Optimal conditions for freezing cho-s and HEK293-EBNA cell lines: Influence of ME2SO, freeze density, and pei-mediated transfection on devitalization and growth of cells, and expression of recombinant protein

Biotechnology and Bioengineering

Volumn 100, Issue 5, 2008, Pages 911-922

  Kleman, Marika I ^a   Oellers, Kerstin ^a   Lullau, Elke ^a  

^a ASTRAZENECA R AND D   (Sweden)

Author keywords

Bulk freezing; Cryopreservation; Mammalian cell; Transient transfection

Indexed keywords

CELL CULTURE; CELLS; DESIGN OF EXPERIMENTS; FREEZING; MAMMALS; RECOMBINANT PROTEINS; THAWING;

BULK FREEZING; CRYO-PRESERVATION; LARGE SCALE BIOREACTORS; MAMMALIAN CELLS; OPTIMAL CONDITIONS; POLYETHYLENEIMINE; PRODUCTION RELIABILITY; TRANSIENT TRANSFECTION;

MOLECULAR BIOLOGY;

POLYETHYLENEIMINE; RECOMBINANT PROTEIN; CRYOPROTECTIVE AGENT; DIMETHYL SULFOXIDE;

ANIMAL CELL; ARTICLE; CELL GROWTH; CELL STRAIN HEK293; CELL VIABILITY; CENTRIFUGATION; CHO CELL; CRYOPRESERVATION; CULTURE MEDIUM; EMBRYO; EXPERIMENTAL STUDY; FREEZING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; NONHUMAN; PLASMID; PROTEIN EXPRESSION; ANIMAL; BIOSYNTHESIS; CELL LINE; CELL PROLIFERATION; CELL SURVIVAL; CHEMISTRY; CRICETULUS; CULTURE TECHNIQUE; CYTOLOGY; DRUG EFFECT; HAMSTER; KIDNEY; METABOLISM; METHODOLOGY; PROTEIN ENGINEERING;

MAMMALIA;

ANIMALS; CELL CULTURE TECHNIQUES; CELL LINE; CELL PROLIFERATION; CELL SURVIVAL; CHO CELLS; CRICETINAE; CRICETULUS; CRYOPRESERVATION; CRYOPROTECTIVE AGENTS; DIMETHYL SULFOXIDE; FREEZING; HUMANS; KIDNEY; POLYETHYLENEIMINE; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 48449091840     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21832     Document Type: Article

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