Clozapine-induced hepatotoxicity in rat hepatocytes by gel entrapment and monolayer culture

Toxicology in Vitro

Volumn 22, Issue 7, 2008, Pages 1754-1760

  Lu, Yanhua ^a   Meng, Qin ^a   Zhang, Guoliang ^b   Bei, Xiaoshu ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b ZHEJIANG UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

Clozapine; Gel entrapment; Hepatotoxicity; Rat hepatocytes

Indexed keywords

CLOZAPINE; CYTOCHROME P450; CYTOCHROME P450 INHIBITOR; DIETHYLDITHIOCARBAMIC ACID; FLUVOXAMINE; GLYCYRRHIZIC ACID; KETOCONAZOLE; OXIDIZING AGENT; REACTIVE OXYGEN METABOLITE;

ANIMAL CELL; ANIMAL MODEL; ARTICLE; CELL CULTURE; CONTROLLED STUDY; GEL; LIVER CELL; LIVER TOXICITY; MALE; MONOLAYER CULTURE; NONHUMAN; PRESSURE; PROTEIN EXPRESSION; RAT;

ANIMALS; ANTIPSYCHOTIC AGENTS; CELLS, CULTURED; CLOZAPINE; CYTOCHROME P-450 CYP2E1; CYTOCHROME P-450 CYP3A; GLYCYRRHIZIC ACID; HEPATOCYTES; MALE; OXIDATIVE STRESS; RATS; RATS, SPRAGUE-DAWLEY; REACTIVE OXYGEN SPECIES; TOXICITY TESTS;

RATTUS;

EID: 51749100410     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tiv.2008.08.002     Document Type: Article

References (30)

