Bothrops protease A, a unique highly glycosylated serine proteinase, is a potent, specific fibrinogenolytic agent

Journal of Thrombosis and Haemostasis

Volumn 6, Issue 8, 2008, Pages 1363-1372

  Paes Leme, A F ^a   Prezoto, B C ^a   Yamashiro, E T ^a   Bertholim, L ^a   Tashima, A K ^a   Klitzke, C F ^a   Camargo, A C M ^a   Serrano, Solange M T ^a  

^a INSTITUTO BUTANTAN   (Brazil)

Author keywords

Bothrops; BPA; Fibrinogenolytic activity; Glycosylation; Serine proteinase

Indexed keywords

ALBUMIN; ANTITHROMBIN III; APROTININ; BENZAMIDINE; BOTHROPS PROTEASE A; FIBRIN ANTIBODY; FIBRINOLYTIC AGENT; FIBRONECTIN; HEPARIN; N DEGLYCOSYLATED BOTHROPS PROTEASE A; SERINE PROTEINASE; SERINE PROTEINASE INHIBITOR; VITRONECTIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTROLLED STUDY; DRUG MECHANISM; FIBRINOGEN BLOOD LEVEL; FIBRINOGENOLYSIS; HUMAN; IN VITRO STUDY; IN VIVO STUDY; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN GLYCOSYLATION; RAT; STRUCTURE ACTIVITY RELATION; THROMBOGENESIS; THROMBUS; TWO DIMENSIONAL ELECTROPHORESIS; WESTERN BLOTTING;

ANIMALS; FIBRINOGEN; FIBRINOLYTIC AGENTS; GLYCOSYLATION; HUMANS; MALE; RATS; RATS, WISTAR; SERINE ENDOPEPTIDASES; SERINE PROTEINASE INHIBITORS; SNAKE VENOMS; SUBSTRATE SPECIFICITY; THROMBOSIS;

EID: 47649123459     PISSN: 15387933     EISSN: 15387836     Source Type: Journal    
DOI: 10.1111/j.1538-7836.2008.02995.x     Document Type: Article

References (37)

