Distinct contributions of the nisin biosynthesis enzymes NisB and NisC and transporter NisT to prenisin production by Lactococcus lactis

Applied and Environmental Microbiology

Volumn 74, Issue 17, 2008, Pages 5541-5548

  Van Bart Den Berg Van Saparoea, H ^a   Bakkes, Patrick J ^a   Moll, Gert N ^a   Driessen, Arnold J M ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

AMINES; BIOCHEMICAL ENGINEERING; BIOCHEMISTRY; CATALYSTS; ENZYMES; PROTEINS;

CYCLASE; LACTOCOCCUS LACTIS; NISIN PRODUCTION; THIOETHER;

ANTIBIOTICS;

NISIN; PEPTIDE DERIVATIVE; PRENISIN; PROTEIN DERIVATIVE; PROTEIN NISB; PROTEIN NISC; UNCLASSIFIED DRUG;

BACTERIUM; ENZYME; KINETICS; PEPTIDE;

ARTICLE; ENZYME KINETICS; ENZYME SYNTHESIS; GROWTH RATE; LACTOCOCCUS LACTIS; NONHUMAN; PROTEIN MODIFICATION; PROTEIN SECRETION; PROTEIN SYNTHESIS; PROTEIN TRANSPORT;

BACTERIAL PROTEINS; BACTERIOCINS; BIOLOGICAL TRANSPORT; GENES, BACTERIAL; LACTOCOCCUS LACTIS; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; NISIN; PROTEIN PRECURSORS;

LACTOCOCCUS LACTIS;

EID: 50949089673     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.00342-08     Document Type: Article

References (32)