1. Berthiaume F., Moghe P.V., Toner M., and Yarmush M.L. Effect of extracellular matrix topology on cell structure, function, and physiological responsiveness: hepatocytes cultured in a sandwich configuration. Journal of the Federation of American Societies for Experimental Biology 10 (1996) 1471-1484
2. Boelsterli U.A., Bouis P., and Donatsch P. Relative cytotoxicity of psychotropic drugs in cultured rat hepatocytes. Cell Biology and Toxicology 3 (1987) 231-250
3. Bondolfi G., Morel F., Crettol S., Rachid F., Baumann P., and Eap C.B. Increased clozapine plasma concentrations and side effects induced by smoking cessation in 2 CYP 1A2 genotyped patients. Therapeutic Drug Monitoring 27 (2005) 539-543
4. Brandon E.F., Raap C.D., Meijerman I., Beijnen J.H., and Schellens J.H. An update on in vitro test methods in human hepatic drug biotransformation research: pros and cons. Toxicology and Applied Pharmacology 189 (2003) 233-246
5. Brøsen K., Skjelbo E., and Rasmussen B.B. Fluvoxamine is a potent inhibitor of cytochrome P450 1A2. Biochemical Pharmacology 45 (1993) 1211-1214
6. Buur-Rasmussen B., and Brosen K. Cytochrome P450 and therapeutic drug monitoring with respect to clozapine. European Neuropsychopharmacology 9 (1999) 453-459
7. Dain J.G., Nicoletti J., and Ballard F. Biotransformation of clozapine in humans. Drug Metabolism and Disposition 25 (1997) 603-609
8. Diaz F.J., de Leon J., Josiassen R.C., Cooper T.B., and Simpson G.M. Plasma clozapine concentration coefficients of variation in a long-term study. Schizophrenia Research 72 (2005) 131-135
9. Erdogan A., Kocabasoglu N., Yalug I., Ozbay G., and Senturk H. Management of marked liver enzyme increase during clozapine treatment: a case report and review of the literature. The International Journal of Psychiatry in Medicine 34 (2004) 83-89
10. Fischer V., Haar J.A., Greiner L., Lloyd R.V., and Mason R.P. Possible role of free radical formation in clozapine (Clozaril)-induced agranulocytosis. Molecular Pharmacology 4 (1991) 846-853
11. Fischer V., Vogels B., Maure G., and Tynes R.E. The antipsychotic clozapine is metabolised by the polymorphic human microsomal and recombinant cytochrome P450 2D6. The Journal of Pharmacology and Experimental Therapeutics 280 (1992) 1355-1360
12. Hummer M., Kurz M., Kurzthaler I., Oberbauer H., Miller C., and Fleischhacker W.W. Hepatotoxicity of clozapine. Journal of Clinical Psychopharmacology 17 (1997) 314-317
13. Li A.P., Lu C., Brent J.A., Pham C., Fackett A., Ruegg C.E., and Silber P.M. Cryopreserved human hepatocytes: characterization of drug-metabolizing enzyme activities and applications in higher throughput screening assays for hepatotoxicity, metabolic stability, and drug-drug interaction potential. Chemico-Biological Interactions 121 (1999) 17-35
14. Linnet K., and Olesen O.V. Metabolism of clozapine by cDNA-expressed human cytochrome P450 enzymes. Drug Metabolism and Disposition 25 (1997) 1379-1382
15. Luo D., McColl P., and Walmsley R. Acute onset of ascites with clozapine-induced hepatitis. Journal of Internal Medicine 37 (2007) 204-205
16. Macfarlane B., Davies S., Mannan K., Sarsam R., Pariente D., and Dooley J. Fatal acute fulminant liver failure due to clozapine: a case report and review of clozapine-induced hepatotoxicity. Gastroenterology 112 (1997) 1707-1709
17. Masi G., Mucci M., and Pari C. Children with schizophrenia: clinical picture and pharmacological treatment. Central Nervous System Drug 20 (2006) 841-866
18. Masubuchi N., Makino C., and Murayama N. Prediction of in vivo potential for metabolic activation of drugs into chemically reactive intermediate: correlation of in vitro and in vivo generation of reactive intermediates and in vitro glutathione conjugate formation in rats and humans. Chemical Research in Toxicology 20 (2007) 455-464
19. Meng Q. Hypothermic preservation of hepatocytes. Biotechnology Progress 19 (2003) 1118-1127
20. Meng Q., Ru J., Zhang G., Shen C., Schmitmeier S., and Bader A. Re-evaluation of tacrine hepatotoxicity using gel entrapped hepatocytes. Toxicology Letter 168 (2007) 140-147
21. Nieusma J.L., Claffey D.J., Ruth J.A., and Ross D. Stereochemical aspects of the conjugation of epoxide metabolites of butadiene with glutathione in rat liver cytosol and freshly isolated rat hepatocytes. Toxicological Sciences 43 (1998) 102-109
22. Pereira A., and Dean B. Clozapine bioactivation induces dose-dependent, drug-specific toxicity of human bone marrow stromal cells: a potential in vitro system for the study of agranulocytosis. Biochemical Pharmacology 72 (2006) 783-793
23. Pirmohamed M., Williams D., Madden S., Templeton E., and Park B.K. Metabolism and bioactivation of clozapine by human liver in vitro. The Journal of Pharmacology and Experimental Therapeutics 272 (1995) 984-990
24. Prior T.I., and Baker G.B. Interactions between the cytochrome P450 system and the second-generation antipsychotics. The Journal of Psychiatry and Neuroscience 28 (2003) 99-112
25. Shen C., Zhang H., Zhang G., and Meng Q. Isoniazid-induced hepatotoxicity in rat hepatocytes of gel entrapment culture. Toxicology Letter 167 (2006) 66-74
26. Wang H.Z., Chang C.H., Lin C.P., and Tsai M.C. Using MTT viability assay to test the cytotoxicity of antibiotics and steroid to cultured porcine corneal endothelial cells. Journal of Ocular Pharmacology and Therapeutics 12 (1996) 35-43
27. Williams D.P., O'Donnell C.J., Maggs J.L., Leeder J.S., Uetrecht J., Pirmohamed M., and Park B.K. Bioactivation of clozapine by murine cardiac tissue in vivo and in vitro. Chemical Research in Toxicology 16 (2003) 1359-1364
28. Williams D.P., Pirmohamed M., Naisbitt D.J., Maggs J.L., and Park B.K. Neutrophil cytotoxicity of the chemically reactive metabolite(s) of clozapine: possible role in agranulocytosis. The Journal of Pharmacology and Experimental Therapeutics 283 (1997) 1375-1382
29. Wu D.Q., Zhang G.L., Shen C., Zhao Q., Li H., and Meng Q. Evaluation of diffusion in gel entrapment cell culture within hollow fibers. World Journal of Gastroenterology 11 (2005) 1599-1604
30. Zhou S., Chan E., Duan W., Huang M., and Chen Y.Z. Drug bioactivation, covalent binding to target proteins and toxicity relevance. Drug Metabolism Reviews 37 (2005) 41-213