Internalization of a Bacillus anthracis protective antigen-c-Myc fusion protein mediated by cell surface anti-c-Myc antibodies

Molecular Medicine

Volumn 4, Issue 2, 1998, Pages 87-95

  Varughese, Mini ^a   Chi, Angela ^a,b   Teixeira, Avelino V ^a,c   Nicholls, Peter J ^a,d   Keith, Jerry M ^a   Leppla, Stephen H ^a  

^a NATIONAL INSTITUTE OF DENTAL AND CRANIOFACIAL RESEARCH   (United States)

^b HARVARD SCHOOL OF DENTAL MEDICINE   (United States)

^c MOUNT SINAI SCHOOL OF MEDICINE   (United States)

^d UNIVERSITY OF KENT   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANTHRAX TOXIN;

ARTICLE; BACILLUS ANTHRACIS; CELL DEATH; CELL KILLING; CELL MEDIATED CYTOTOXICITY; CELL PROLIFERATION; CELL PROTECTION; COMPETITIVE INHIBITION; DRUG RECEPTOR BINDING; ENDOCYTOSIS; GENE TARGETING; INTERNALIZATION; NONHUMAN; ONCOGENE C MYC; PRIORITY JOURNAL;

EID: 0031925706     PISSN: 10761551     EISSN: None     Source Type: Journal    
DOI: 10.1007/bf03401732     Document Type: Article

References (41)

