Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons

Science

Volumn 321, Issue 5893, 2008, Pages 1218-1221

  Dimos, John T ^a   Rodolfa, Kit T ^a,b   Niakan, Kathy K ^a   Weisenthal, Laurin M ^a   Mitsumoto, Hiroshi ^c,d   Chung, Wendy ^d,e   Croft, Gist F ^d   Saphier, Genevieve ^a   Leibel, Rudy ^e   Goland, Robin ^d   Wichterle, Hynek ^d   Henderson, Christopher E ^d   Eggan, Kevin ^a  

^a Massachusetts General Hospital   (United States)

^b HARVARD UNIVERSITY   (United States)

^c NEUROLOGICAL INSTITUTE   (United States)

^d New York Presbyterian Morgan Stanley Children's Hospital   (United States)

^e Department of Neurology and the Taub Institute for Research on Alzheimer's Disease and the Aging Brain   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOLOGY; NERVOUS SYSTEM;

AGED; AMYOTROPHIC LATERAL SCLEROSIS; ARTICLE; CASE REPORT; CELL CYCLE; CELL DIFFERENTIATION; CELL THERAPY; EMBRYONIC STEM CELL; FEMALE; FIBROBLAST; GENE EXPRESSION; HUMAN; HUMAN CELL; MOTONEURON; PLURIPOTENT STEM CELL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

AGED, 80 AND OVER; AMYOTROPHIC LATERAL SCLEROSIS; CELL DIFFERENTIATION; CELL LINE; EMBRYONIC STEM CELLS; FEMALE; FIBROBLASTS; GENE EXPRESSION; HUMANS; MOTOR NEURONS; NEUROGLIA; NUCLEAR REPROGRAMMING; PLURIPOTENT STEM CELLS; RETROVIRIDAE; SPINAL CORD; SUPEROXIDE DISMUTASE; TRANSCRIPTION FACTORS; TRANSDUCTION, GENETIC;

IPS;

EID: 50149098605     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1158799     Document Type: Article

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27. We thank D. Melton, D. Egli, A. McMahon, K. Osafune, K. Plath, S. Fielding, and K. Hochedlinger for helpful discussions; T. Kitamura, S. Yamanaka, and R. Weinberg for providing viral vectors through Addgene; L. Rubin for generously providing the small-molecule SHH agonist; the Developmental Studies Hybridoma Bank for monoclonal antibodies; and J. Montes and M. Lee for their support in obtaining institutional review board approval and culturing biopsies. This collaboration was supported and enabled by the Project ALS/Jenifer Estess Laboratory for Stem Cell Research, where much of the Columbia research was carried out. Supported by the Harvard Stem Cell Institute, Project ALS, the SMA Foundation, the Claire and Leonard Tow Foundation, MDA Wings Over Wall Street, the Spina and Bowen families, and the New York Stem Cell Foundation. K.T.R. is a National Science Foundation predoctoral fellow, G.F.C. is a Project ALS predoctoral fellow, and K.E. is a fellow of the John D. and Catherine T. MacArthur Foundation.