Lineage selection of functional and cryopreservable human embryonic stem cell-derived neurons

Stem Cells

Volumn 26, Issue 7, 2008, Pages 1705-1712

  Ladewig, Julia ^a   Koch, Philipp ^a   Endl, Elmar ^a   Meiners, Banu ^a   Opitz, Thoralf ^a   Couillard Despres, Sebastien ^b   Aigner, Ludwig ^b   Brüstle, Oliver ^a  

^a UNIVERSITY OF BONN   (Germany)

^b UNIVERSITY OF REGENSBURG   (Germany)

Author keywords

Cryopreservation; Human embryonic stem cells; Human neurons; Lineage selection

Indexed keywords

CASPASE; DOUBLECORTIN; GREEN FLUORESCENT PROTEIN;

ARTICLE; CELL DIFFERENTIATION; CELL LINEAGE; CELL MATURATION; CELL SELECTION; CONTROLLED STUDY; CRYOPRESERVATION; EMBRYONIC STEM CELL; ENZYME INHIBITION; FLUORESCENCE ACTIVATED CELL SORTER; HUMAN; HUMAN CELL; NERVE CELL; PROTEIN EXPRESSION;

ANIMALS; CELL LINEAGE; CELLS, CULTURED; CRYOPRESERVATION; ELECTROPHYSIOLOGY; EMBRYONIC STEM CELLS; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; HIPPOCAMPUS; HUMANS; MICE; MICROTUBULE-ASSOCIATED PROTEINS; NEURONS; NEUROPEPTIDES; PROMOTER REGIONS (GENETICS);

EID: 46749124012     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1634/stemcells.2008-0007     Document Type: Article

References (40)

