Kinetics of IFN-γ producing CHO cells and other industrially relevant cell lines in rapeseed-supplemented batch cultures

Process Biochemistry

Volumn 43, Issue 9, 2008, Pages 945-953

  Farges, B ^a   Chenu, S ^b   Marc, A ^a   Goergen, J L ^a  

^a UNIVERSITÉ DE LORRAINE   (France)

^b INSTITUT DE RECHERCHE PIERRE FABRE   (France)

Author keywords

Amino acid supplementation; Bioreactor cultures; CHO cells; Rapeseed peptides; Recombinant proteins; Serum free medium

Indexed keywords

AMINES; AMINO ACIDS; AMMONIA; ANIMAL CELL CULTURE; BATCH CELL CULTURE; BATCH DATA PROCESSING; BIOCHEMISTRY; CELL GROWTH; CELLS; CHEMOTHERAPY; ECOLOGY; GLUCOSE; GROWTH KINETICS; OCCUPATIONAL RISKS; RISK ASSESSMENT; WELL STIMULATION;

BATCH CULTURES; BATCH PROCESSING; CELL DENSITIES; CELL LINES; CHINESE HAMSTER OVARY (CHO) CELLS; CULTURE MEDIA (CM); CULTURE MEDIUM; DERIVED PRODUCTS; ELSEVIER (CO); IN ORDER; LACTATE (LAC); PRODUCTION PROCESSES; RISK OF CONTAMINATION; SERUM FREE CONDITIONS; SPECIFIC PRODUCTION RATE;

CELL CULTURE;

ANIMALIA; BACTERIA (MICROORGANISMS); BRASSICA NAPUS;

EID: 47949132766     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2008.04.025     Document Type: Article

References (64)

