Role of N-terminal amino acids in the potency of anthrax lethal factor

PLoS ONE

Volumn 3, Issue 9, 2008, Pages

  Gupta, Pradeep K ^a   Moayeri, Mahtab ^a   Crown, Deborah ^a   Fattah, Rasem J ^a   Leppla, Stephen H ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; ANTHRAX LETHAL FACTOR; ANTHRAX TOXIN; UNCLASSIFIED DRUG; BACTERIAL ANTIGEN; BACTERIAL TOXIN; BLOOD CLOTTING FACTOR 10A; ZINC;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANIMAL CELL CULTURE; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIAL VIRULENCE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; FEMALE; MOLECULAR CLONING; MOUSE; MUTATIONAL ANALYSIS; NONHUMAN; PROTEIN LOCALIZATION; PROTEIN METABOLISM; PROTEIN STABILITY; RAT; TOXICITY TESTING; WILD TYPE; ANIMAL; ANTHRAX; CHEMISTRY; CHO CELL; CRICETULUS; FISCHER 344 RAT; HAMSTER; METABOLISM; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; SIGNAL TRANSDUCTION;

ANIMALIA;

AMINO ACIDS; ANIMALS; ANTHRAX; ANTIGENS, BACTERIAL; BACTERIAL TOXINS; CHO CELLS; CRICETINAE; CRICETULUS; FACTOR XA; FEMALE; MAP KINASE SIGNALING SYSTEM; MICE; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RATS; RATS, INBRED F344; ZINC;

EID: 51549119044     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0003130     Document Type: Article

References (41)

