A chromatin immunoprecipitation screen in mouse keratinocytes reveals Runx1 as a direct transcriptional target of ΔNp63

Journal of Cellular Biochemistry

Volumn 104, Issue 4, 2008, Pages 1204-1219

  Ortt, Kori ^a   Raveh, Eli ^b   Gat, Uri ^b   Sinha, Satrajit ^a,c  

^a UNIVERSITY AT BUFFALO   (United States)

^b HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^c 121 Farber Hall ^*  (United States)

Author keywords

Chromatin immunoprecipitation; Hair follicle; Keratinocyte; p63; Runx1; Transcription

Indexed keywords

GENE PRODUCT; PROTEIN DELTANP63; PROTEIN P63; PROTEIN TAP63; SMALL INTERFERING RNA; TRANSCRIPTION FACTOR RUNX1;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL MATURATION; CELL PROLIFERATION; CELL TRANSFORMATION; CHROMATIN IMMUNOPRECIPITATION; CONTROLLED STUDY; DNA DETERMINATION; ENHANCER REGION; EPIDERMIS; GENE; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENOMICS; HAIR FOLLICLE; HUMAN; KERATINOCYTE; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; P63 GENE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RUNX1 GENE; TRANSCRIPTION REGULATION;

ANIMALS; CHROMATIN IMMUNOPRECIPITATION; CORE BINDING FACTOR ALPHA 2 SUBUNIT; GENE EXPRESSION REGULATION; HAIR FOLLICLE; KERATINOCYTES; MICE; PHOSPHOPROTEINS; RESPONSE ELEMENTS; TRANS-ACTIVATORS; TRANSCRIPTION, GENETIC;

ANIMALIA; MUS;

EID: 46949086501     PISSN: 07302312     EISSN: 10974644     Source Type: Journal    
DOI: 10.1002/jcb.21700     Document Type: Article

References (40)