1. Chu L., and Robinson D.K. Industrial choices for protein production by large-scale cell culture. Curr Opin Biotechnol 12 (2001) 180-187
2. Werner R.G. Economic aspects of commercial manufacture of biopharmaceuticals. J Biotechnol 113 (2004) 171-182
3. Froud S.J. The development, benefits and disadvantages of serum-free media. Dev Biol Stand 99 (1999) 157-166
4. Van der Valk J., Mellor D., Brands R., Fischer R., Gruber F., Gstraunthaler G., et al. The humane collection of fetal bovine serum and possibilities for serum-free cell and tissue culture. Toxicol In Vitro 18 (2004) 1-12
5. Keen M.J., and Rapson N.T. Development of a serum-free culture medium for the large scale production of recombinant protein from Chinese hamster ovary cell line. Cytotechnology 17 (1995) 153-163
6. Castro P.M., Hayter P.M., Ison A.P., and Bull A.T. Application of a statistical design to the optimization of culture medium for recombinant interferon-gamma production by Chinese hamster ovary cells. Appl Microbiol Biotechnol 38 (1992) 84-90
7. Stoll T.S., Muhlethaler K., Von Stockar U., and Marison I.W. Systematic improvement of a chemically-defined protein-free medium for hybridoma growth and monoclonal antibody production. J Biotechnol 45 (1996) 111-123
8. Lee G.M., Kim E.J., Kim N.S., Yoon S.K., Ahn Y.H., and Song J.Y. Development of a serum-free medium for the production of erythropoietin by suspension culture of recombinant Chinese hamster ovary cells using a statistical design. J Biotechnol 69 (1999) 85-93
9. Dyring C., Hansen H.A., and Emborg C. Observations on the influence of glutamine, asparagine and peptone on growth and t-PA production of Chinese hamster ovary (CHO) cells. Cytotechnology 16 (1994) 37-42
10. Jan D.C., Jones S.J., Emery A.N., and Al-Rubeai M. Peptone, a low-cost growth-promoting nutrient for intensive animal cell culture. Cytotechnology 16 1 (1994) 17-26
11. Merten O.W., Kierulff J.V., Castignolles N., and Perrin P. Evaluation of the new serum-free medium (MDSS2) for the production of different biologicals: use of various cell lines. Cytotechnology 14 (1994) 47-59
12. Perrin P., Madhusudana S., Gontier-Jallet C., Petres S., Tordo N., and Merten O.W. An experimental rabies vaccine produced with a new BHK-21 suspension cell culture process: use of serum-free medium and perfusion-reactor system. Vaccine 13 13 (1995) 1244-1250
13. Donaldson M.S., and Shuler M.L. Low-cost serum-free medium for the BTI-Tn5B1-4 insect cell line. Biotechnol Prog 14 (1998) 573-579
14. Nyberg G.B., Balcarcel R.R., Follstad B.D., Stephanopoulos G., and Wang D.I.C. Metabolism of peptide amino acids by Chinese Hamster Ovary cells grown in a complex medium. Biotechnol Bioeng 62 (1999) 324-335
15. Schlaeger E.J. The protein hydrolysate, Primatone RL, is a cost-effective multiple growth promoter of mammalian cell culture in serum-containing and serum-free media and displays anti-apoptosis properties. J Immunol Methods 194 (1996) 191-199
16. Asher D.M. Bovine sera used in the manufacture of biologicals: current concerns and policies of the U.S. Food and Drug Administration regarding the transmissible spongiform encephalopathies. Dev Biol Stand 99 (1999) 41-44
17. Franek F., Hohenwarter O., and Katinger H. Plant protein hydrolysates: preparation of defined peptide fractions promoting growth and production in animal cells cultures. Biotechnol Prog 16 (2000) 688-692
18. Heidemann R., Zhang C., Qi H., Rule J.L., Rozales C., Park S., et al. The use of peptones as medium additives for the production of a recombinant therapeutic protein in high-density perfusion cultures of mammalian cells. Cytotechnology 32 (2000) 157-167
19. Burteau C., Verhoeye F., Mols J., Ballez J.S., Agathos S., and Schneider Y.J. Fortification of a protein-free cell culture medium with plant peptones improves cultivation and productivity of an interferon-gamma-producing CHO cell line. In Vitro Cell Dev Biol: Anim 39 (2003) 291-296
20. Deparis V., Durrieu C., Schweizer M., Goergen J.L., Chevalot I., and Marc A. Promoting effect of rapeseed proteins and peptides on Sf9 insect cell growth. Cytotechnology 42 (2003) 75-85
21. Ballez J.S., Mols J., Burteau C., Agathos S.N., and Schneider Y.J. Plant protein hydrolysates support CHO-320 cells proliferation and recombinant IFN-gamma production in suspension and inside microcarriers in protein-free media. Cytotechnology 44 (2004) 103-114
