Electrophysiological evaluation of engrafted stem cell-derived neurons

Nature Protocols

Volumn 2, Issue 7, 2007, Pages 1603-1613

  Opitz, Thoralf ^a   Scheffler, Björn ^a,b   Steinfarz, Barbara ^a   Schmandt, Tanja ^a   Bröstle, Oliver ^a  

^a UNIVERSITY OF BONN   (Germany)

^b UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; BRAIN SLICE; CELL FUNCTION; CELL INTERACTION; CELL LABELING; CELL POPULATION; CELL STRUCTURE; CELL VIABILITY; CONTROLLED STUDY; DONOR; EFFECTOR CELL; ELECTROPHYSIOLOGY; IMMUNOCYTOCHEMISTRY; INFORMATION PROCESSING; NERVE CELL; NERVE CELL MEMBRANE POTENTIAL; NEURAL STEM CELL; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; PURIFICATION; RAT; SIGNAL TRANSDUCTION; SYNAPSE;

ANIMALS; ELECTROPHYSIOLOGY; FEMALE; HIPPOCAMPUS; MALE; NEURONS; RATS; STEM CELL TRANSPLANTATION; STEM CELLS;

EID: 34447104535     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2007.230     Document Type: Article

References (54)

1. Wichterle, H., Lieberam, I., Porter, J.A. & Jessell, T.M. Directed differentiation of embryonic stem cells into motor neurons. Cell 110, 385-397 (2002).
2. Ahn, J.I. et al. Comprehensive transcriptome analysis of differentiation of embryonic stem cells into midbrain and hindbrain neurons. Dev. Biol. 265, 491-501 (2004).
3. Barberi, T. et al. Neural subtype specification of fertilization and nuclear transfer embryonic stem cells and application in parkinsonian mice. Nat. Biotechnol. 21, 1200-1207 (2003).
4. Watanabe, K. et al. Directed differentiation of telencephalic precursors from embryonic stem cells. Nat. Neurosci. 8, 288-296 (2005).
5. Andersson, E. et al. Identification of intrinsic determinants of midbrain dopamine neurons. Cell 124, 393-405 (2006).
6. Kawasaki, H. et al. Induction of midbrain dopaminergic neurons from ES cells by stromal cell-derived inducing activity. Neuron 28, 31-40 (2000).
7. Lee, S.H. et al. Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cells. Nat Biotechnol. 18, 675-679 (2000).
8. Li, X.J. et al. Specification of motoneurons from human embryonic stem cells. Nat. Biotechnol. 23, 215-221 (2005).
9. Perrier, A.L. et al. Derivation of midbrain dopamine neurons from human embryonic stem cells. Proc. Natl. Acad. Sci. USA 101, 12543-12548 (2004).
10. Zeng, X. et al. Dopaminergic differentiation of human embryonic stem cells. Stem Cells 22, 925-940 (2004).
11. Scheffler, B., Edenhofer, F. & Brustle, O. Merging fields: Stem cells in neurogenesis, transplantation, and disease modeling. Brain Pathol. 16, 155-168 (2006).
12. Goldman, S. Stem and progenitor cell-based therapy of the human central nervous system. Nat. Biotechnol. 23, 862-871 (2005).
13. Gorba, T. & Allsopp, T.E. Pharmacological potential of embryonic stem cells. Pharmacol. Res. 47, 269-278 (2003).
14. Lindvall, O., Kokaia, Z. & Martinez-Serrano, A. Stem cell therapy for human neurodegenerative disorders - how to make it work. Nat. Med. 10 (Suppl.): S42-S50 (2004).
15. Wobus, A.M. & Boheler, K.R. Embryonic stem cells: Prospects for developmental biology and cell therapy. Physiol Rev. 85, 635-678 (2005).
16. Walz, W., Boulton, A.A. & Baker, G.B. in Patch-Clamp Analyis. Advanced Techniques. 1st edn. (ed. Hoather-Potter, K.) 346 (Humana Press, Totowa, NJ, 2002).
17. Sakmann, B. & Neher, E. Single-channel Recording. 2nd edn. (Plenum Press, New York, 1995).
18. Hille, B. Ion Channels of Excitable Membranes. 3rd edn. 814 (Sinauer Associates, Sunderland, MA, 2001).
19. Brüstle, O., Maskos, U. & McKay, R.D.G. Host-guided migration allows targeted introduction of neurons into the embryonic brain. Neuron 15, 1275-1285 (1995).
20. Brüstle, O. et al. In vitro-generated neural precursors participate in mammalian brain development. Proc. Natl. Acad. Sci. USA 94, 14809-14814 (1997).
21. Wernig, M. et al. Functional integration of embryonic stem cell-derived neurons in vivo. J. Neurosci. 24, 5258-5268 (2004).
22. Brüstle, O. et al. Chimeric brains generated by intraventricular transplantation of fetal human brain cells into embryonic rats. Nat. Biotechnol. 16, 1040-1044 (1998).
23. Benninger, F. et al. Functional integration of embryonic stem cell-derived neurons in hippocampal slice cultures. J. Neurosci. 23, 7075-7083 (2003).
24. Scheffler, B. et al. Functional network integration of embryonic stem cell-derived astrocytes in hippocampal slice cultures. Development 130, 5533-5541 (2003).
