A novel auto-cleavage assay for studying mutational effects on the active site of severe acute respiratory syndrome coronavirus 3C-like protease

Biochemical and Biophysical Research Communications

Volumn 324, Issue 2, 2004, Pages 579-583

  Shan, Yu Fei ^a   Li, Shou Feng ^a   Xu, Gen Jun ^a,b  

^a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

^b ZheJiang Sci University   (China)

Author keywords

3CL protease; Active site; Auto cleavage assay; N 31 tag; SARS

Indexed keywords

CYSTEINE; HISTIDINE; PROTEINASE; VIRUS ENZYME;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL MUTATION; CATALYSIS; CORONAVIRUS; DRUG DESIGN; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; SEVERE ACUTE RESPIRATORY SYNDROME;

BINDING SITES; CATALYTIC DOMAIN; CYSTEINE ENDOPEPTIDASES; DNA MUTATIONAL ANALYSIS; DNA PRIMERS; DOSE-RESPONSE RELATIONSHIP, DRUG; ENDOPEPTIDASES; ESCHERICHIA COLI; HISTIDINE; MUTAGENESIS, SITE-DIRECTED; MUTATION; PEPTIDES; PROTEIN STRUCTURE, TERTIARY; SERINE; TIME FACTORS; VIRAL PROTEINS;

CORONAVIRUS; ESCHERICHIA COLI;

EID: 5144224617     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2004.09.088     Document Type: Article

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