression of a recombinant staphopain A and its inhibitor staphostatin A in Escherichia coli. Biochem. J. 2005, 385, 181-187.
35. Singh, B. K.; Walker, A. Microbial degradation of organophosphorus compounds. FEMS Microbiol. Rev. 2006, 30, 428-471.
36. Strong, L. C.; McTavish, H.; Sadowsky, M. J.; Wackett, L. P. Field-scale remediation of atrazine-contaminated soil using recombinant Escherichia coli expressing atrazine chlorohydrolase. Environ. Microbiol. 2000, 2, 91-98.
37. Liu, Y.; Li, J. Role of Pseudomonas aeruginosa Biofilm in the initial adhesion, growth and detachment of Escherichia coli in porous media. Environ. Sci. Technol. 2008, 42, 443-449.
38. Cha, H. J; Wu, C. F.; Rao, G.; Valdes, J. J.; Bentley, W. E. Observations of green fluorescent protein as a fusion partner in genetically engineered Escherichia coli: monitoring protein expression and solubility. Biotechnol. Bioeng. 2000, 67, 565-574.
39. Wu, C. F.; Cha, H. J; Rao, G.; Valdes, J. J.; Bentley, W. E. A green fluorescent protein fusion strategy for monitoring the expression, cellular location, and separation of biologically active organophosphorus hydrolase. Appl. Microbiol. Biotechnol. 2000, 54, 78-83.
40. Wu, C. F.; Cha, H. J.; Valdes, J. J.; Bentley, W. E. GFP-visualized immobilized enzymes: Degradation of paraoxon via organophosphorus hydrolase in a packed column. Biotechnol. Bioeng. 2002, 77, 212-218.