1. Mourez M (2004) Anthrax toxins. Rev Physiol Biochem Pharmacol 152: 135-164.
2. Leppla SH (2006) Bacillus anthracis toxins. In: Alouf JE, Popoff MR, eds. The Comprehensive Sourcebook of Bacterial Protein Toxins. Burlington, MA: Academic Press. pp 323-347.
3. Leppla. SH (1982) Anthrax toxin edema factor: a bacterial adenylate cyclase that increases cyclic AMIP concentrations of eukaryotic cells. Proc Natl Acad Sci U S A 79: 3162-3166.
4. Duesbery NS, Webb CP, Leppla SH, Gordon VM, Klimpel KR, et al. (1998) Proteolytic inactivation of MAP-kinase-hinase by anthrax lethal factor. Science 280: 734-737.
5. Pellizzari R, Guidi-Rontani C, Vitale G, Mock M, Montecucco C (2000) Lethal factor of Bacillus anthracis cleaves the N-terminus of MAPKKs: analysis of the intracellular consequences in macrophages. Int J Med Microbiol 290: 421-427.
6. Moayeri M, Haines D, Young HA, Leppla SH (2003) Bacillus anthracis lethal toxin induces TNF-a-independent hypoxia-mediated toxicity in mice. J Clin Invest 112: 670-682.
7. Turk BE (2007) Manipulation of host signalling pathways by anthrax toxins. Biochem J 402: 405-417.
8. Warfel JM, Steele AD, D'Agnillo F (2005) Anthrax lethal toxin induces endothelial barrier dysfunction. Am J Pathol 166: 1871-1881.
9. Webster JI, Tonelli LH, Moayeri M, Simons SS Jr, Leppla SH, et al. (2003) Anthrax lethal factor represses glucocorticoid and progesterone receptor activity. Proc Natl Acad Sci U S A 100: 5706-5711.
10. Friedlander AM (1986) Macrophages are sensitive to anthrax lethal toxin through an acid-dependent process. J Biol Chem 261: 7123-7126.
11. Park JM, Greten FR, Li ZW, Karin M (2002) Macrophage apoptosis by anthrax lethal factor through p38 MAP kinase Inhibition. Science 297: 2048-2051.
12. Gupta P, Batra S, Chopra AP, Singh Y, Bhatnagar R (1998) Expression and purification of the recombinant lethal factor of Bacillusa anthracis. Infect Immun 66: 862-865.
13. Baillie L, Moir A, Manchee R (1998) The expression of the protective antigen of Bacillus anthracis in Bacillus subtilis. J Appl Microbiol 84: 741-746.
14. Kim J, Kim YM, Koo BS, Chae YK, Yoon MY (2003) Production and proteolytic assay of lethal factor from Bacillus anthracis. Protein Expr Purif 30: 293-300.
15. Leppla SH (1988) Production and purification of anthrax toxin. Methods Enzymol 165: 103-116.
16. Leppla SH (1991) Purification and characterization of adenylyl cyclase from Bacillus anthracis. Methods Enzymol 195: 153-168.
17. Singh Y, Chaudhary VK, Leppla SH (1989) A deleted variant of Bacillus anthracis protective antigen is non-toxic and blocks anthrax toxin action in vivo. J Biol Chem 264: 19103-19107.
18. Park S, Leppla SH (2000) Optimized production and purification of Bacillus anthracis lethal factor. Protein Expr Purif 18: 293-302.
19. Wesche J, Elliott JL, Falnes PO, Olsnes S, Collier RJ (1998) Characterization of membrane translocation by anthrax protective antigen. Biochemistry 37: 15737-15746.
20. Varshavsky A (1997) The N-end rule pathway of protein degradation. Genes Cells 2: 13-28.
21. Tasaki T, Kwon YT (2007) The mammalian N-end rule pathway: new insights into its components and physiological roles. Trends Biochem Sci 32: 520-528.
22. Bachmair A, Varshavsky A (1989) The degradation signal in a short-lived protein. Cell 56: 1019-1032.
23. London E, Luongo CL (1989) Domain-specific bias in arginine/lysine usage by protein toxins. Biochem Biophys Res Common 160: 333-339.
24. Liu S, Leung HJ, Leppla SH (2007) Characterization of the interaction between anthrax toxin and its cellular receptors. Cell Microbiol 9: 977-987.
25. Malatynska E, Crites G, Yochum A, Kopp R, Giroux ML, et al. (1998) Schild regression analysis of antidepressant and bicuculline antagonist effects at the GABAA receptor, Pharmacology 57: 117-123.
26. Varughese M, Chi A, Teixeira AV, Nicholls PJ, Keith JM, et al. (1998) Internalization of a Bacillus anthracis protective antigrn-c-Myc fusion protein mediated by cell surface anti-c-Myc antibodies. Mol Med 4: 87-95.
27. Gupta PK, Liu S, Batavia MP, Leppla SH (2008) The diphthamide modification on elongation factor-2 renders mammalian cells resistant to ricin. Cell Microbiol 10: 1687-1694.
28. Gordon VM, Leppla SH, Hewlett EL (1988) Inhibitors of receptor-mediated endocytosis block the entry of Bacillus anthracis adenylate cyclase toxin but not that of Bardetella pertussis adenylate cyclase toxin. Infect Immun 56: 1066-1069.
29. Rainey GJ, Wigelsworth DJ, Ryan PL, Scobie HM, Collier RJ, et al. (2005) Receptor-specific requirements for anthrax toxin delivery into cells. Proc Natl Acad Sci U S A 102: 13278-13283.
30. Wright GG, Puziss M, Neely WB (1962) Studies on immunity in anthrax. IX. Effect of variations in cultural conditions on elaboration of protective antigen by strains of Bacillus anthracis. J Bacteriol 83: 515-522.
31. Gonda DK, Bachmair A, Wunning I, Tobias JW, Lane WS, et al. (1989) Universality and structure of the N-end rule. J Biol Chem 264: 16700-16712.
32. Falnes PO, Olsnes S (1998) Modulation of the intracellular stability and toxicity of diphtheria toxin through degradation by the N-end rule pathway. EMBO J 17: 615-625.
33. Meinnel T, Screro A, Giglione C (2006) Impact of the N-terminal amino acid on targeted protein degradation. Biol Chem 387: 839-851.
34. Ezzell JW, Ivins BE, Leppla SH (1984) Immunoelectrophoretic analysis, toxicity, and kinetics of in vitro production of the protective antigen and lethal factor components of Bacillus anthracis toxin. Infect Immun 45: 761-767.
35. Agaisse H, Gominet M, Okstad OA, Kolsto AB, Lereclus D (1999) PlcR is a pleiotropic regulator of extracellular vindence factor gene expression in Bacillus thuringiensis. Mol Microbiol 32: 1043-1053.
36. Pomerantsev AP, Pomerantseva OM, Leppla SH (2004) A spontaneous translational fusion of Bacillus cereus PlcR and PapR activates transcription of PlcR-dependent genes in Bacillus anthracis via binding with a specific palindromic sequence. Infect Immun 72: 5814-5823.
37. Hu RG, Sheng J, Qi X, Xu Z, Takahashi TT, et al. (2005) The N-end role pathway as a nitric oxide sensor controlling the levels of multiple regulators. Nature 437: 981-986.
38. Schnupf P, Zhou J, Varshavsky A, Portnoy DA (2007) Listeriolysin O secreted by Listeria monocylogenes into the host cell cytosol is degraded by the N-end rule pathway. Infect Immun 75: 5135-5147.
39. Lacy DB, Mourez M, Fouassier A, Collier RJ (2002) Mapping the anthrax protective antigen binding site on the lethal and edema factors. J Biol Chem 277: 3006-3010.
40. Melnyk RA, Hewitt KM, LaLy DB, Lin HC, Gessner CR, et al. (2006) Structural determinants for the binding of anthrax lethal factor to oligameric protective antigen. J Biol Chem 281: 1630-1635.
41. Zhang S, Finkelstein A, Collier RJ (2004) Evidence that translocation of anthrax toxin's lethal factor is initiated by entry of its N terminus into the protective antigen channel. Proc Natl Acad Sci U S A 101: 16756-16761.