22. Clare D.A., and Swaisgood H.E. Bioactive milk peptides: a prospectus. J Dairy Sci 83 (2000) 1187-1195
23. Smacchi E., and Gobetti M. Bioactive peptides in dairy products: synthesis and interaction with proteolytic enzymes. Food Microbiol 17 (2000) 129-141
24. Mols J., Peeters-Joris C., Wattiez R., Agathos S.N., and Schneider Y.J. Recombinant interferon-gamma secreted by CHO-320 cells cultivated in suspension in protein-free media is protected against extracellular proteolysis by the expression of natural protease inhibitors and by the addition of plant protein hydrolysates to the culture medium. In Vitro Cell Dev Biol: Anim 41 (2005) 83-91
25. Minamoto Y., Ogawa K., Hideki A., Iochi Y., and Misuki K. Development of a serum-free and heat sterilizable medium and continuous high-density cell culture. Cytotechnology 5 (1991) S35-S51
26. Franek F., and Katinger H. Specific effects of synthetic oligopeptides on cultured animal cells. Biotechnol Prog 18 1 (2002) 155-158
27. Godon B. Protéines végétales. Les méthodes courantes de laboratoire pour la séparation et l'analyse des protéines végétales. In: Godon B. (Ed). Protéines végétales (1996), Lavoisier TecDoc, Paris 65-80
28. Farges-Haddani B., Tessier B., Chenu S., Harscoat C., Marc I., Goergen J.L., et al. Peptide fractions of rapeseed hydrolysates as an alternative to animal proteins in CHO cell culture media. Process Biochem 41 (2006) 2297-2304
29. Tessier B., Schweizer M., Fournier F., Framboisier X., Chevalot I., Vanderesse R., et al. Prediction of the amino acid composition of small peptides contained in a plant protein hydrolysate by LC-MS and CE-MS. Food Res Int 38 (2005) 577-584
30. Vioque J., Sánchez-Vioque R., Clemente A., Pedroche J., and Millán F. Partially hydrolyzed rapeseed protein isolates with improved functional properties. J Am Oil Chem Soc 77 (2000) 447-450
31. Kozlowska H., Naczk M., Shahidi F., and Zadernowski R. Phenolic acids and tannins in rapeseed and canola. In: Shahidi F. (Ed). Canola and rapeseed. Production, chemistry, nutrition and processing technology (1990), Van Nostrand Reinhold, USA 193-210
32. Lin Y.C., Wu C.N., Shih S.R., and Ho M.S. Characterization of a Vero cell-adapted virulent strain of enterovirus 71 suitable for use as a vaccine candidate. Vaccine 20 (2002) 2485-2493
33. Kuzuhara S., Nakamura H., Hayashida K., Obata J., Abe M., Sonoda K., et al. Non-clinical and phase I clinical trials of a Vero cell-derived inactivated Japanese encephalitis vaccine. Vaccine 21 (2003) 4519-4526
34. Whitford W. NS0 serum-free culture and applications. BioProcess Int (December 2003) 36-47
35. Mols J. Cultivation of Chinese hamster ovary (CHO) cells in protein-free media supplemented with plant peptones is sufficient to prevent proteolysis of secreted recombinant glycoproteins. PhD thesis. Université catholique de Louvain; 2004.
36. Monaco L., Tagliabue R., Soria M.R., and Uhlèn M. An in vitro approach for the expression of recombinant proteins in mammalian cells. Biotechnol Appl Biochem 20 (1994) 157-171
37. Monaco L., Marc A., Eon-Duval A., Acerbis G., Distefano G., Lamotte D., et al. Genetic engineering of α2,6-sialyltransferase in recombinant CHO cells and its effect on the sialylation of recombinant interferon-γ. Cytotechnology 22 (1996) 197-203
38. Lamotte D., Buckberry L., Monaco L., Soria M., Jenkins N., Engasser J.M., et al. Na-butyrate increases the production and α2,6-sialylation of recombinant interferon-γ expressed by α2,6-sialyltransferease engineered CHO cells. Cytotechnology 29 (1999) 55-64
39. Naczk M., Shahidi F., and Sullivan A. Recovery of rapeseed tannins by various solvent systems. Food Chem 45 1 (1992) 51-54
40. Brinkmann M., Lütkemeyer D., Gudermann F., and Lehmann J. New technologies for automated cell counting based on optical image analysis "The Cellscreen". Cytotechnology 38 (2002) 119-127
41. Nielsen L.K., Smyth G.K., and Greenfield P.F. Hemacytometer cell count distributions: implications of non-Poisson behaviour. Biotechnol Prog 7 (1991) 560-563
42. Renard J.M., Spagnoli R., Mazier C., Salles M.F., and Mandine E. Evidence that monoclonal antibody production kinetics is related to the integral of viable cells in batch systems. Biotechnol Lett 10 (1988) 91-96