1. Chatterjee, C., M. Paul, L. Xie, and W. A. van der Denk. 2005. Biosynthesis and mode of action of lantibiotics. Chem. Rev. 105:633-684.
2. Engelke, G., Z. Gutowski-Eckel, M. Hammelmann, and K. D. Entian. 1992. Biosynthesis of the lantibiotic nisin: genomic organization and membrane localization of the NisB protein. Appl. Environ. Microbiol. 58:3730-3743.
3. Engelke, G., Z. Gutowski-Eckel, P. Kiesau, K. Siegers, M. Hammelmann, and K. D. Entian. 1994. Regulation of nisin biosynthesis and immunity in Lactococcus lactis 6F3. Appl. Environ. Microbiol. 60:814-825.
4. Gross, E., and J. L. Morell. 1971. The structure of nisin. J. Am. Chem. Soc. 93:4634-1635.
5. Hasper, H. E., N. E. Kramer, J. L. Smith, J. D. Hillman, C. Zachariah, O. P. Kuipers, B. de Kruijff, and E. Breukink. 2006. An alternative bactericidal mechanism of action for lantibiotic peptides that target lipid II. Science 313:1636-1637.
6. Kaletta, C, and K. D. Entian. 1989. Nisin, a peptide antibiotic: cloning and sequencing of the nisA gene and posttranslational processing of its peptide product. J. Bacteriol. 171:1597-1601.
7. Kluskens, L. D., A. Kuipers, R. Rink, E. de Boef, S. Fekken, A. J. M. Driessen, O. P. Kuipers, and G. N. Moll. 2005. Post-translational modification of therapeutic peptides by NisB, the dehydratase of the lantibiotic nisin. Biochemistry 44:12827-12834.
8. Kuipers, A., E. de Boef, R. Rink, S. Fekken, L. D. Kluskens, A. J. M. Driessen, K. Leenhouts, O. P. Kuipers, and G. N. Moll. 2004. NisT, the transporter of the lantibiotic nisin, can transport fully modified, dehydrated, and unmodified prenisin and fusions of the leader peptide with non-lantibiotic peptides. J. Biol. Chem. 279:22176-22182.
9. Kuipers, A., J. Wierenga, R. Rink, L. D. Kluskens, A. J. M. Driessen, O. P. Kuipers, and G. N. Moll. 2006. Sec-mediated transport of posttranslationally dehydrated peptides in Lactococcus lactis. Appl. Environ. Microbiol. 72:7626-7633.
10. Kuipers, O. P., M. M. Beerthuyzen, P. G. de Ruyter, E. J. Luesink, and W. M. de Vos. 1995. Autoregulation of nisin biosynthesis in Lactococcus lactis by signal transduction. J. Biol. Chem. 270:27299-27304.
11. Kuipers, O. P., M. M. Beerthuyzen, R. J. Siezen, and W. M. de Vos. 1993. Characterization of the nisin gene cluster nisABTCIPR of Lactococcus lactis. Requirement of expression of the nisA and nisI genes for development of immunity. Eur. J. Biochem. 216:281-291.
12. Kuipers, O. P., P. G. G. A. de Ruyter, M. Kleerebezem, and W. M. de Vos. 1998. Quorum sensing-controlled gene expression in lactic acid bacteria. J. Biotechnol. 64:15-21.
13. Li, B., and W. A. van der Denk. 2007. Identification of essential catalytic residues of the cyclase NisC involved in the biosynthesis of nisin. J. Biol. Chem. 282:21169-21175.
14. Li, Y., J. Hugenholtz, T. Abee, and D. Molenaar. 2003. Glutathione protects Lactococcus lactis against oxidative stress. Appl. Environ. Microbiol. 69:5739-5745.
15. Mattick, A. T. R., A. Hirsch, and N. J. Berridge. 1947. Further observations on an inhibitory substance (nisin) from lactic streptococci. Lancet ii:5-8.
16. Mierau, I., and M. Kleerebezem. 2005. 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis. Appl. Microbiol. Biotechnol. 68:705-717.
17. Qiao, M., and P. E. Saris. 1996. Evidence for a role of NisT in transport of the lantibiotic nisin produced by Lactococcus lactis N8. FEMS Microbiol. Lett. 144:89-93.
18. Ra, R., M. M. Beerthuyzen, W. M. de Vos, P. E. Saris, and O. P. Kuipers. 1999. Effects of gene disruptions in the nisin gene cluster of Lactococcus lactis on nisin production and producer immunity. Microbiology 145:1227-1233.
19. Ra, R., M. Qiao, T. Immonen, I. Pujana, and P. E. J. Saris. 1996. Genes responsible for nisin synthesis, regulation and immunity form a regulon of two operons and are induced by nisin in Lactoccocus lactis N8. Microbiology 142:1281-1288.
20. Rink, R., A. Kuipers, E. de Boef, K. J. Lienhouts, A. J. M. Driessen, G. N. Moll, and O. P. Kuipers. 2005. Lantibiotic structures as guidelines for the design of peptides that can be modified by lantibiotic enzymes. Biochemistry 44:8873-8882.
21. Rink, R., J. Wierenga, A. Kuipers, L. D. Kluskens, A. J. M. Driessen, O. P. Kuipers, and G. N. Moll. 2007. Dissection and modulation of the four distinct activities of nisin by mutagenesis of rings A and B and by C-terminal truncation. Appl. Environ. Microbiol. 73:5809-5816.
22. Rink, R., J. Wierenga, A. Kuipers, L. D. Kluskens, A. J. M. Driessen, O. P. Kuipers, and G. N. Moll. 2007. Production of dehydroamino acid-containing peptides by Lactococcus lactis. Appl. Environ. Microbiol. 73:1792-1796.
23. Rogers, L. A., and E. O. Whittier. 1928. Limiting factors in the lactic fermentation. J. Bacteriol. 16:211-229.
24. Rollema, H. S., J. W. Metzger, P. Both, O. P. Kuipers, and R. J. Siezen. 1996. Structure and biological activity of chemically modified nisin A species. Eur. J. Biochem. 241:716-722.
25. Schägger, H. 2006. Tricine-SDS-PAGE. Nat. Protoc. 1:16-22.
26. Schnell, N., K. D. Entian, U. Schneider, F. Gotz, H. Zahner, R. Kellner, and G. Jung. 1988. Prepeptide sequence of epidermin, a ribosomally synthesized antibiotic with four sulphide-rings. Nature 333:276-278.
27. Siegers, K., and K. D. Entian. 1995. Genes involved in immunity to the lantibiotic nisin produced by Lactococcus lactis 6F3. Appl. Environ. Microbiol. 61:1082-1089.
28. Siegers, K., S. Heinzmann, and K. D. Entian. 1996. Biosynthesis of lantibiotic nisin. Posttranslational modification of its prcpeptidc occurs at a multimeric membrane-associated lanthionine synthetase complex. J. Biol. Chem. 271:12294-12301.
29. Takala, T. M., and P. E. Saris. 2006. C terminus of Nisi provides specificity to nisin. Microbiology 152:3543-3549.
30. van der Meer, J. R., J. Polman, M. M. Beerthuyzen, R. J. Siezen, O. P. Kuipers, and W. M. de Vos. 1993. Characterization of the Lactococcus lactis nisin A Operon genes nisP, encoding a subtilisin-like serine protease involved in precursor processing, and nisR, encoding a regulatory protein involved in nisin biosynthesis. J. Bacteriol. 175:2578-2588.
31. Reference deleted.
32. Zwietering, M. H., I. Jongenburger, F. M. Rombouts, and K. van't Riet. 1990. Modeling of the bacterial growth curve. Appl. Environ. Microbiol. 56:1875-1881.