1. Smith H, Keppie J, Stanley JL (1955) The chemical basis of the virulence of Bacillus anthracis. V. The specific toxin produced by B. anthracis in vivo. Br. J. Exp. Pathol. 36: 460-472.
2. Klimpel KR, Molloy SS, Thomas G, Leppla SH (1992) Anthrax toxin protective antigen is activated by a cell-surface protease with the sequence specficitiy and catalytic properties of furin. Proc. Natl. Acad. Sci. U.S.A. 89: 10277-10281.
3. Friedlander AM (1986) Macrophages are sensitive to anthrax lethal toxin through an acid-dependent process. J. Biol. Chem. 261: 7123-7126.
4. Gordon VM, Leppla SH, Hewlett EL (1988) Inhibitors of receptor-mediated endocytosis block the entry of Bacillus anthracis adenylate cyclase toxin but not that of Bordetella pertussis adenylate cyclase toxin. Infect. Immun. 56: 1066-1069.
5. Milne JC, Furlong D, Hanna PC, Wall JS, Collier RJ (1994) Anthrax protective antigen forms oligomers during intoxication of mammalian cells. J. Biol. Chem. 269: 20607-20612.
6. Leppla SH (1991) The anthrax toxin complex. In: (ed Alouf JE, Freer JH) Sourcebook of Bacterial Protein Toxins. Academic Press, London, pp. 277-302.
7. Leppla S (1995) Anthrax toxins. In: (ed Moss J, Iglewski B, Vaughan M, Tu A) Bacterial Toxins and Virulence Factors in Disease. Handbook of Natural Toxins. Vol. 8 Marcel Dekker, New York, pp. 543-572.
8. Beall FA, Taylor MJ, Thorne CB (1962) Rapid lethal effect in rats of a third component found upon fractionating the toxin of Bacillus anthracis. J. Bacterial. 83: 1274-1280.
9. Ezzell JW, Ivins BE, Leppla SH (1984) Immunoelectrophoretic analysis, toxicity, and kinetics of in vitro production of the protective antigen and lethal factor components of Bacillus anthracis toxin. Infect. Immun. 45: 761-767.
10. Hanna PC, Kouchi S, Collier RJ (1992) Biochemical and physiological changes induced by anthrax lethal toxin in J774 macrophage-like cells. Mol. Biol. Cell 3: 1269-1277.
11. Singh Y, Klimpel KR, Quinn CP, Chaudhary VK, Leppla SH (1991) The carboxyl-terminal end of protective antigen is required for receptor binding and anthrax toxin activity. J. Biol. Chem. 266: 15493-15497.
12. Little SF, Leppla SH, Cora E (1988) Production and characterization of monoclonal antibodies to the protective antigen component of Bacillus anthracis toxin. Infect. Immun. 56: 1807-1813.
13. Perelle S, Gibert M, Boquet P, Popoff MR (1993) Characterization of Clostridium perfringens iotatoxin genes and expression in Escherichia coli. Infect. Immun. 61: 5147-5156.
14. Petosa C, Collier RJ, Klimpel KR, Leppla SH, Liddington RC (1997) Crystal structure of the anthrax toxin protective antigen. Nature 385: 833-838.
15. Ramsay G, Evan GI, Bishop JM (1984) The protein encoded by the human proto-oncogene c-myc. Proc. Natl. Acad. Sci. U.S.A. 81: 7742-7746.
16. Kolodziej PA, Young RA (1991) Epitope tagging and protein surveillance. Methods Enzymol. 194: 508-519.
17. Evan GI, Lewis GK, Ramsay G, Bishop JM (1985) Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell. Biol 5: 3610-3616.
18. Koskinen PJ, Alitalo K (1993) Role of myc amplification and overexpression in cell growth, differentiation and death. Semin. Cancer Biol. 4: 3-12.
19. Vastrik I, Makela TP, Koskinen PJ, Klefstrom J, Alitalo K (1994) Myc protein: Partners and antagonists. Crit. Rev. Oncog. 5: 59-68.
20. Ryan KM, Birnie GD (1996) Myc oncogenes: The enigmatic family. Biochem. J. 314: 713-721.
21. DeFranco AL (1992) Tyrosine phosphorylation and the mechanism of signal transduction by the B-ymphocyte antigen receptor. Eur. J. Biochem. 210: 381-388.
22. Reth M (1992) Antigen receptors on B lymphocytes. Annu. Rev. Immunol. 10: 97-121.
23. Reth M, Hombach J, Wienands J, Campbell KS, Chien N, Justement LB, Cambier JC (1991) The B-cell antigen receptor complex. Immunol. Today 12: 196-201.
24. Singh Y, Chaudhary VK, Leppla SH (1989) A deleted variant of Bacillus anthracis protective antigen is non-toxic and blocks anthrax toxin action in vivo. J. Biol. Chem. 264: 19103-19107.
25. Welkos SL, Lowe JR, Eden-McCutchan F, Vodkin M, Leppla SH, Schmidt JJ (1988) Sequence and analysis of the DNA encoding protective antigen of Bacillus anthracis. Gene 69: 287-300.
26. Quinn CP, Dancer BN (1990) Transformation of vegetative cells of Bacillus anthracis with plasmid DNA. J. Gen. Microbiol. 136: 1211-1215.
27. Shulman M, Wilde CD, Kohler G (1978) A better cell line for making hybridomas secreting specific antibodies. Nature 276: 269-270.
28. Quinn CP, Singh Y, Klimpel KR, Leppla SH (1991) Functional mapping of anthrax toxin lethal factor by in-frame insertion mutagenesis. J. Biol. Chem. 266: 20124-20130.
29. Scudiero DA, Shoemaker RH, Paull KD, Monks A, Tierney S, Nofziger TH, Currens MJ, Seniff D, Boyd MR (1988) Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res. 48: 4827-4833.
30. Arora N, Klimpel KR, Singh Y, Leppla SH (1992) Fusions of anthrax toxin lethal factor to the ADP-ribosylation domain of Pseudomonas exotoxin A are potent cytotoxins which are translocated to the cytosol of mammalian cells. J. Biol. Chem. 267: 15542-15548.
31. Arora N, Leppla SH (1993) Residues 1-254 of anthrax toxin lethal factor are sufficient to cause cellular uptake of fused polypeptides. J. Biol. Chem. 268: 3334-3341.
32. Leppla SH (1988) Production and purification of anthrax toxin. Methods Enzymol. 165: 103-116.
33. Madshus IH, Stenmark H, Sandvig K, Olsnes S (1991) Entry of diphtheria toxin-protein A chimeras into cells. J. Biol. Chem. 266: 17446-17453.
34. Vallera DA, Ash RC, Zanjani ED, Kersey JH, LeBien TW, Beverley PC, Neville, Jr, DM, Youle RJ (1983) Anti-T-cell reagents for human bone marrow transplantation: Ricin linked to three monoclonal antibodies. Science 222: 512-515.
35. Chaudhary VK, Queen C, Junghans RP, Waldmann TA, FitzGerald DJ, Pastan I (1989) A recombinant immunotoxin consisting of two antibody variable domains fused to Pseudomonas exotoxin. Nature 339: 394-397.
36. Fisher CE, Sutherland JA, Krause JE, Murphy JR, Leeman SE, vanderSpek JC (1996) Genetic construction and properties of a diphtheria toxin-related substance P fusion protein: In vitro destruction of cells bearing substance P receptors. Proc. Natl. Acad. Sci. U.S.A. 93: 7341-7345.
37. FitzGerald D, Pastan I (1991) Redirecting Pseudomonas exotoxin. Semin. Cell Biol. 2: 31-37.
38. Arora N, Williamson LC, Leppla SH, Halpern JL (1994) Cytotoxic effects of a chimeric protein consisting of tetanus toxin light chain and anthrax toxin lethal factor in non-neuronal cells. J. Biol. Chem. 269: 26165-26171.
39. Arora N, Leppla SH (1994) Fusions of anthrax toxin lethal factor with shiga toxin and diphtheria toxin enzymatic domains are toxic to mammalian cells. Infect. Immun. 62: 4955-4961.
40. Milne JC, Blanke SR, Hanna PC, Collier RJ (1995) Protective antigen-binding domain of anthrax lethal factor mediates translocation of a heterologous protein fused to its amino-or carboxy-terminus. Mol. Microbiol. 15: 661-666.
41. Leppla SH, Kumpel KR, Arora N (1994) Development of anthrax toxin-based fusion proteins for targeting of HIV-1-infected cells. In: Fehrenbach F (ed). Bacterial Protein Toxins. Gustav Fischer Verlag, Stuttgart, pp. 431-442.