43. Sanfeliu A., Cairo J.J., Casas C., Sola C., and Godia F. Analysis of nutritional factors and physical conditions affecting growth and monoclonal antibody production of the hybridoma KB-26.5 cell line. Biotechnol Prog 12 (1996) 209-216
44. Franek F. Peptides modulate growth and productivity of mammalian cell cultures and suppress apoptosis. BioProcess Int (June 2004) 48-52
45. Franek F., Eckschlager T., and Katinger H. Enhancement of monoclonal antibody production by lysine-containing peptides. Biotechnol Prog 19 (2003) 169-174
46. Schlaeger E.J., and Schumpp B. Studies of mammalian cell growth in suspension culture. In: Spier R.E., Griffiths J.B., and Heigmier B. (Eds). Advances in animal cell biology and technology for bioprocesses (1989), Sevenoaks, Kent 386-396
47. Ozturk S.S., Riley M.R., and Palsson B.O. Effects of ammonia and lactate on hybridoma growth, metabolism, and antibody-production. Biotechnol Bioeng 39 (1992) 418-431
48. Martinelle K., and Haggstrom L. Mechanisms of ammonia and ammonium ion toxicity in animal cells: transport across cell membranes. J Biotechnol 30 (1993) 339-350
49. Newland M., Kamal M.N., Greenfield P.F., and Nielsen L.K. Ammonia inhibition of hybridomas propagated in batch, fed-batch, and continuous culture. Biotechnol Bioeng 43 (1994) 434-438
50. Lao M.S., and Toth D. Effects of ammonium and lactate on growth and metabolism of a recombinant Chinese hamster ovary cell culture. Biotechnol Prog 13 (1997) 688-691
51. Geaugey V., Duval D., Geahel I., Marc A., and Engasser J.M. Influence of amino acids on hybridoma cell viability and antibody secretion. Cytotechnology 2 (1989) 119-129
52. Bollati Fogolín M., Wagner R., Etcheverrigaray M., and Kratje R. Impact of temperature reduction and expression of yeast pyruvate carboxylase on hGM-CSF-producing CHO cells. J Biotechnol 109 (2004) 179-191
53. Bonarius H.P.J., Hatzimanikatis V., Meesters K.P.H., de Gooijer C.D., Schmid G., and Tramper J. Metabolic flux analysis of hybridoma cells in different culture media using mass balances. Biotechnol Bioeng 50 (1996) 299-318
54. Sung Y.H., Lim S.W., Chung J.Y., and Lee G.M. Yeast hydrolysate as a low-cost additive to serum-free medium for the production of human thrombopoietin in suspension cultures of Chinese hamster ovary cells. Appl Microbiol Biotechnol 63 (2004) 527-536
55. Ganapathy V., and Leibach F. Role of pH gradient and membrane potential in dipeptide transport in intestinal and renal brush-border membrane vesicles from the rabbit. Studies with l-carnosine and glycyl-l-proline. J Biol Chem 258 (1983) 14189-14192
56. Ganapathy V., Burckhardt G., and Leibach F.H. Characteristics of glycylsarcosine transport in rabbit intestinal brush-border membrane vesicles. J Biol Chem 259 (1984) 8954-8959
57. Takuwa N., Shimada T., Matsumoto H., Himukai M., and Hoshi T. Effect of hydrogen ion-gradient on carrier-mediated transport of glycylglycine across brush border membrane vesicles from rabbit small intestine. Jpn J Physiol 35 (1985) 629-642
58. Daniel H., Morse E.L., and Adibi S.A. The high and low affinity transport systems for dipeptides in kidney brush border membrane respond differently to alterations in pH gradient and membrane potential. J Biol Chem 266 (1991) 19917-19924
59. Kastner A., Frochot C., Dumas D., Vanderesse R., and Marc A. Confocal microscopy observation of CHO cells cultivated in presence of fluorescent-labelled rapeseed peptides. In: Noll T. (Ed). Proceedings of the 20th ESACT meeting on cells and culture. Dresden, Germany, June 17-20 (2007)
60. Nakayama T., Niimi T., Osawa T., and Kawakishi S. The protective role of polyphenols in cytotoxicity of hydrogen peroxide. Mutat Res 281 (1992) 77-80
61. Naczk M., Amarowicz R., Sullivan A., and Shahidi F. Current research developments on polyphenolics of rapeseed/canola: a review. Food Chem 62 (1998) 489-502
62. Terada S., Nishimura T., Sasaki M., Yamada H., and Miki M. Sericin a protein derived from silkworms, accelerates the proliferation of several mammalian cell lines including a hybridoma. Cytotechnology 40 (2002) 3-12
63. Zhang J., and Robinson D. Development of animal-free, protein-free and chemically-defined media for NS0 cell culture. Cytotechnology 48 (2005) 59-74
64. Chabanon G., Alves da Costa L., Farges B., Harscoat C., Chenu S., Goergen J.-L., Marc A., Marc I., and Chevalot I. Influence of the rapeseed protein hydrolysis process on CHO cell growth. Bioresource Technol 99 (2008) 7143-7151