1. Barrett AJ, Rawlings ND. Families and clans of serine peptidases. Arch Biochem Biophys 1995; 318: 247-50.
2. Serrano SM, Maroun RC. Snake venom serine proteinases: Sequence homology vs. substrate specificity, a paradox to be solved. Toxicon 2005; 45: 1115-32.
3. Mandelbaum FR, Henriques OB. Purification and properties of Bothrops protease A. Arch Biochem Biophys 1964; 104: 369-74.
4. Murayama N, Saguchi K, Mentele R, Assakura MT, Ohi H, Fujita Y, Camargo AC, Higuchi S, Serrano SM. The unusual high molecular mass of Bothrops protease A, a trypsin-like serine peptidase from the venom of Bothrops jararaca, is due to its high carbohydrate content. Biochim Biophys Acta 2003; 1652: 1-6.
5. Kini RM. Anticoagulant proteins from snake venoms: Structure, function and mechanism. Biochem J 2006; 397: 377-87.
6. Jakobsen E, Kierulf P. A modified b-alanine precipitation procedure to prepare fibrinogen free of antithrombin-III and plasminogen. Thromb Res 1973; 3: 145-59.
7. Willis TW, Tu AT. Purification and biochemical characterization of atroxase, a nonhemorrhagic fibrinolytic protease from western diamondback rattlesnake venom. Biochemistry 1988; 27: 4769-77.
8. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-5.
9. Hanna SL, Sherman NE, Kinter MT, Goldberg JB. Comparison of proteins expressed by Pseudomonas aeruginosa strains representing initial and chronic isolates from a cystic fibrosis patient: An analysis by 2-D gel electrophoresis and capillary column liquid chromatography-tandem mass spectrometry. Microbiology 2000; 146: 2495-508.
10. Steel LF, Trotter MG, Nakajima PB, Mattu TS, Gonye G, Block T. Efficient and specific removal of albumin from human serum samples. Mol Cell Proteomics 2003; 2: 262-70.
11. Burnette WN. 'Western blotting': Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem 1981; 112: 195-203.
12. Serrano SM, Shannon JD, Wang D, Camargo AC, Fox JW. A multifaceted analysis of viperid snake venoms by two-dimensional gel electrophoresis: an approach to understanding venom proteomics. Proteomics 2005; 5: 501-10.
13. Giles AR. Guidelines for the use of animals in biomedical research. Thromb Haemost 1987; 58: 1078-84.
14. Reyers I, Mussoni L, Donati BL, Gaetano G. Failure of aspirin at different doses to modify experimental thrombosis in rats. Thromb Res 1980; 18: 669-74.
15. Maffei FHA, Rollo HA, Fabris VE. Prevention of experimental venous thrombosis induced by contrast medium in the rat. Acta Radiol Diagn 1980; 21: 249-52.
16. Grannis GF. Plasma fibrinogen: Determination, normal values, physiopathologic shifts, and fluctuations. Clin Chem 1970; 16: 486-94.
17. Grannis GF, Kazal LA, Tocantins LM. The spectrophotometric determination of thrombin activity in plasma. Thromb Diath Haemorrh 1965; 13: 330-42.
18. Fiedler F, Geiger R, Hirschauer C, Leysath G. Peptide esters and nitroanilides as substrates for the assay of human urinary kallikrein. Hoppe-Seyler's Z Physiol Chem 1978; 259: 1667-73.
19. Mandelbaum FR, Carrillo M, Henriques SB. Proteolytic activity of Bothrops protease A on the B chain of oxidized insulin. Biochim Biophys Acta 1967; 132: 508-10.
20. Schechter I, Berger A. On the size of the active site in proteases. I. Papain. Biochem Biophys Res Commun 1967; 27: 157-62.
21. Nishida S, Fujimura Y, Miura S, Ozaki Y, Usami Y, Suzuki M, Titani K, Yoshida E, Sugimoto M, Yoshioka A, Fukuit H. Purification and characterization of bothrombin, a fibrinogen-clotting serine protease from the venom of Bothrops jararaca. Biochemistry 1994; 33: 1843-9.
22. Serrano SMT, Mentele R, Sampaio CAM, Fink E. Purification, characterization, and amino acid sequence of a serine proteinase, PABJ, with platelet-aggregating activity from the venom of Bothrops jararaca. Biochemistry 1995; 34: 7186-93.
23. Serrano SMT, Hagiwara Y, Murayama N, Higuchi S, Mentele R, Sampaio CAM, Camargo ACM, Fink E. Purification and characterization of a kinin-releasing and fibrinogen-clotting serine proteinase proteinase (KN-BJ) from the venom of Bothrops jararaca, and molecular cloning and sequence analysis of its cDNA. Eur J Biochem 1998; 251: 845-53.
24. Serrano SMT, Sampaio CAM, Mentele R, Camargo ACM, Fink E. A novel fibrinogen-clotting enzyme, TL-BJ, from the venom of the snake Bothrops jararaca: Purification and characterization. Thromb Haemost 2000; 83: 438-44.
25. Swenson S, Markland FS Jr. Snake venom fibrin(ogen)olytic enzymes. Toxicon 2005; 45: 1021-39.
26. Lee JW, Seu JH, Rhee IK, Jin I, Kawamura Y, Park W. Purification and characterization of brevinase, a heterogeneous two-chain fibrinolytic enzyme from the venom of Korean snake, Agkistrodon blomhoffii brevicaudus. Biochem Biophys Res Commun 1999; 260: 665-70.
27. Guo YW, Chang TY, Lin KT, Liu HW, Shih KC, Cheng SH. Cloning and functional expression of the mucrosobin protein, a beta-fibrinogenase of Trimeresurus mucrosquamatus (Taiwan Habu). Protein Expr Purif 2001; 23: 483-90.
28. Hung CC, Chiou SH. Isolation of multiple isoforms of alpha-fibrinogenase from the Western diamondback rattlesnake, Crotalus atrox: N-terminal sequence homology with ancrod, an antithrombotic agent from Malayan viper. Biochem Biophys Res Commun 1994; 201: 1414-23.
29. Hung CC, Chiou SH. Expression of a kallikrein-like protease from the snake venom: Engineering of autocatalytic site in the fusion protein to facilitate protein refolding. Biochem Biophys Res Commun 2000; 275: 924-30.
30. He J, Chen S, Gu J. Identification and characterization of Harobin, a novel fibrino(geno)lytic serine protease from a sea snake (Lapemis hardwickii). FEBS Lett 2007; 581: 2965-73.
31. Murakami MT, Arni RK. Thrombomodulin-independent activation of protein C and specificity of hemostatically active snake venom serine proteinases: crystal structures of native and inhibited Agkistrodon contortrix contortrix protein C activator. J Biol Chem 2005; 280: 39309-15.
32. Zhu Z, Liang Z, Zhang T, Zhu Z, Xu W, Teng M, Niu L. Crystal structures and amidolytic activities of two glycosylated snake venom serine proteinases. J Biol Chem 2005; 280: 10524-9.
33. Page MJ, Macgillivray RT, Di Cera E. Determinants of specificity in coagulation proteases. J Thromb Haemost 2005; 3: 2401-8.
34. Liu M, Counsell C, Zhao XL, Wardlaw J. Fibrinogen depleting agents for acute ischaemic stroke. Cochrane Database Syst Rev 2003; 3: CD000091.
35. Rötker J, Preissner KT, Muller-Berghaus G. Soluble fibrin consists of fibrin oligomers of heterogeneous distribution. Eur J Biochem 1986; 155: 583-8.
36. Dempfle C-E, Argiriou S, Kucher K, Müller-Peltzer H, Rübsamen K, Heene DL. Hemostasis, thrombosis, and vascular biology. Analysis of fibrin formation and proteolysis during intravenous administration of ancrod. Blood 2000; 96: 2793-802.
37. Fox JW, Serrano SM. Approaching the golden age of natural product pharmaceuticals from venom libraries: An overview of toxins and toxin-derivatives currently involved in therapeutic or diagnostic applications. Curr Pharm Des 2007; 13: 2927-34.