1. Reubinoff BE, Itsykson P, Turetsky T et al. Neural progenitors from human embryonic stem cells. Nat Biotechnol 2001;19:1134-1140.
2. Zhang SC, Wernig M, Duncan ID et al. In vitro differentiation of transplantable neural precursors from human embryonic stem cells. Nat Biotechnol 2001;19:1129-1133.
3. Yoshizaki T, Inaji M, Kouike H et al. Isolation and transplantation of dopaminergic neurons generated from mouse embryonic stem cells. Neurosci Lett 2004;363:33-37.
4. Brederlau A, Correia AS, Anisimov SV et al. Transplantation of human embryonic stem cell-derived cells to a rat model of Parkinson's disease: Effect of in vitro differentiation on graft survival and teratoma formation. STEM CELLS 2006;24:1433-1440.
5. Bjorklund A, Lindvall O. Cell replacement therapies for central nervous system disorders. Nat Neurosci 2000;3:537-544.
6. Koch P, Siemen H, Biegler A et al. Transduction of human embryonic stem cells by ecotropic retroviral vectors. Nucleic Acids Res 2006;34:e120.
7. Koch P, Driehaus J, Opitz T et al. Neural stem cells from human ES cells. Program 615.4/A4. 2006 Abstract Viewer and Itinerary Planner. Atlanta, GA: Society for Neuroscience, 2006. Online.
8. Couillard-Despres S, Winner B, Schaubeck S et al. Doublecortin expression levels in adult brain reflect neurogenesis. Eur J Neurosci 2005;21:1-14.
9. Polleux F, Ghosh A. The slice overlay assay: A versatile tool to study the influence of extracellular signals on neuronal development. Sci STKE 2002;136:pl9.
10. Scheffler B, Schmandt T, Schroder W et al. Functional network integration of embryonic stem cell-derived astrocytes in hippocampal slice cultures. Development 2003;130:5533-5541.
11. Opitz T, Scheffler B, Steinfarz B et al. Electrophysiological evaluation of engrafted stem cell-derived neurons. Nat Protoc 2007;2:1603-1613.
12. Gleeson JG, Lin PT, Flanagan LA et al. Doublecortin is a microtubule-associated protein and is expressed widely by migrating neurons. Neuron 1999;23:257-271.
13. Francis F, Koulakoff A, Boucher D et al. Doublecortin is a developmentally regulated, microtubule-associated protein expressed in migrating and differentiating neurons. Neuron 1999;23:247-256.
14. Conti L, Pollard SM, Gorba T et al. Niche-independent symmetrical self-renewal of a mammalian tissue stem cell. PLoS Biol 2005;3:e283.
15. Jain M, Armstrong RJ, Tyers P et al. GABAergic immunoreactivity is predominant in neurons derived from expanded human neural precursor cells in vitro. Exp Neurol 2003;182:113-123.
16. Zhang SC. Neural subtype specification from embryonic stem cells. Brain Pathol 2006;16:132-142.
17. Benninger F, Beck H, Wernig M et al. Functional integration of embryonic stem cell-derived neurons in hippocampal slice cultures. J Neurosci 2003;23:7075-7083.
18. Heng BC, Ye CP, Liu H et al. Loss of viability during freeze-thaw of intact and adherent human embryonic stem cells with conventional slow-cooling protocols is predominantly due to apoptosis rather than cellular necrosis. J Biomed Sci 2006;13:433-445.
19. Stroh C, Cassens U, Samraj AK et al. The role of caspases in cryoinjury: Caspase inhibition strongly improves the recovery of cryopreserved hematopoietic and other cells. FASEB J 2002;16:1651-1653.
20. Heng BC, Clement MV, Cao T. Caspase inhibitor Z-VAD-FMK enhances the freeze-thaw survival rate of human embryonic stem cells. Biosci Rep 2007;27:257-264.
21. Jungling K, Nagler K, Pfrieger FW et al. Purification of embryonic stem cell-derived neurons by immunoisolation. FASEB J 2003;17:2100-2102.
22. Schmandt T, Meents E, Gossrau G et al. High-purity lineage selection of embryonic stem cell-derived neurons. Stem Cells Dev 2005;14:55-64.
23. Windrem MS, Nunes MC, Rashbaum WK et al. Fetal and adult human oligodendrocyte progenitor cell isolates myelinate the congenitally dysmyelinated brain. Nat Med 2004;10:93-97.
24. Pruszak J, Sonntag KC, Aung MH et al. Markers and methods for cell sorting of human embryonic stem cell-derived neural cell populations. STEM CELLS 2007;25:2257-2268.
25. Seldeslagh KA, Lauweryns JM. NCAM expression in the pulmonary neural and diffuse neuroendocrine cell system. Microsc Res Tech 1997;37:69 -76.
26. Real FX, Houghton AN, Albino AP et al. Surface antigens of melanomas and melanocytes defined by mouse monoclonal antibodies: Specificity analysis and comparison of antigen expression in cultured cells and tissues. Cancer Res 1985;45:4401-4411.
27. Kobari L, Giarratana MC, Pflumio F et al. CD133+ cell selection is an alternative to CD34+ cell selection for ex vivo expansion of hematopoietic stem cells. J Hematother Stem Cell Res 2001;10:273-281.
28. Wernig M, Tucker KL, Gornik V et al. Tau EGFP embryonic stem cells: An efficient tool for neuronal lineage selection and transplantation. J Neurosci Res 2002;69:918-924.
29. Thompson LH, Andersson E, Jensen JB et al. Neurogenin2 identifies a transplantable dopamine neuron precursor in the developing ventral mesencephalon. Exp Neurol 2006;198:183-198.
30. Wichterle H, Lieberam I, Porter JA et al. Directed differentiation of embryonic stem cells into motor neurons. Cell 2002;110:385-397.
31. Rao MS, Shetty AK. Efficacy of doublecortin as a marker to analyse the absolute number and dendritic growth of newly generated neurons in the adult dentate gyrus. Eur J Neurosci 2004;19:234-246.
32. Wang X, Qiu R, Tsark W et al. Rapid promoter analysis in developing mouse brain and genetic labeling of young neurons by doublecortin-DsRed-express. J Neurosci Res 2007;85:3567-3573.
33. Couillard-Despres S, Winner B, Karl C et al. Targeted transgene expression in neuronal precursors: Watching young neurons in the old brain. Eur J Neurosci 2006;24:1535-1545.
34. Yancey PH. Organic osmolytes as compatible, metabolic and counter-acting cytoprotectants in high osmolarity and other stresses. J Exp Biol 2005;208:2819-2830.
35. Asada M, Ishibashi S, Ikumi S et al. Effect of polyvinyl alcohol (PVA) concentration during vitrification of in vitro matured bovine oocytes. Theriogenology 2002;58:1199-1208.
36. Alessandri B, Nishioka T, Heimann A et al. Caspase-dependent cell death involved in brain damage after acute subdural hematoma in rats. Brain Res 2006;1111:196-202.
37. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006;126:663-676.
38. Takahashi K, Tanabe K, Ohnuki M et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 2007;131:861-872.
39. Yu J, Vodyanik MA, Smuga-Otto K et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 2007;318:1917-1920.
40. Park IH, Zhao R, West JA et al. Reprogramming of human somatic cells to pluripotency with defined factors. Nature 2008;451:141-146.