Recombinant expression and purification of an enzymatically active cysteine proteinase of the protozoan parasite Entamoeba histolytica

Protein Expression and Purification

Volumn 24, Issue 1, 2002, Pages 131-137

  Hellberg, A ^a   Nowak, N ^a   Leippe, M ^b   Tannich, E ^a   Bruchhaus, I ^a  

^a BERNHARD NOCHT INSTITUTE FOR TROPICAL MEDICINE   (Germany)

^b UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CYSTEINE PROTEINASE; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; ENTAMOEBA HISTOLYTICA; ENZYME ACTIVATION; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PROTEIN FOLDING;

ANIMALS; CLONING, MOLECULAR; CYSTEINE ENDOPEPTIDASES; ENTAMOEBA HISTOLYTICA; ENZYME ACTIVATION; ESCHERICHIA COLI; GENE EXPRESSION; PROTEIN FOLDING; RECOMBINANT PROTEINS;

BOVINAE; ENTAMOEBA; ENTAMOEBA HISTOLYTICA; ESCHERICHIA COLI; PROTOZOA;

EID: 0035996748     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1548     Document Type: Article

References (27)

1. Role of the Entamoeba histolytica cysteine proteinase in amebic liver abscess formation in severe combined immunodeficient mice
2. Interaction of laminin with Entamoeba histolytica cysteine proteinases and its effect on amebic pathogenesis
3. Antisense inhibition of expression of cysteine proteinases affects Entamoeba histolytica-induced formation of liver abscess in hamsters
4. Entamoeba histolytica cysteine proteinases with interleukin-1 beta converting enzyme (ICE) activity cause intestinal inflammation and tissue damage in amoebiasis
5. Entamoeba histolytica and Entamoeba dispar: Differences in numbers and expression of cysteine proteinase genes
6. Entamoeba histolytica: A novel cysteine protease and an adhesin form the 112 kDa surface protein
7. Affinity purification and biochemical characterization of histolysin, the major cysteine proteinase of Entamoeba histolytica
8. Cysteine endopeptidases of Entamoeba histolytica
9. On the specificity of a cysteine proteinase from Entamoeba histolytica
10. Isolation and molecular characterization of a surface-bound proteinase of Entamoeba histolytica
11. A DNA sequence corresponding to the gene encoding cysteine proteinase 5 in Entamoeba histolytica is present and positionally conserved but highly degenerated in Entamoeba dispar
12. pJC20 and pJC40 - Two high-copy-number vectors for T7 RNA polymerase-dependent expression of recombinant genes in Escherichia coli
13. Cysteine endopeptidases of Entamoeba histolytica
14. Spontaneous release of cysteine proteinases but not of pore-forming peptides by viable Entamoeba histolytica
15. The neutral cysteine proteinase of Entamoeba histolytica degrades IgG and prevents its binding
16. Cysteine protease inhibitors as chemotherapy: Lessons from a parasite target
17. Leishmania major: Molecular modeling of cysteine proteases and prediction of new nonpeptide inhibitors
18. Cysteine protease inhibitors cure an experimental Trypanosoma cruzi infection
19. Expression and subcellular localization of cpn60 protein family members in Leishmania donovani
20. Use of T7 RNA polymerase to direct expression of cloned genes
21. The expression in Escherichia coli of a synthetic gene coding for the precursor of papain is prevented by its own putative signal sequence
22. The sequence, organization, and expression of the major cysteine protease (cruzain) from Trypanosoma cruzi
23. Expression and characterization of a recombinant cysteine proteinase of Leishmania mexicana
24. Active papain renatured and processed from insoluble recombinant propapain expressed in Escherichia coli
25. Action of the major protease from Entamoeba histolytica on proteins of the extracellular matrix
26. Two major ’higher molecular mass’ proteinases of Entamoeba histolytica are identified as cysteine proteinases 1 and 2