CHO cells adapted to hypothermic growth produce high yields of recombinant β-interferon

Biotechnology Progress

Volumn 24, Issue 4, 2008, Pages 898-906

  Sunley, Kevin ^a   Tharmalingam, Tharmala ^a   Butler, Michael ^a  

^a UNIVERSITY OF MANITOBA   (Canada)

Author keywords

Adaptation; CHO; Controlled proliferation; Hypothermic; Interferon ; Microcarrier; Shear sensitivity; Temperature shift

Indexed keywords

ADAPTATION; CHO; CHO CELLS; CONTROLLED PROLIFERATION; HYPOTHERMIC; HYPOTHERMIC CONDITIONS; HYPOTHERMIC GROWTH; MICROCARRIER; SHEAR SENSITIVITY; TEMPERATURE SHIFT;

BETA INTERFERON; RECOMBINANT PROTEIN;

ANIMAL; BIOTECHNOLOGY; CELL; CELL PROLIFERATION; CHO CELL; COLD; CONFERENCE PAPER; CRICETULUS; CULTURE TECHNIQUE; CYTOLOGY; GENE EXPRESSION; GENETICS; HAMSTER; HUMAN; METABOLISM;

ANIMALS; BIOTECHNOLOGY; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CELLS; CHO CELLS; COLD TEMPERATURE; CRICETINAE; CRICETULUS; GENE EXPRESSION; HUMANS; INTERFERON-BETA; RECOMBINANT PROTEINS;

CRICETULUS GRISEUS; MAMMALIA;

EID: 51849166382     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1002/btpr.9     Document Type: Conference Paper

References (24)

1. Fox SR, Patel UA, Yap MG, Wang DI. Maximizing interferon-γ production by Chinese hamster ovary cells through temperature shift optimization; experimental and modeling. Biotechnol Bioeng. 2004;85:177-184.
2. Chen ZL, Wu BC, Liu H, Liu XM, Huang PT. Temperature shift as a process optimization step for the production of pro-urokinase by a recombinant Chinese hamster ovary cell line in high-density perfusion culture. J Biosci Bioeng. 2004;97:239-243.
3. Moore A, Mercer J, Dutina G, Donahue CJ, Bauer KD, Mather JP, Etcheverry T, Ryll T. Effects of temperature shift on cell cycle, apoptosis and nucleotide pools in CHO cell batch cultures. Cytotechnology. 1997;23:47-54.
4. Fujita J. Cold shock response in mammalian cells. J Mol Microbiol Biotechnol. 1999;1:243-255.
5. Fox SR, Tan HK, Tan MC, Wong SC, Yap MG, Wang DI. A detailed understanding of the enhanced hypothermic productivity of interferon-γ by Chinese-hamster ovary cells. Biotechnol Appl Biochem. 2005;41(Part 3):255-264.
6. Sakurai T, Itoh K, Liu Y, Higashitsuji H, Sumitomo Y, Sakamaki K, Fujita J. Low temperature protects mammalian cells from apoptosis initiated by various stimuli in vitro. Exp Cell Res. 2005;309:264-272.
7. Rodriguez J, Spearman M, Huzel N, Butler M. Enhanced production of monomeric interferon-beta by CHO cells through the control of culture conditions. Biotechnol Prog. 2005;21:22-30.
8. Chuppa S, Tsai YS, Yoon S, Shackleford S, Wales C, Bhat R, Tsay G, Matanguihan C, Konstantinov K, Naveh D. Fermentor temperature as a tool for control of high-density perfusion cultures of mammalian cells. Biotechnol Bioeng. 1997;55:328-338.
9. Shi M, Xie Z, Yu M, Shen B, Guo N. Controlled growth of Chinese hamster ovary cells and high expression of antibody-IL-2 fusion proteins by temperature manipulation. Biotechnol Lett. 2005;27(23-24):1879-1884.
10. Yoon SK, Hwang SO, Lee GM. Enhancing effect of low culture temperature on specific antibody productivity of recombinant Chinese hamster ovary cells: clonal variation. Biotechnol Prog. 2004;20:1683-1638.
11. Al-Fageeh MB, Marchant RJ, Carden MJ, Smales CM. The cold-shock response in cultured mammalian cells: harnessing the response for the improvement of recombinant protein production. Biotechnol Bioeng. 2006;93:829-835.
12. Mazur X; Fussenegger M; Renner WA. Bailey JE. Higher productivity of growth-arrested Chinese hamster ovary cells expressing the cyclin-dependent kinase inhibitor p27. Biotechnol Prog. 1998;14:705-713.
13. Palermo DP, DeGraaf ME, Marotti KR, Rehberg E, Post LE. Production of analytical quantities of recombinant proteins in Chinese hamster ovary cells using sodium butyrate to elevate gene expression. J Biotechnol. 1991;19:35-47.
14. Altamirano C, Cairo JJ, Godia F. Decoupling cell growth and product formation in Chinese hamster ovary cells through metabolic control. Biotechnol Bioeng. 2001;76:351-360.
15. Fussenegger M, Schlatter S, Daetwyler D, Mazur X, Bailey JE. Controlled proliferation by multigene metabolic engineering enhances the productivity of Chinese hamster ovary cells. Nat Biotechnol. 1998;16:468-472.
16. Kumar N, Gammell P, Clynes M. Proliferation control strategies to improve productivity and survival during CHO based production culture. Cytotechnology. 2007;53:33-46.
17. Fox SR, Yap MX, Yap MG, Wang DI. Active hypothermic growth: a novel means for increasing total interferon-gamma production by Chinese-hamster ovary cells. Biotechnol Appl Biochem. 2005;41(Part 3):265-272.
18. Yoon SK, Hong JK, Choo SH, Song JY, Park HW, Lee GM. Adaptation of Chinese hamster ovary cells to low culture temperature: cell growth and recombinant protein production. J Biotechnol. 2006;122:463-472.
19. Spearman M, Rodriguez J, Huzel N, Butler M. Production and glycosylatton of recombinant β-interferon in suspension and cytopore microcarrier cultures of CHO cells. Biotechnol Prog. 2005;21:31-39.
20. Erenpreisa J, Cragg MS. Mitotic death: a mechanism of survival? A review. Cancer Cell Int. 2001;1-7.
21. Nishiyama H, Itoh K, Kaneko Y, Kishishita M, Yoshida O, Fujita J. A glycine-rich RNA-binding protein mediating cold-inducible suppression of mammalian cell growth. J Cell Biol. 1997;137:899-908.
22. Hong JK, Kim YG, Yoon SK, Lee GM. Down-regulation of cold-inducible RNA-binding protein does not improve hypothermic growth of Chinese hamster ovary cells producing erythropoietin. Metab Eng. 2007;9:208-216.
23. GE Healthcare. "Microcarrier Cell Culture: Principles and Methods." 18-1140-62 Elanders Tofters AB: Mölnlycke, SE, 2005; pp 67-68.
24. Basu A, Yang K, Wang M, Liu S, Chintala R, Palm T, Zhao H, Peng P, Wu D, Zhang Z, Hua J, Hsieh MC, Zhou J, Petti G, Li X, Janjua A, Mendez M, Liu J, Longley C, Mehlig M, Borowski V, Viswanathan M, Filpula D. Structure-function engineering of interferon-beta-1b for improving stability, solubility, potency, immunogenicity, and pharmacokinetic properties by site-selective mono-PEGylation. Bioconjug Chem. 2006;17:618-630.