Alternative splicing and exon duplication generates 10 unique porcine 5-HT4 receptor splice variants including a functional homofusion variant

Physiological Genomics

Volumn 34, Issue 1, 2008, Pages 22-33

  De Maeyer, Joris H ^a,b,c,d   Aerssens, Jeroen ^b,e   Verhasselt, Peter ^b   Lefebvre, Romain A ^a  

^a GHENT UNIVERSITY   (Belgium)

^b JANSSEN RESEARCH AND DEVELOPMENT   (Belgium)

^c Movetis NV   (Belgium)

^d Movetis   (Belgium)

^e TIBOTEC BVBA   (Belgium)

Author keywords

5 hydroxytryptamine; 7 transmembrane receptor; 9 transmembrane receptor; G protein coupled receptor; Pig

Indexed keywords

DIMER; G PROTEIN COUPLED RECEPTOR; SEROTONIN 4 RECEPTOR; SEROTONIN 4B2 RECEPTOR; SEROTONIN 4J RECEPTOR; SEROTONIN 4K RECEPTOR; SEROTONIN 4L RECEPTOR; SEROTONIN 4M RECEPTOR; SEROTONIN 4O RECEPTOR; SEROTONIN 4P RECEPTOR; SEROTONIN 4Q RECEPTOR; SEROTONIN 4R RECEPTOR; UNCLASSIFIED DRUG; BENZOFURAN DERIVATIVE; CYCLIC AMP; ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; ISOPROTEIN; PRUCALOPRIDE; SEROTONIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; ARTIFACT; CARBOXY TERMINAL SEQUENCE; EXON; FEMALE; GENE DUPLICATION; GENE FUSION; GENETIC VARIABILITY; GENOME; HTR4 GENE; HUMAN; HUMAN CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PIGLET; PRIORITY JOURNAL; PROTEIN DOMAIN; RECEPTOR GENE; RNA SPLICING; ALTERNATIVE RNA SPLICING; AMINO ACID SEQUENCE; ANIMAL; CELL LINE; CELL MEMBRANE; CHEMISTRY; DRUG EFFECT; DRUG POTENTIATION; GENE EXPRESSION PROFILING; GENETIC TRANSFECTION; GENETICS; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; SEQUENCE ALIGNMENT; SWINE;

ANIMALIA; SUIDAE; SUS;

ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; ANIMALS; BENZOFURANS; CELL LINE; CELL MEMBRANE; CLONING, MOLECULAR; CYCLIC AMP; EXONS; FEMALE; GENE EXPRESSION PROFILING; GENOME; GREEN FLUORESCENT PROTEINS; HUMANS; KINETICS; MOLECULAR SEQUENCE DATA; PROTEIN ISOFORMS; RECEPTORS, SEROTONIN, 5-HT4; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SEROTONIN; SWINE; TRANSFECTION;

EID: 47249085680     PISSN: 10948341     EISSN: 15312267     Source Type: Journal    
DOI: 10.1152/physiolgenomics.00038.2008     Document Type: Article

References (42)

