The NMR structure and dynamics of the two-domain tick carboxypeptidase inhibitor reveal flexibility in its free form and stiffness upon binding to human carboxypeptidase B

Biochemistry

Volumn 47, Issue 27, 2008, Pages 7066-7078

  Pantoja Uceda, David ^a   Arolas, Joan L ^b   García, Pascal ^c,d   López Hernández, Eva ^c   Padró, Daniel ^c,e   Aviles, Francesc X ^b   Blanco, Francisco J ^f  

^a INSTITUTO DE QUÍMICA FÍSICA ROCASOLANO   (Spain)

^b UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^c Centro Nacional de Investigations Oncológicas   (Spain)

^d Oncura   (Spain)

^e CIC BIOMAGUNE   (Spain)

^f CIC BIOGUNE   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

AMIDES; CHEMOTHERAPY; CONFORMATIONS; CRYSTAL STRUCTURE; DYNAMICS; FLOW INTERACTIONS; MECHANISMS; NUCLEAR MAGNETIC RESONANCE; PORT TERMINALS; STABILIZATION;

ACTIVE SITES; AMERICAN CHEMICAL SOCIETY (ACS); AMIDE PROTONS; BACKBONE DYNAMICS; BIO-MEDICAL APPLICATIONS; C TERMINUS; C-TERMINAL DOMAIN (CTD); CARBOXYPEPTIDASE; COMPLEX FORMATIONS; COMPLEX STABILIZATION; FREE FORM; GLOBULAR DOMAINS; INDUCED FIT; N TERMINAL DOMAIN (NTD); NMR SPECTROS COPY; RECOGNITION MECHANISM; SOLVENT EXCHANGING; SPECIFIC INHIBITORS; STRUCTURAL DIFFERENCES; STRUCTURE AND DYNAMICS; STRUCTURE-FUNCTION RELATIONSHIPS; TERMINAL RESIDUES; TWO DOMAINS;

NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

AMIDE; CARBOXYPEPTIDASE; CARBOXYPEPTIDASE A; CARBOXYPEPTIDASE B; ENZYME INHIBITOR; METALLOCARBOXYPEPTIDASE; SOLVENT; TICK CARBOXYPEPTIDASE INHIBITOR;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; CONFORMATION; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INHIBITOR INTERACTION; ENZYME REGULATION; FIBRINOLYTIC THERAPY; HUMAN; MOLECULAR RECOGNITION; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN STRUCTURE; STRUCTURE ACTIVITY RELATION;

AMIDES; AMINO ACID SEQUENCE; ANIMALS; CARBOXYPEPTIDASE B; ENZYME INHIBITORS; HUMANS; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PLIABILITY; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PROTONS; SOLUTIONS; THERMODYNAMICS; TICKS;

IXODIDA;

EID: 46849104270     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi800403m     Document Type: Article

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