A TLR4/MD2 fusion protein inhibits LPS-induced pro-inflammatory signaling in hepatic stellate cells

Biochemical and Biophysical Research Communications

Volumn 375, Issue 2, 2008, Pages 210-214

  Schnabl, Bernd ^a   Brandl, Katharina ^b   Fink, Marina ^b   Gross, Philipp ^b   Taura, Kojiro ^a   Gäbele, Erwin ^b   Hellerbrand, Claus ^b   Falk, Werner ^b  

^a UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

^b UNIVERSITY OF REGENSBURG   (Germany)

Author keywords

Hepatic stellate cells; Liver fibrosis; LPS; Toll like receptor

Indexed keywords

HYBRID PROTEIN; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; IMMUNOGLOBULIN FC FRAGMENT; INTERLEUKIN 6; INTERLEUKIN 8; LIPOPOLYSACCHARIDE; MONOCYTE CHEMOTACTIC PROTEIN 1; PROTEIN MD 2; RANTES; SCLEROPROTEIN; STRESS ACTIVATED PROTEIN KINASE; TLR4 MD2 PROTEIN; TOLL LIKE RECEPTOR 4; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; FIBROGENESIS; HUMAN; HUMAN CELL; INFLAMMATION; LIVER FIBROSIS; LIVER INJURY; PRIORITY JOURNAL; STELLATE CELL;

ANIMALS; CELLS, CULTURED; CHEMOKINES; HUMANS; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; LIPOPOLYSACCHARIDES; LIVER CIRRHOSIS; LYMPHOCYTE ANTIGEN 96; MAP KINASE KINASE 4; MICE; NF-KAPPA B; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SIGNAL TRANSDUCTION; TOLL-LIKE RECEPTOR 4;

MUS;

EID: 50249108974     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2008.07.150     Document Type: Article

References (26)