1. Arai M, Mitsuke H, Ikeda M, Xia JX, Kikuchi T, Satake M, Shimizu T. ConPred II: a consensus prediction method for obtaining transmembrane topology models with high reliability. Nucleic Acids Res 32: W390-W393, 2004.
2. Armengol L, Pujana MA, Cheung J, Scherer SW, Estivill X. Enrichment of segmental duplications in regions of breaks of synteny between the human and mouse genomes suggest their involvement in evolutionary rearrangements. Hum Mol Genet 12: 2201-2208, 2003.
3. 4 splice variant. J Neurochem 74: 478-489, 2000.
4. Bhave G, Nadin BM, Brasier DJ, Glauner KS, Shah RD, Heinemann SF, Karim F, Gereau RWT. Membrane topology of a metabotropic glutamate receptor. J Biol Chem 278: 30294-30301, 2003.
5. Bockaert J, Claeysen S, Becamel C, Dumuis A, Marin P. Neuronal 5-HT metabotropic receptors: fine-tuning of their structure, signaling, and roles in synaptic modulation. Cell Tissue Res 326: 553-572, 2006.
6. 4 receptors. Curr Drug Targets CNS Neurol Disord 3: 39-51, 2004.
7. 4(i). Naunyn Schmiedebergs Arch Pharmacol 369: 616-628, 2004.
8. Claeysen S, Sebben M, Becamel C, Bockaert J, Dumuis A. Novel brain-specific 5-HT4 receptor splice variants show marked constitutive activity: role of the C-terminal intracellular domain. Mol Pharmacol 55: 910-920, 1999.
9. Claros MG, von Heijne G. TopPred II: an improved software for membrane protein structure predictions. Comput Appl Biosci 10: 685-686, 1994.
10. Dumuis A, Bouhelal R, Sebben M, Cory R, Bockaert J. A nonclassical 5-hydroxytryptamine receptor positively coupled with adenylate cyclase in the central nervous system. Mol Pharmacol 34: 880-887, 1988.
11. Freeman JL, Perry GH, Feuk L, Redon R, McCarroll SA, Altshuler DM, Aburatani H, Jones KW, Tyler-Smith C, Hurles ME, Carter NP, Scherer SW, Lee C. Copy number variation: new insights in genome diversity. Genome Res 16: 949-961, 2006.
12. Gabriel D, Vernier M, Pfeifer MJ, Dasen B, Tenaillon L, Bouhelal R. High throughput screening technologies for direct cyclic AMP measurement. Assay Drug Dev Technol 1: 291-303, 2003.
13. 4 receptor: molecular cloning and pharmacological characterization of two splice variants. EMBO J 14: 2806-2815, 1995.
14. Grider JR, Foxx-Orenstein AE, Jin JG. 5-Hydroxytryptamine4 receptor agonists initiate the peristaltic reflex in human, rat, and guinea pig intestine. Gastroenterology 115: 370-380, 1998.
15. 4a receptor splice variant: roles in receptor targeting. J Cell Sci 117: 5367-5379, 2004.
16. 4 receptor and its consequences in early BALB/c mouse embryonic development. BMC Dev Biol 7: 34, 2007.
17. 4-like receptors. Naunyn Schmiedebergs Arch Pharmacol 342: 619-622, 1990.
18. Kaumann AJ, Levy FO. Fading of 5-HT4 receptor-mediated inotropic responses to 5-hydroxytryptamine is caused by phosphodiesterase activity in porcine atrium. Br J Pharmacol 147: 128-130, 2006.
19. Kemp A, Manahan-Vaughan D. The 5-hydroxytryptamine4 receptor exhibits frequency-dependent properties in synaptic plasticity and behavioural metaplasticity in the hippocampal CA1 region in vivo. Cereb Cortex 15: 1037-1043, 2005.
20. Kilpatrick GJ, Dautzenberg FM, Martin GR, Eglen RM. 7TM receptors: the splicing on the cake. Trends Pharmacol Sci 20: 294-301, 1999.
21. Krogh A, Larsson B, von Heijne G, Sonnhammer EL. Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol 305: 567-580, 2001.
22. Lahbib-Mansais Y, Mompart F, Milan D, Leroux S, Faraut T, Delcros C, Yerle M. Evolutionary breakpoints through a high-resolution comparative map between porcine chromosomes 2 and 16 and human chromosomes. Genomics 88: 504-512, 2006.
23. 4 receptor ligands: applications and new prospects. J Med Chem 46: 319-344, 2003.
24. Linardopoulou E, Mefford HC, Nguyen O, Friedman C, van den Engh G, Farwell DG, Coltrera M, Trask BJ. Transcriptional activity of multiple copies of a subtelomerically located olfactory receptor gene that is polymorphic in number and location. Hum Mol Genet 10: 2373-2383, 2001.
25. 4 receptors in the mouse enteric nervous system. Am J Physiol Gastrointest Liver Physiol 289: G1148-G1163, 2005.
26. Manuel-Apolinar L, Rocha L, Pascoe D, Castillo E, Castillo C, Meneses A. Modifications of 5-HT4 receptor expression in rat brain during memory consolidation. Brain Res 1042: 73-81, 2005.
27. 4 receptors partial agonist, and an antagonist. Neuropharmacology 47: 1021-1035, 2004.
28. 4 receptor in the central nervous system by TaqMan real time RT-PCR. Brain Res Mol Brain Res 90: 125-134, 2001.
29. 4(d) receptor desensitization in CHO cells. Br J Pharmacol 138: 445-452, 2003.
30. Moller S, Croning MD, Apweiler R. Evaluation of methods for the prediction of membrane spanning regions. Bioinformatics 17: 646-653, 2001.
31. Niesler B, Frank B, Kapeller J, Rappold GA. Cloning, physical mapping and expression analysis of the human 5-HT3 serotonin receptor-like genes HTR3C, HTR3D and HTR3E. Gene 310: 101-111, 2003.
32. 4b receptors is splice variant dependent. Biosci Rep 24: 215-223, 2004.
33. 4 receptor splice variants: compound specificity and dual coupling with Galphas- and Galphai/o-proteins. Mol Pharmacol 61: 85-96, 2002.
34. Ponimaskin EG, Heine M, Joubert L, Sebben M, Bickmeyer U, Richter DW, Dumuis A. The 5-hydroxytryptamine(4a) receptor is palmitoylated at two different sites, and acylation is critically involved in regulation of receptor constitutive activity. J Biol Chem 277: 2534-2546, 2002.
35. Ponimaskin EG, Schmidt MF, Heine M, Bickmeyer U, Richter DW. 5-Hydroxytryptamine 4(a) receptor expressed in Sf9 cells is palmitoylated in an agonist-dependent manner. Biochem J 353: 627-634, 2001.
36. 4 receptor-mediated inotropic response to serotonin in heart failure. Cardiovasc Res 65: 869-878, 2005.
37. 4 receptor isoforms on beta-amyloid secretion. Neurodegener Dis 5: 163-165, 2008.
38. Schworer H, Ramadori G. Autoreceptors can modulate 5-hydroxytryptamine release from porcine and human small intestine in vitro. Naunyn Schmiedebergs Arch Pharmacol 357: 548-552, 1998.
39. Shimogiri T, Kiuchi S, Hiraiwa H, Hayashi T, Takano Y, Maeda Y, Yasue H. Assignment of 117 genes from HSA5 to the porcine IMpRH map and generation of a dense human-pig comparative map. Anim Genet 37: 503-508, 2006.
40. Ullmer C, Schmuck K, Kalkman HO, Lubbert H. Expression of serotonin receptor mRNAs in blood vessels. FEBS Lett 370: 215-221, 1995.
41. Vogel C, Morea V. Duplication, divergence and formation of novel protein topologies. Bioessays 28: 973-978, 2006.
42. Zhang L, Lu HH, Chung WY, Yang J, Li WH. Patterns of segmental duplication in the human genome. Mol Biol Evol 22: 135-141, 2005.