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Volumn 47, Issue 12, 2008, Pages 5473-5484

Tuning the activity of Zn(II) complexes in DNA cleavage: Clues for design of new efficient metallo-hydrolases

Author keywords

[No Author keywords available]

Indexed keywords

HYDROLASE; ZINC;

EID: 47049106872     PISSN: 00201669     EISSN: None     Source Type: Journal    
DOI: 10.1021/ic800085n     Document Type: Article
Times cited : (54)

References (133)
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    • The products of BNPP hydrolysis were identified by means of 1H and 31P NMR measurements The 1H NMR spectrum recorded after mixing BNPP and the Zn(II) complex at pH 9 shows the appearance of the signals of p-nitrophenate, NP (two doublets at 6.72 and 8.28 ppm, and of mono(p-nitrophenyl) phosphate, MNPP (two doublets at 7.29 and 8.20 ppm, Integration of the signals accounts for a 1:1 molar ratio between the NP and MNPP formed in the hydrolytic process. At the same time, the signal of MNPP (a singlet at 0.6 ppm) appears in the 31P spectrum. The time evolution of the 31P spectra shows a progressive increase of intensity of this signal and a simultaneous decrease of the BNPP signal. No other signal, including that of inorganic phosphate at 3.79 ppm, was observed in 31PNMR spectra, even for prolonged reaction times, indicating that the MNPP is not further hydrolyzed by the Zn(II) complexes
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* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.