Expression and characterization of a recombinant murine coronavirus 3C-like proteinase

Journal of General Virology

Volumn 78, Issue 1, 1997, Pages 71-75

  Seybert, Anja ^a   Ziebuhr, John ^a   Siddell, Stuart G ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEINASE; PROTEINASE INHIBITOR; VIRUS ENZYME;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; CORONAVIRUS; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME STRUCTURE; NONHUMAN; PICORNAVIRUS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; SEQUENCE HOMOLOGY; STRUCTURE ACTIVITY RELATION; VIRUS RECOMBINANT;

BACTERIA (MICROORGANISMS); CORONAVIRUS; MURINAE; MURINE HEPATITIS VIRUS; PICORNAVIRIDAE;

EID: 0031013797     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/0022-1317-78-1-71     Document Type: Article

References (20)

1. Baum, E. Z., Bebernitz, G. A., Palant, O., Mueller, T. & Plotch, S. J. (1991). Purification, properties, and mutagenesis of poliovirus 3C protease. Virology 185, 140-150.
2. Boursnell, M. E. G., Brown, T. D. K., Foulds, I. J., Green, P. F., Tomley, F. M. & Binns, M. M. (1987). Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus. Journal of General Virology 68, 57-77.
3. Bredenbeek, P. J., Pachuk, C. J., Noten, A. F. H., Charité, J., Luytjes, W., Weiss, S. R. & Spaan, W. J. M. (1990). The primary structure and expression of the second open reading frame of the polymerase gene of the coronavirus MHV-A59; a highly conserved polymerase is expressed by an efficient ribosomal frameshifting mechanism. Nucleic Acids Research 18, 1825-1832.
4. Brierley, I., Boursnell, M. E. G., Binns, M. M., Billimoria, B., Blok, V. C., Brown, T. D. K. & Inglis, S. C. (1987). An efficient ribosomal frameshifting signal in the polymerase-encoding region of the coronavirus IBV. EMBO Journal 6, 3779-3785.
5. Brierley, I., Digard, P. & Inglis, S. C. (1989). Characterization of an efficient coronavirus ribosomal frameshift signal: requirement for an RNA pseudoknot. Cell 57, 537-547.
6. Eleouet, J.-F, Rasschaert, D., Lambert, P., Levy, L., Vende, P. & Laude, H. (1995). Complete sequence (20 kilobases) of the polyprotein-encoding gene 1 of transmissible gastroenteritis virus. Virology 206, 817-822.
7. Gorbalenya, A. E., Koonin, E. V., Donchenko, A. P. & Blinov, V. M. (1989). Coronavirus genome: prediction of putative functional domains in the nonstructural polyprotein by comparative amino acid sequence analysis. Nucleic Acids Research 17, 4847-4861.
8. Grötzinger, C., Heusipp, G., Ziebuhr, J., Harms, U., Süss, J. & Siddell, S. G. (1996). Characterization of a 105-kDa polypeptide encoded in gene 1 of the human coronavirus 229E. Virology 222, 227-235.
9. Herold, J. & Siddell, S. G. (1993). An elaborated pseudoknot is required for high frequency frameshifting during translation of HCV 229E polymerase mRNA. Nucleic Acids Research 21, 5838-5842.
10. Herold, J., Raabe, T., Schelle-Prinz, B. & Siddell, S. G. (1993). Nucleotide sequence of the human coronavirus 229E RNA polymerase locus. Virology 195, 680-691.
11. Herold, J., Siddell, S. G. & Ziebuhr, J. (1996). Characterization of coronavirus RNA polymerase gene products. Methods in Enzymology 275 (in press).
12. Lee, H.-J., Shieh, C.-K., Gorbalenya, A. E., Koonin, E. V., La Monica, N., Tuler, J., Bagdzhadzhyan, A. & Lai, M. M. C. (1991). The complete sequence (22 kilobases) of murine coronavirus gene 1 encoding the putative proteases and RNA polymerase. Virology 180, 567-582.
13. Liu, D. X. & Brown, T. D. K. (1995). Characterization and mutational analysis of an ORF 1a-encoding proteinase domain responsible for proteolytic processing of the infectious bronchitis virus 1a/1b polyprotein. Virology 209, 420-427.
14. Liu, D. X., Brierley, I., Tibbles, K. W. & Brown, T. D. K. (1994). A 100-kilodalton polypeptide encoded by open reading frame (ORF) 1b of the coronavirus infectious bronchitis virus is processed by ORF 1a products. Journal of Virology 68, 5772-5780.
15. Lu, Y., Lu, X. & Denison, M. R. (1995). Identification and characterization of a serine-like proteinase of the murine coronavirus MHV-A59. Journal of Virology 69, 3554-3559.
16. Malcolm, B. A., Chin, S. M., Jewell, D. A., Stratton-Thomas, J. R., Thudium, K. B., Ralston, R. & Rosenberg, S. (1992). Expression and characterization of recombinant hepatitis A virus 3C proteinase. Biochemistry 31, 3358-3363.
17. Tibbles, K. W., Brierley, I., Cavanagh, D. & Brown, T. D. K. (1995). A region of the infectious bronchitis virus 1a polyprotein encoding the 3C-like protease domain is subject to rapid turnover when expressed in rabbit reticulocyte lysate. Journal of General Virology 76, 3059-3070.
18. Tibbles, K. W., Brierley, I., Cavanagh, D. & Brown, T. D. K. (1996). Characterization in vitro of an autocatalytic processing activity associated with the predicted 3C-like proteinase domain of the coronavirus avian infectious bronchitis virus. Journal of Virology 70, 1923-1930.
19. Yao, Z., Jones, D. H. & Grose, C. (1992). Site-directed mutagenesis of herpesvirus glycoprotein phosphorylation sites by recombination polymerase chain reaction. PCR Methods and Applications 1, 205-207.
20. Ziebuhr, J., Herold, J. & Siddell, S. G. (1995). Characterization of a human coronavirus (strain 229E) 3C-like proteinase activity. Journal of Virology 69, 4331-4338.