1. Bataller R., and Brenner D.A. Liver fibrosis. J. Clin. Invest. 115 (2005) 209-218
2. Akira S., Uematsu S., and Takeuchi O. Pathogen recognition and innate immunity. Cell 124 (2006) 783-801
3. Schromm A.B., Lien E., Henneke P., Chow J.C., Yoshimura A., Heine H., Latz E., Monks B.G., Schwartz D.A., Miyake K., and Golenbock D.T. Molecular genetic analysis of an endotoxin nonresponder mutant cell line: a point mutation in a conserved region of MD-2 abolishes endotoxin-induced signaling. J. Exp. Med. 194 (2001) 79-88
4. Ohto U., Fukase K., Miyake K., and Satow Y. Crystal structures of human MD-2 and its complex with antiendotoxic lipid IVa. Science 316 (2007) 1632-1634
5. Huang H., Shiffman M.L., Friedman S., Venkatesh R., Bzowej N., Abar O.T., Rowland C.M., Catanese J.J., Leong D.U., Sninsky J.J., Layden T.J., Wright T.L., White T., and Cheung R.C. A 7 gene signature identifies the risk of developing cirrhosis in patients with chronic hepatitis C. Hepatology 46 (2007) 297-306
6. Wiest R., and Garcia-Tsao G. Bacterial translocation (BT) in cirrhosis. Hepatology 41 (2005) 422-433
7. Isayama F., Hines I.N., Kremer M., Milton R.J., Byrd C.L., Perry A.W., McKim S.E., Parsons C., Rippe R.A., and Wheeler M.D. LPS signaling enhances hepatic fibrogenesis caused by experimental cholestasis in mice. Am J Physiol Gastrointest Liver Physiol 290 (2006) G1318-G1328
8. Seki E., De Minicis S., Osterreicher C.H., Kluwe J., Osawa Y., Brenner D.A., and Schwabe R.F. TLR4 enhances TGF-beta signaling and hepatic fibrosis. Nat. Med. 13 (2007) 1324-1332
9. Paik Y.H., Schwabe R.F., Bataller R., Russo M.P., Jobin C., and Brenner D.A. Toll-like receptor 4 mediates inflammatory signaling by bacterial lipopolysaccharide in human hepatic stellate cells. Hepatology 37 (2003) 1043-1055
10. Sprenger H., Kaufmann A., Garn H., Lahme B., Gemsa D., and Gressner A.M. Differential expression of monocyte chemotactic protein-1 (MCP-1) in transforming rat hepatic stellate cells. J. Hepatol. 30 (1999) 88-94
11. Brun P., Castagliuolo I., Pinzani M., Palu G., and Martines D. Exposure to bacterial cell wall products triggers an inflammatory phenotype in hepatic stellate cells. Am. J. Physiol. Gastrointest. Liver Physiol. 289 (2005) G571-G578
12. Brandl K., Gluck T., Hartmann P., Salzberger B., and Falk W. A designed TLR4/MD-2 complex to capture LPS. J. Endotoxin Res. 11 (2005) 197-206
13. Schnabl B., Bradham C.A., Bennett B.L., Manning A.M., Stefanovic B., and Brenner D.A. TAK1/JNK and p38 have opposite effects on rat hepatic stellate cells. Hepatology 34 (2001) 953-963
14. Tacke F., Gabele E., Bataille F., Schwabe R.F., Hellerbrand C., Klebl F., Straub R.H., Luedde T., Manns M.P., Trautwein C., Brenner D.A., Scholmerich J., and Schnabl B. Bone morphogenetic protein 7 is elevated in patients with chronic liver disease and exerts fibrogenic effects on human hepatic stellate cells. Dig. Dis. Sci. 52 (2007) 3404-3415
15. Schwabe R.F., Schnabl B., Kweon Y.O., and Brenner D.A. CD40 activates NF-kappa B and c-Jun N-terminal kinase and enhances chemokine secretion on activated human hepatic stellate cells. J. Immunol. 166 (2001) 6812-6819
16. Schnabl B., Purbeck C.A., Choi Y.H., Hagedorn C.H., and Brenner D.A. Replicative senescence of activated human hepatic stellate cells is accompanied by a pronounced inflammatory but less fibrogenic phenotype. Hepatology 37 (2003) 653-664
17. Schnabl B., Choi Y.H., Olsen J.C., Hagedorn C.H., and Brenner D.A. Immortal activated human hepatic stellate cells generated by ectopic telomerase expression. Lab. Invest. 82 (2002) 323-333
18. Schnabl B., Kweon Y.O., Frederick J.P., Wang X.F., Rippe R.A., and Brenner D.A. The role of Smad3 in mediating mouse hepatic stellate cell activation. Hepatology 34 (2001) 89-100
19. Schnabl B., Hu K., Muhlbauer M., Hellerbrand C., Stefanovic B., Brenner D.A., and Scholmerich J. Zinc finger protein 267 is up-regulated during the activation process of human hepatic stellate cells and functions as a negative transcriptional regulator of MMP-10. Biochem. Biophys. Res. Commun. 335 (2005) 87-96
20. Muhlbauer M., Weiss T.S., Thasler W.E., Gelbmann C.M., Schnabl B., Scholmerich J., and Hellerbrand C. LPS-mediated NFkappaB activation varies between activated human hepatic stellate cells from different donors. Biochem. Biophys. Res. Commun. 325 (2004) 191-197
21. Chernajovsky Y., Gould D.J., and Podhajcer O.L. Gene therapy for autoimmune diseases: quo vadis?. Nat. Rev. Immunol. 4 (2004) 800-811
22. Schnabl B., Scholten D., and Brenner D.A. What is the potential role of antifibrotic agents for the treatment of liver disease?. Nat. Clin. Pract. Gastroenterol. Hepatol. (2008)
23. Sprenger H., Kaufmann A., Garn H., Lahme B., Gemsa D., and Gressner A.M. Induction of neutrophil-attracting chemokines in transforming rat hepatic stellate cells. Gastroenterology 113 (1997) 277-285
24. Thirunavukkarasu C., Uemura T., Wang L.F., Watkins S.C., and Gandhi C.R. Normal rat hepatic stellate cells respond to endotoxin in LBP-independent manner to produce inhibitor(s) of DNA synthesis in hepatocytes. J. Cell. Physiol. 204 (2005) 654-665
25. Duffield J.S., Forbes S.J., Constandinou C.M., Clay S., Partolina M., Vuthoori S., Wu S., Lang R., and Iredale J.P. Selective depletion of macrophages reveals distinct, opposing roles during liver injury and repair. J. Clin. Invest. 115 (2005) 56-65
26. Imamura M., Ogawa T., Sasaguri Y., Chayama K., and Ueno H. Suppression of macrophage infiltration inhibits activation of hepatic stellate cells and liver fibrogenesis in rats. Gastroenterology 128 (2005) 138-146