A new seed-train expansion method for recombinant mammalian cell lines

Cytotechnology

Volumn 38, Issue 1-3, 2002, Pages 99-108

  Heidemann, Rüdiger ^a   Mered, Mokhtar ^a   Wang, D Q ^a   Gardner, Bruce ^a   Zhang, Chun ^a   Michaels, James ^a   Henzler, Hans Jürgen ^b   Abbas, Nada ^a   Konstantinov, Konstantin ^a  

^a BAYER CORPORATION   (United States)

^b BAYER AG   (Germany)

Author keywords

Cell banking; Cell culture; Cryo bags; Inoculation bioreactor; One step inoculation; Seed train expansion

Indexed keywords

MAMMALIA; VISCUM; VISCUM ALBUM;

EID: 0036417849     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1021114300958     Document Type: Conference Paper

References (20)

1. Avis KE & Wagner CM (1999) Cryopreservation - Applications in pharmaceuticals and biotechnology. Drug Manufacturing Technology Series Volume 5: Interpharm Press, Denver, Colorado.
2. Chuppa S, Tsai Y-S, Yoon S, Shackleford S, Rozales C, Bhat R, Tsay G, Matanguihan C, Konstantinov K & Naveh D (1997) Fermentor temperature as a tool for control of high-density perfusion cultures of mammalian cells. Biotechnol Bioeng 55: 328-338.
3. Diener B, Utesch D, Beer N, Dürk H & Oesch F (1993) A method for the cryopreservation of liver parenchymal cells for studies of xenobiotics. Cryobiology 30: 116-127.
4. Fennema OR, Powrie WD & Marth EH (1973) Low-temperature Preservation of Food and Living Matter. Marcel Dekker, Inc., New York.
5. Franks F (1985) Biophysics and Biochemistry at Low Temperatures. Cambridge University Press.
6. Franks F (1991) Freeze-drying: From empiricism to predictability: The significance of glass transitions. Karger, Basel. Dev Biol Stand 74: 9-19.
7. Grout B, Morris J & McLellan M (1990) Cryopreservation and the maintenance of cell lines. Trends Biotechnol 8: 293-297.
8. Heidemann R, Zhang C, Qi H, Rule J, Rozales C, Park S, Chuppa S, Ray M, Michaels J, Konstantinov K & Naveh D (2000) The use of peptones as medium additives for the production of a recombinant therapeutic protein in high density perfusion cultures of mammalian cells. Cytotechnology 32: 157-167.
9. Heldman DR & Singh RP (1981) Food Process Engineering, 2nd Ed., The AVI Publishing Company, Inc., Westport, Connecticut, pp. 178-184.
10. Lindl T & Bauer J (1989) Zell- und Gewebekultur. 2. Aufl. Gustav Fischer Verlag.
11. Morgan SJ & Darling DC (1993) Animal Cell Culture. Bios Scientific Publishers, Oxford.
12. Ninomiya N, Shirahata S, Murakami H & Sugahara T (1991) Large-scale, high density freezing of hybridomas and its application to high-density culture. Biotechnol Bioeng 38: 1110-1113.
13. Re A, Vijayaraghhavan K, Basade M, He S & Gulati S (1998) Long-term cryopreservation: Successful trilineage engraftment after autologous bone marrow transplantation with bone marrow cryopreserved for seven years, J Hematotherapy 7: 185-188.
14. Regidor C, Posada M, Monteagudo D, Garauled C, Somolinos N, Forés R, Briz M & Fernández M (1999) Stem cell transplantation. Exp Hematology 27: 380-385.
15. Siegel WH (1999) Cryopreservation of mammailian cell cultures. In Avis KE and Wagner CM (eds) Cryopreservation - Applications in Pharmaceuticals and Biotechnology. Drug Manufacturing Technology Series Volume 5, Interpharm Press, Denver, Colorado.
16. Thrift J, Tsai Y, Lowe B, Ng M, Michaels J, Hey J & Konstantinov, (2000) Development and optimization of protein-free cell banking technology. Proceedings of the Cell Culture Engineering VII Foundation Meeting, Santa Fee, NM, 5-10 February.
17. Whitaker SC, Francis R & Siegel RC (1998) Validation of continuously perfused cell culture processes for production of monoclonal antibodies. In Kelly BD and Ramelmeier A (eds) Validation of Biopharmaceutical Manufacturing Processes. American Chemical Society, pp. 28-43.
18. Wu L, Sun J, Wang L, Woodman K, Koutalistras N, Horvat M & Sheil AGR (2000) Cryopreservation of primary porcine hepatocytes for use in bioartificial liver support systems. Transplantation Proc 32: 2271-2272.
19. Wu L, Sun J, Wang L, Woodman K, Koutalistras N, Horvat M & Sheil AGR (2001) Cryopreserved porcine hepatocytes in a liver biodialysis system. Transplantation Proc 33: 1950-1951.
20. Zachariou M, Mazer J, Park H & Olson C (2001) Method for Evaluating Target Protein Quality from Fermenter. US Patent 6,214,975 B1.