Prolonged irradiation of enhanced cyan fluorescent protein or Cerulean can invalidate Förster resonance energy transfer measurements

Journal of Biomedical Optics

Volumn 13, Issue 3, 2008, Pages

  Hoffmann, Birgit ^a   Zimmer, Thomas ^a   Klöcker, Nikolaj ^b   Kelbauskas, Laimonas ^a,d   König, Karsten ^c   Benndorf, Klaus ^a   Biskup, Christoph ^a  

^a JENA UNIVERSITY HOSPITAL   (Germany)

^b UNIVERSITY OF FREIBURG   (Germany)

^c SAARLAND UNIVERSITY   (Germany)

^d ARIZONA STATE UNIVERSITY   (United States)

Author keywords

Cerulean; enhanced cyan fluorescent protein; fluorescence lifetime imaging; fluorescence resonance energy transfer; F rster resonance energy transfer; photobleaching; photoconversion; streak camera; time resolved spectroscopy

Indexed keywords

CERULEAN; ENHANCED CYAN FLUORESCENT PROTEIN; FLUORESCENCE LIFETIME IMAGING; FLUORESCENCE RESONANCE ENERGY TRANSFER; PHOTOCONVERSION; TIME-RESOLVED SPECTROSCOPY;

CAMERAS; CLONING; ENERGY TRANSFER; IRRADIATION; LASER SPECTROSCOPY; PHOTOBLEACHING; PROTEINS; RESONANCE;

FLUORESCENCE;

CYAN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN;

ARTICLE; ARTIFACT; CELL LINE; FLUORESCENCE MICROSCOPY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; KIDNEY; LIGHT; METABOLIC CLEARANCE RATE; METABOLISM; METHODOLOGY; RADIATION DOSE; RADIATION EXPOSURE; SENSITIVITY AND SPECIFICITY;

ARTIFACTS; CELL LINE; FLUORESCENCE RESONANCE ENERGY TRANSFER; GREEN FLUORESCENT PROTEINS; HUMANS; KIDNEY; LIGHT; METABOLIC CLEARANCE RATE; MICROSCOPY, FLUORESCENCE; RADIATION DOSAGE; SENSITIVITY AND SPECIFICITY;

EID: 51649100257     PISSN: 10833668     EISSN: 15602281     Source Type: Journal    
DOI: 10.1117/1.2937829     Document Type: Article

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