25. Auerbach, J.M., Eiden, M.V. & McKay, R.D. Transplanted CNS stem cells form functional synapses in vivo. Eur. J. Neurosci. 12, 1696-1704 (2000).
26. Englund, U. et al. Grafted neural stem cells develop into functional pyramidal neurons and integrate into host cortical circuitry. Proc. Natl. Acad. Sci. USA 99, 17089-17094 (2002).
27. Schmandt, T. et al. High-purity lineage selection of embryonic stem cell-derived neurons. Stem Cells Dev. 14, 55-64 (2005).
28. Wernig, M. et al. Tau EGFP embryonic stem cells: An efficient tool for neuronal lineage selection and transplantation. J. Neurosci. Res. 69, 918-924 (2002).
29. Koch, P. et al. Transduction of human embryonic stem cells by ecotropic retroviral vectors. Nucleic Acids Res. 34, e120 (2006).
30. Cesnulevicius, K. et al. Nucleofection is the most efficient nonviral transfection method for neuronal stem cells derived from ventral mesencephali with no changes in cell composition or dopaminergic fate. Stem Cells 24, 2776-2791 (2006).
31. Pusch, M. & Neher, E. Rates of diffusional exchange between small cells and a measuring patch pipette. Pflugers. Arch. 411, 204-211 (1988).
32. Hadjantonakis, A.K. & Nagy, A. The color of mice: In the light of GFP-variant reporters. Histochem. Cell Biol. 115, 49-58 (2001).
33. Duden, R. Live cell imaging: The "green revolution" continues apace. Trends Cell Biol. 12, 548 (2002).
34. van Roessel, P. & Brand, A.H. Imaging into the future: Visualizing gene expression and protein interactions with fluorescent proteins. Nat. Cell Biol. 4, E15-E20 (2002).
35. Miyawaki, A., Sawano, A. & Kogure, T. Lighting up cells: Labelling proteins with fluorophores. Nat. Cell Biol. (Suppl.): S1-S7 (2003).
36. Young, P. & Feng, G. Labeling neurons in vivo for morphological and functional studies. Curr. Opin. Neurobiol. 14, 642-646 (2004).
37. Thomas, N. Fluorescent proteins and engineered cell lines. Methods Mol. Biol. 356, 165-187 (2007).
38. van den Pol, A.N. & Ghosh, P.K. Selective neuronal expression of green fluorescent protein with cytomegalovirus promoter reveals entire neuronal arbor in transgenic mice. J. Neurosci. 18, 10640-10651 (1998).
39. Chung, S. et al. Analysis of different promoter systems for efficient transgene expression in mouse embryonic stem cell lines. Stem Cells 20, 139-145 (2002).
40. Chung, S. & Kim, K.S. Transfection and promoter analysis in embryonic stem cells. Methods Mol. Biol. 329, 187-193 (2006).
41. Zeng, X. et al. Stable expression of hrGFP by mouse embryonic stem cells: Promoter activity in the undifferentiated state and during dopaminergic neural differentiation. Stem Cells 21, 647-653 (2003).
42. Siemen, H. et al. Nucleofection of human embryonic stem cells. Stem Cells Dev. 14, 378-383 (2005).
43. Wallace, H., Ansell, R., Clark, J. & McWhir, J. Pre-selection of integration sites imparts repeatable transgene expression. Nucleic Acids Res. 28, 1455-1464 (2000).
44. Lakshmipathy, U. et al. Efficient transfection of embryonic and adult stem cells. Stem Cells 22, 531-543 (2004).
45. Miyoshi, G. & Fishell, G. Directing neuron-specific transgene expression in the mouse CNS. Curr. Opin. Neurobiol. 16, 577-584 (2006).
46. Terada, N. et al. Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion. Nature 416, 542-545 (2002).
47. Ying, Q.L., Nichols, J., Evans, E.P. & Smith, A.G. Changing potency by spontaneous fusion. Nature 416, 545-548 (2002).
48. Alvarez-Dolado, M. et al. Fusion of bone-marrow-derived cells with Purkinje neurons, cardiomyocytes and hepatocytes. Nature 425 968-973 (2003).
49. Chen, K.A. et al. Fusion of neural stem cells in culture. Exp. Neurol. 198, 129-135 (2006).
50. Yan, J. et al. Extensive neuronal differentiation of human neural stem cell grafts in adult rat spinal cord. PLoS Med. 4, e39 (2007).
51. Goetz, A.K. et al. Temporally restricted substrate interactions direct fate and specification of neural precursors derived from embryonic stem cells. Proc. Natl. Acad. Sci. USA 103, 11063-11068 (2006).
52. Doyle, M.W. et al. Strategies for cellular identification in nucleus tractus solitarius slices. J. Neurosci. Methods 137, 37-48 (2004).
53. Brüstle, O. Building brains: Neural chimeras in the study of nervous system development and repair. Brain Pathol. 9, 527-545 (1999).
54. Brüstle, O., Cunningham, M., Tabar, V. & Studer, L. Experimental transplantation in the embryonic, neonatal, and adult mammalian brain. in Current Protocols in Neuroscience. 8th edn. (eds. Crawley, J. et al.) 3.10.11-13.10.28 (Wiley, New York, 1997).