Identification and characterization of a cathepsin L-like cysteine protease from Gnathostoma spinigerum

Molecular and Biochemical Parasitology

Volumn 160, Issue 2, 2008, Pages 129-137

  Kongkerd, Natthawan ^a   Uparanukraw, Pichart ^a   Morakote, Nimit ^a   Sajid, Mohammed ^b   McKerrow, James H ^b  

^a Faculty of Medicine Chiang Mai University   (Thailand)

^b Sandler Center for Basic Research in Parasitic Diseases   (United States)

Author keywords

Cathepsin L; Cysteine protease; Gnathostoma spinigerum; Gnathostomiasis; Pichia pastoris expression; Recombinant protein

Indexed keywords

CATHEPSIN L; CYSTEINE PROTEINASE; PROTEINASE;

AMINO ACID SEQUENCE; ARTICLE; CAENORHABDITIS ELEGANS; CONTROLLED STUDY; DNA LIBRARY; ENZYME ACTIVITY; GNATHOSTOMA SPINIGERUM; HYDROPHILICITY; INTESTINE CELL; LARVAL STAGE; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PHYLOGENY; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; THELAZIA; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CAENORHABDITIS ELEGANS; CATHEPSINS; CLONING, MOLECULAR; COUMARINS; CYSTEINE ENDOPEPTIDASES; CYSTEINE PROTEINASE INHIBITORS; DIPEPTIDES; GENE EXPRESSION; GENE LIBRARY; GNATHOSTOMA; HUMANS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; OPEN READING FRAMES; PHYLOGENY; PICHIA; PROTEIN SORTING SIGNALS; PROTEIN STRUCTURE, TERTIARY; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

CAENORHABDITIS ELEGANS; GNATHOSTOMA SPINIGERUM; PICHIA PASTORIS;

EID: 50049131642     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2008.05.001     Document Type: Article

References (47)

