Single-step purification of recombinant green fluorescent protein on expanded beds of immobilized metal affinity chromatography media

Biochemical Engineering Journal

Volumn 42, Issue 3, 2008, Pages 301-307

  Dalal, Sohel ^a   Raghava, Smita ^a   Gupta, M N ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

Author keywords

Adsorption; Bioseparation; Fluidization; Green fluorescent protein; Immobilized metal affinity chromatography; Purification

Indexed keywords

AFFINITY CHROMATOGRAPHY; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; ESCHERICHIA COLI; FLUORESCENCE; ION CHROMATOGRAPHY; LIGHT EMISSION; LUMINESCENCE; METAL IONS; METAL REFINING; METALS; NICKEL; PROTEINS;

ADSORPTION; BIOSEPARATION; EXPANDED BEDS; FLUIDIZATION; GREEN FLUORESCENT PROTEIN; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; IMMOBILIZED METAL ION AFFINITY CHROMATOGRAPHY; SINGLE-STEP PURIFICATION;

PURIFICATION;

ALGINIC ACID; GREEN FLUORESCENT PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; CHROMATOGRAPHY; ESCHERICHIA COLI; EXPANDED BED ADSORPTION; GENE OVEREXPRESSION; IMMUNOAFFINITY CHROMATOGRAPHY; LANGMUIR BLODGETT FILM; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN PURIFICATION;

ESCHERICHIA COLI;

EID: 52049116092     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2008.07.010     Document Type: Article

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