1. Antonini D, Rossi B, Han R, Minichiello A, Di Palma T, Corrado M, Banfi S, Zannini M, Brissette JL, Missero C. 2006. An autoregulatory loop directs the tissue-specific expression of p63 through a long-range evolutionarily conserved enhancer. Mol Cell Biol 26:3308-3318.
2. Barbieri CE, Pietenpol JA. 2006. p63 and epithelial biology. Exp Cell Res 312:695-706.
3. Barbieri CE, Tang LJ, Brown KA, Pietenpol JA. 2006. Loss of p63 leads to increased cell migration and up-regulation of genes involved in invasion and metastasis. Cancer Res 66:7589-7597.
4. Birkaya B, Ortt K, Sinha S. 2007. Novel in vivo targets of DeltaNp63 in keratinocytes identified by a modified chromatin immunoprecipitation approach. BMC Mol Biol 8:43.
5. Blanpain C, Fuchs E. 2007. p63: Revving up epithelial stem-cell potential. Nat Cell Biol 9:731-733.
6. Candi E, Rufini A, Terrinoni A, Dinsdale D, Ranalli M, Paradisi A, De Laurenzi V, Spagnoli LG, Catani MV, Ramadan S, Knight RA, Melino G. 2006. Differential roles of p63 isoforms in epidermal development: Selective genetic complementation in p63 null mice. Cell Death Differ 13:1037-1047.
7. Carroll DK, Carroll JS, Leong CO, Cheng F, Brown M, Mills AA, Brugge JS, Ellisen LW. 2006. p63 regulates an adhesion programme and cell survival in epithelial cells. Nat Cell Biol 8:551-561.
8. Ghozi MC, Bernstein Y, Negreanu V, Levanon D, Groner Y. 1996. Expression of the human acute myeloid leukemia gene AML1 is regulated by two promoter regions. Proc Natl Acad Sci USA 93:1935-1940.
9. Kaufman CK, Zhou P, Pasolli HA, Rendl M, Bolotin D, Lim KC, Dai X, Alegre ML, Fuchs E. 2003. GATA-3: An unexpected regulator of cell lineage determination in skin. Genes Dev 17:2108-2122.
10. Kim TH, Ren B. 2006. Genome-wide analysis of protein-DNA interactions. Annu Rev Genomics Hum Genet 7:81-102.
11. King KE, Weinberg WC. 2007. p63: Defining roles in morphogenesis, homeostasis, and neoplasia of the epidermis. Mol Carcinog 46:716-724.
12. Koster MI, Roop DR. 2004. The role of p63 in development and differentiation of the epidermis. J Dermatol Sci 34:3-9.
13. Koster MI, Kim S, Mills AA, DeMayo FJ, Roop DR. 2004. p63 is the molecular switch for initiation of an epithelial stratification program. Genes Dev 18:126-131.
14. Koster MI, Dai D, Marinari B, Sano Y, Costanzo A, Karin M, Roop DR. 2007. p63 induces key target genes required for epidermal morphogenesis. Proc Natl Acad Sci USA 104:3255-3260.
15. Kramer I, Sigrist M, de Nooij JC, Taniuchi I, Jessell TM, Arber S. 2006. A role for Runx transcription factor signaling in dorsal root ganglion sensory neuron diversification. Neuron 49:379-393.
16. Levanon D, Groner Y. 2004. Structure and regulated expression of mammalian RUNX genes. Oncogene 23: 4211-4219.
17. Marmigere F, Montelius A, Wegner M, Groner Y, Reichardt LF, Ernfors P. 2006. The Runx1/AML1 transcription factor selectively regulates development and survival of TrkA nociceptive sensory neurons. Nat Neurosci 9:180-187.
18. Mikhail FM, Sinha KK, Saunthararajah Y, Nucifora G. 2006. Normal and transforming functions of RUN X1:A perspective. J Cell Physiol 207:582-593.
19. Mikkola ML. 2007. p63 in skin appendage development. Cell Cycle 6:285-290.
20. Mills AA, Zheng B, Wang XJ, Vogel H, Roop DR, Bradley A. 1999. p63 is a p53 homologue required for limb and epidermal morphogenesis. Nature 398:708-713.
21. Naoe Y, Setoguchi R, Akiyama K, Muroi S, Kuroda M, Hatam F, Littman DR, Taniuchi I. 2007. Repression of interleukin-4 in T helper type 1 cells by Runx/Cbf beta binding to the 114 silencer. J Exp Med 204:1749-1755.
22. Odom DT, Dowell RD, Jacobsen ES, Gordon W, Danford TW, MacIsaac KD, Rolfe PA, Conboy CM, Gifford DK, Fraenkel E. 2007. Tissue-specific transcriptional regulation has diverged significantly between human and mouse. Nat Genet 39:730-732.
23. Ortt K, Sinha S. 2006. Derivation of the consensus DNA-binding sequence for p63 reveals unique requirements that are distinct from p53. FEBS Lett 580:4544-4550.
24. Osada M, Park HL, Nagakawa Y, Yamashita K, Fomenkov A, Kim MS, Wu B, Nomoto S, Trink B, Sidranksy D. 2005. Differential recognition of response elements determines target gene specificity for p53 and p63. Mol Cell Biol 25:6077-6089.
25. Perez CA, Ott J, Mays DJ, Pietenpol JA. 2007. p63 consensus DNA-binding site: Identification, analysis and application into a p63MH algorithm. Oncogene.
26. Raveh E, Cohen S, Levanon D, Negreanu V, Groner Y, Gat U. 2006. Dynamic expression of Runx1 in skin affects hair structure. Mech Dev 123:842-850.
27. Romano RA, Birkaya B, Sinha S. 2006. Defining the regulatory elements in the proximal promoter of DeltaNp63 in keratinocytes: Potential roles for Sp1/Sp3, NF-Y, and p63. J Invest Dermatol 126:1469-1479.
28. Romano RA, Birkaya B, Sinha S. 2007. A functional enhancer of keratin 14 is a direct transcriptional target of deltaNp63. J Invest Dermatol 127:1175-1186.
29. Suh EK, Yang A, Kettenbach A, Bamberger C, Michaelis AH, Zhu Z, Elvin JA, Bronson RT, Crum CP, McKeon F. 2006. p63 protects the female germ line during meiotic arrest. Nature 444:624-628.
30. Truong AB, Kretz M, Ridky TW, Kimmel R, Khavari PA. 2006. p63 regulates proliferation and differentiation of developmentally mature keratinocytes. Genes Dev 20:3185-3197.
31. van Bokhoven H, McKeon F. 2002. Mutations in the p53 homolog p63: Allele-specific developmental syndromes in humans. Trends Mol Med 8:133-139.
32. Vidal VP, Chaboissier MC, Lutzkendorf S, Cotsarelis G, Mill P, Hui CC, Ortonne N, Ortonne JP, Schedl A. 2005. Sox9 is essential for outer root sheath differentiation and the formation of the hair stem cell compartment. Curr Biol 15:1340-1351.
33. Vigano MA, Mantovani R. 2007. Hitting the numbers: The emerging network of p63 targets. Cell Cycle 6:233-239.
34. Vigano MA, Lamartine J, Testoni B, Merico D, Alotto D, Castagnoli C, Robert A, Candi E, Melino G, Gidrol X, Mantovani R. 2006. New p63 targets in keratinocytes identified by a genome-wide approach. EMBO J 25:5105-5116.
35. Wang X, Blagden C, Fan J, Nowak SJ, Taniuchi I, Littman DR, Burden SJ. 2005. Runx1 prevents wasting, myofibrillar disorganization, and autophagy of skeletal muscle. Genes Dev 19:1715-1722.
36. Yang A, Kaghad M, Wang Y, Gillett E, Fleming MD, Dotsch V, Andrews NC, Caput D, McKeon F. 1998. p63, a p53 homolog at 3q27-29, encodes multiple products with transactivating, death-inducing, and dominant-negative activities. Mol Cell 2:305-316.
37. Yang A, Schweitzer R, Sun D, Kaghad M, Walker N, Bronson RT, Tabin C, Sharpe A, Caput D, Crum C, McKeon F. 1999. p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development. Nature 398:714-718.
38. Yang A, Kaghad M, Caput D, McKeon F. 2002. On the shoulders of giants: p63, p73 and the rise of p53. Trends Genet 18:90-95.
39. Yang A, Zhu Z, Kapranov P, McKeon F, Church GM, Gingeras TR, Struhl K. 2006. Relationships between p63 binding, DNA sequence, transcription activity, and biological function in human cells. Mol Cell 24:593-602.
40. Zhou G, Zheng Q, Engin F, Munivez E, Chen Y, Sebald E, Krakow D, Lee B. 2006. Dominance of SOX9 function over RUNX2 during skeletogenesis. Proc Natl Acad Sci USA 103:19004-19009.