Expanded bed recovery of D-2-chloropropionic acid dehalogenase using TiO2-densified cellulose anion exchanger

Chinese Journal of Chemical Engineering

Volumn 14, Issue 4, 2006, Pages 505-510

  Lei, Yinlin ^a   Jin, Zhihua ^a   Tong, Weixing ^a   Yao, Shanjing ^a   Zhu, Ziqiang ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

2 chloropropionic acid; Cellulose; Dehalogenase; Expanded bed adsorption; Ion exchange

Indexed keywords

ADSORPTION; CELLULOSE; CHROMATOGRAPHY; COMPOSITE MATERIALS; DENSIFICATION; ION EXCHANGE; ION EXCHANGERS; PROTEINS; PURIFICATION; TITANIUM DIOXIDE;

CELLULOSE ANION EXCHANGER; D-2-CHLOROPROPIONIC ACID; DEHALOGENASE; EXPANDED BED ADSORPTION; PURIFICATION FACTOR;

ENZYMES;

EID: 33749833452     PISSN: 10049541     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1004-9541(06)60105-5     Document Type: Article

References (16)

1. Ordaz, E., Garrido-Pertierra, A., Gallego, M., Puyet, A., Covalent and metal-chelate immobilization of a modified 2-haloacid dehalogenase for the enzymatic resolution of optically active chloropropionic acid, Biotechnol. Progr., 16, 287-291(2000).
2. Anspach, F. B., Curbelo, D., Hartmann, R., Garke, G., Deckwer, W. D., Expanded bed chromatography in primary protein purification, J. Chromatogr. A, 865, 129-144(1999).
3. Olander, M. A., Jakobsen, U. L., Hansen, M. B., Lihme, A., Fractionation of high-value whey proteins, Scandinavian Dairy Information, 2, 22-25 (2001).
4. Zhou, X., Shi, Q. H., Bai, S., Sun, Y., Dense pellicular agarose-glass beads for expanded bed application: Flow hydrodynamics and solid phase classifications, Chin. J. Chem. Eng., 12, 310-314(2004).
5. Gemeiner, P., Polakovic, M., Mislovicova, D., Stefuca, V., Cellulose as a (bio)affinity carrier: Properties, design and applications, J. Chromatogr. B, 715, 245-271(1998).
6. Lei, Y. L., Lin, D. Q., Yao, S. J., Liu, Z. Z., Zhu, Z. Q, Physical and hydrodynamic properties of spherical cellulose-titanium dioxide composite matrix for expanded bed adsorption, Chin. J. Chem. Eng., 11, 141-145(2003).
7. Lei, Y. L., Lin, D. Q., Yao, S. J., Zhu, Z. Q., Preparation and characterization of titanium oxide-densified cellulose beads for expanded bed adsorption, J. Appl. Polym. Sci., 90, 2848-2854(2003).
8. 2-desified cellulose beads for expanded bed adsorption, Reac. Func. Polym., 62, 167-177(2005).
9. Bradford, M., A rapid and sensitive method for the quantition of microgram quantities of protein utilizing the principle of dye-binding, Anal. Biochem., 72, 248-254(1976).
10. Bergman, J. G., Sanik, J., Determination of trace amounts of chlorine in naphtha, Anal. Chem., 29, 241-243(1957).
11. Neilands, J. B., Lactic dehydrogenase of heart muscle, Methods Enzymol., 1, 449-454(1955).
12. Fernandez-Lahore, H. M., Geilenkirchen, S., Boldt, K., Nagel, A., Kula, M. R., Thommes, J., The influence of cell adsorbent interactions on protein adsorption in expanded beds, J. Chromatogr. A, 873, 195-208(2000).
13. Lin, D. Q., Fernandez-Lahore, H. M., Kula, M. R., Thommes, J., Minimising biomass/adsorbent interactions in expanded bed adsorption processes: A methodological design approach, Bioseparation, 10, 7-19(2001).
14. Keuning, S., Janssen, D. B., Witholt, B., Purification and characterization of hydrolytic haloalkane dehalogenase from Xanthobacter autotrophicus GJ10, J. Bacteriol., 163, 635-639(1985).
15. Motosugi, K., Esaki N., Soda K., Purification and properties of a new enzyme, DL-2-haloacid dehalogenase, from Pseudomonas sp. J. Bacteriol., 150, 522-527(1982).
16. Van den Wijinaard, A. J., Reuvekamp, P. T. W., Janssen, D. B., Purification and characterization of haloalcohol dehalogenase from Arthrobacter sp. Strain AD2, J. Bacteriol., 173, 124-129(1991).