1. Rusnak J.M., and Lucey D.R. Clinical gnathostomiasis: case report and review of the English-language literature. Clin Infect Dis 16 (1993) 33-50
2. Camacho L.H., Gordeuk V.R., Wilairatana P., et al. The course of anaemia after the treatment of acute, falciparum malaria. Ann Trop Med Parasitol 92 (1998) 525-537
3. Ogata K., Nawa Y., Akahane H., et al. Short report: gnathostomiasis in Mexico. Am J Trop Med Hyg 58 (1998) 316-318
4. Pelaez D., and Perez-Reyes R. Gnathostomiasis in America. Rev Latinoam Microbiol 12 (1970) 83-91
5. Chitanondh H., and Rosen L. Fatal eosinophilic encephalomyelitis caused by the nematode Gnathostoma spinigerum. Am J Trop Med Hyg 16 (1967) 638-645
6. Kawamura J., Kohri Y., and Oka N. Eosinophilic meningoradiculomyelitis caused by Gnathostoma spinigerum. A case report. Arch Neurol 40 (1983) 583-585
7. Punyagupta S., Juttijudata P., Bunnag T., et al. Two fatal cases of eosinophilic myeloencephalitis a newly recognized disease caused by Gnathostoma spinigerum. Trans R Soc Trop Med Hyg 62 (1968) 801-809
8. Berasain P., Goni F., McGonigle S., et al. Proteinases secreted by Fasciola hepatica degrade extracellular matrix and basement membrane components. J Parasitol 83 (1997) 1-5
9. Hotez P., Haggerty J., Hawdon J., et al. Metalloproteases of infective Ancylostoma hookworm larvae and their possible functions in tissue invasion and ecdysis. Infect Immun 58 (1990) 3883-3892
10. McKerrow J.H., and Doenhoff M.J. Schistosome proteases. Parasitol Today 4 (1988) 334-340
11. Chappell C.L., and Dresden M.H. Schistosoma mansoni: proteinase activity of "hemoglobinase" from the digestive tract of adult worms. Exp Parasitol 61 (1986) 160-167
12. Shompole S., and Jasmer D.P. Cathepsin B-like cysteine proteases confer intestinal cysteine protease activity in Haemonchus contortus. J Biol Chem 276 (2001) 2928-2934
13. Berasain P., Carmona C., Frangione B., et al. Fasciola hepatica: parasite-secreted proteinases degrade all human IgG subclasses: determination of the specific cleavage sites and identification of the immunoglobulin fragments produced. Exp Parasitol 94 (2000) 99-110
14. Carmona C., Dowd A.J., Smith A.M., et al. Cathepsin L proteinase secreted by Fasciola hepatica in vitro prevents antibody-mediated eosinophil attachment to newly excysted juveniles. Mol Biochem Parasitol 62 (1993) 9-17
15. Chapman C.B., and Mitchell G.F. Proteolytic cleavage of immunoglobulin by enzymes released by Fasciola hepatica. Vet Parasitol 11 (1982) 165-178
16. Kong Y., Chung Y.B., Cho S.Y., et al. Cleavage of immunoglobulin G by excretory-secretory cathepsin S-like protease of Spirometra mansoni plerocercoid. Parasitology 109 Pt 5 (1994) 611-621
17. Guiliano D.B., Hong X., McKerrow J.H., et al. A gene family of cathepsin L-like proteases of filarial nematodes are associated with larval molting and cuticle and eggshell remodeling. Mol Biochem Parasitol 136 (2004) 227-242
18. Lustigman S., McKerrow J.H., Shah K., et al. Cloning of a cysteine protease required for the molting of Onchocerca volvulus third stage larvae. J Biol Chem 271 (1996) 30181-30189
19. Tort J., Brindley P.J., Knox D., et al. Proteinases and associated genes of parasitic helminths. Adv Parasitol 43 (1999) 161-266
20. Uparanukraw P., Morakote N., Harnnoi T., et al. Molecular cloning of a gene encoding matrix metalloproteinase-like protein from Gnathostoma spinigerum. Parasitol Res 87 (2001) 751-757
21. Sajid M., and McKerrow J.H. Cysteine proteases of parasitic organisms. Mol Biochem Parasitol 120 (2002) 1-21
22. Maleewong W., Intapan P.M., Khempila J., et al. Gnathostoma spinigerum: growth and development of third-stage larvae in vitro. J Parasitol 81 (1995) 800-803
23. Sambrook J., Fritsch E.F., and Maniatis T. Molecular cloning: a laboratory manual. 2nd ed. (1989), Cold Spring Harbor Laboratory Press, Plainview
24. Sakanari J.A., Staunton C.E., Eakin A.E., et al. Serine proteases from nematode and protozoan parasites: isolation of sequence homologs using generic molecular probes. Proc Natl Acad Sci USA 86 (1989) 4863-4867
25. Altschul S.F., Madden T.L., Schaffer A.A., et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucl Acids Res 25 (1997) 3389-3402
26. Bendtsen J.D., Nielsen H., von H.G., et al. Improved prediction of signal peptides: SignalP 3.0. J Mol Biol 340 (2004) 783-795
27. Thompson J.D., Higgins D.G., and Gibson T.J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22 (1994) 4673-4680
28. Tamura K., Dudley J., Nei M., et al. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24 (2007) 1596-1599
29. Bjellqvist B., Hughes G.J., Pasquali C., et al. The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences. Electrophoresis 14 (1993) 1023-1031
30. Choe Y., Leonetti F., Greenbaum D.C., et al. Substrate profiling of cysteine proteases using a combinatorial peptide library identifies functionally unique specificities. J Biol Chem 281 (2006) 12824-12832
31. Harris J.L., Backes B.J., Leonetti F., et al. Rapid and general profiling of protease specificity by using combinatorial fluorogenic substrate libraries. Proc Natl Acad Sci USA 97 (2000) 7754-7759
32. Ron D., and Dressler H. pGSTag-a versatile bacterial expression plasmid for enzymatic labeling of recombinant proteins. Biotechniques 13 (1992) 866-869
33. Morakote N., Nateewatana N., Maleewong W., et al. Anatomical localization of Gnathostoma spinigerum larval antigens by an indirect fluorescent antibody test. Southeast Asian J Trop Med Public Health 20 (1989) 291-295
34. Karrer K.M., Peiffer S.L., and DiTomas M.E. Two distinct gene subfamilies within the family of cysteine protease genes. Proc Natl Acad Sci USA 90 (1993) 3063-3067
35. Vernet T., Berti P.J., de M.C., et al. Processing of the papain precursor. The ionization state of a conserved amino acid motif within the Pro region participates in the regulation of intramolecular processing. J Biol Chem 270 (1995) 10838-10846
36. Britton C., and Murray L. A cathepsin L protease essential for Caenorhabditis elegans embryogenesis is functionally conserved in parasitic nematodes. Mol Biochem Parasitol 122 (2002) 21-33
37. Kirschke H., and Barrett A.J. Cathepsin L-a lysosomal cysteine proteinase. Prog Clin Biol Res 180 (1985) 61-69
38. Tao K., Stearns N.A., Dong J., et al. The proregion of cathepsin L is required for proper folding, stability, and ER exit. Arch Biochem Biophys 311 (1994) 19-27
39. Taylor M.A., Baker K.C., Briggs G.S., et al. Recombinant pro-regions from papain and papaya proteinase IV are selective high affinity inhibitors of the mature papaya enzymes. Protein Eng 8 (1995) 59-62
40. Hellberg A., Nowak N., Leippe M., et al. Recombinant expression and purification of an enzymatically active cysteine proteinase of the protozoan parasite Entamoeba histolytica. Protein Expr Purif 24 (2002) 131-137
41. Barrett A.J., Kirschke H., and Cathepsin B. Cathepsin H, and cathepsin L. Methods Enzymol 80 Pt C (1981) 535-561
42. Smith S.M., and Gottesman M.M. Activity and deletion analysis of recombinant human cathepsin L expressed in Escherichia coli. J Biol Chem 264 (1989) 20487-20495
43. Yamasaki H., Mineki R., Murayama K., et al. Characterisation and expression of the Fasciola gigantica cathepsin L gene. Int J Parasitol 32 (2002) 1031-1042
44. Mathieu M.A., Bogyo M., Caffrey C.R., et al. Substrate specificity of schistosome versus human legumain determined by P1-P3 peptide libraries. Mol Biochem Parasitol 121 (2002) 99-105
45. Hashmi S., Britton C., Liu J., et al. Cathepsin L is essential for embryogenesis and development of Caenorhabditis elegans. J Biol Chem 277 (2002) 3477-3486
46. Brady C.P., Dowd A.J., Brindley P.J., et al. Recombinant expression and localization of Schistosoma mansoni cathepsin L1 support its role in the degradation of host hemoglobin. Infect Immun 67 (1999) 368-374
47. Dahl E.L., and Rosenthal P.J. Biosynthesis, localization, and processing of falcipain cysteine proteases of Plasmodium falciparum. Mol Biochem Parasitol 139 (2005) 205-212