Metabolic engineering of novel ketocarotenoid production in carrot plants

Transgenic Research

Volumn 17, Issue 4, 2008, Pages 489-501

  Jayaraj, Jayaraman ^a   Devlin, Robert ^b   Punja, Zamir ^a  

^a SIMON FRASER UNIVERSITY   (Canada)

^b FISHERIES AND OCEANS CANADA   (Canada)

Author keywords

carotene ketolase; Astaxanthin; Biopharming; Carotenoid pathway engineering; Ketocarotenoids; Root pigmentation

Indexed keywords

ACYLTRANSFERASE; ALPHA CAROTENE; ALPHA-CAROTENE; ASTAXANTHIN; BETA CAROTENE HYDROXYLASE; BETA-CAROTENE HYDROXYLASE; CAROTENOID; CRYPTOXANTHIN; ECHINENONE; MIXED FUNCTION OXIDASE; PHOSPHINOTHRICIN N ACETYLTRANSFERASE; PHOSPHINOTHRICIN N-ACETYLTRANSFERASE; RIBULOSEBISPHOSPHATE CARBOXYLASE; UBIQUITIN; XANTHOPHYLL;

ARABIDOPSIS; ARTICLE; CARROT; GENE EXPRESSION REGULATION; GENETIC ENGINEERING; GENETICS; GREEN ALGA; GROWTH, DEVELOPMENT AND AGING; METABOLISM; PEA; PLANT LEAF; PLANT ROOT; PROMOTER REGION; RHIZOBIUM; TRANSGENIC PLANT;

ACETYLTRANSFERASES; ALGAE, GREEN; ARABIDOPSIS; CAROTENOIDS; DAUCUS CAROTA; GENE EXPRESSION REGULATION, PLANT; GENETIC ENGINEERING; MIXED FUNCTION OXYGENASES; PEAS; PLANT LEAVES; PLANT ROOTS; PLANTS, GENETICALLY MODIFIED; PROMOTER REGIONS (GENETICS); RHIZOBIUM; RIBULOSE-BISPHOSPHATE CARBOXYLASE; UBIQUITIN; XANTHOPHYLLS;

AGROBACTERIUM; AGROBACTERIUM RHIZOGENES; ALGAE; ARABIDOPSIS; CYANOBACTERIA; DAUCUS CAROTA; HAEMATOCOCCUS PLUVIALIS; PISUM SATIVUM; RHIZOBIUM;

EID: 46649089939     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-007-9120-0     Document Type: Article

References (49)

1. Botella-Pavía P, Rodríguez-Concepión M (2006) Carotenoid biotechnology in plants for nutritionally improved foods. Physiol Plant 126:369-381
2. Brynt JD, McCord JD, Unlu LK, Erdman JW Jr. (1992) Isolation and partial characterization of α and β-carotene containing carotenoprotein from carrot (Daucus carota L.) root chromoplasts. J Agric Food Chem 40:545-549
3. Camara B (1980) Biosynthesis of keto-carotenoids in Capsicum annuum fruits. FEBS Lett 118:315-318
4. Cunningham FX Jr. (2002) Regulation of carotenoid synthesis and accumulation in plants. Pure Appl Chem 74:1409-1417
5. Cunningham FX Jr., Gantt E (2005) A study in scarlet: enzymes of ketocarotenoid biosynthesis in the flowers of Adonis aestivalis. Plant J 41:478-492
6. D'Ambrosio C, Giorio G, Marino I, Merendino A, Petrozza A, Salfi L, Stigliani AL, Francesco L (2004) Virtually complete conversion of lycopene into β-carotene in fruits of tomato plants transformed with the tomato lycopene β-cyclase (tlcy-b) cDNA. Plant Sci 166:207-214
7. Davidson PA, Hunter CN, Horton P (2002) Overexpression of β-carotene hydroxylase enhances stress tolerance in Arabidopsis. Nature 418:203-206
8. Fischer R, Stoger E, Schillberg S, Christou P, Twyman RM (2004) Plant-based production of biopharmaceuticals. Curr Opin Plant Biol 7:152-158
9. Fraser PD, Bramley P.M. (2004) The biosynthesis and nutritional uses of carotenoids. Prog Lipid Res 43:228-265
10. Fraser PD, Römer S, Shipton CA, Mills PB, Kiano JW, Misawa N, Drake RG, Schuch W, Bramley PM (2002) Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner. Proc Natl Acad Sci USA 99:1092-1097
11. Gerjets T, Sandmann G (2006) Ketocarotenoid formation in transgenic potato. J Exp Bot 57:3639-3645
12. Goodwin TW (1980) The biochemistry of the carotenoids, vol 1, 2nd edn. Chapman and Hall, London
13. Gotz T, Sandmann G, Romer S (2002) Expression of a bacterial carotene hydroxylase gene (crtZ) enhances UV tolerance in tobacco. Plant Mol Biol 50:129-142
14. Guerin M, Huntley ME, Olaizola M (2003) Haematococcus astaxanthin: applications for human health and nutrition. Trends Biotech 21:210-216
15. Hauptmann R, Eschenfeldt WH, English J, Brinkhaus FL (1997) Enhanced carotenoid accumulation in storage organs of genetically engineered plants. US Patent 5618988
16. Higuera-Ciapara I, Félix-Valenzuela L, Goycoolea FM (2006) Astaxanthin: a review of its chemistry and applications. Crit Rev Food Sci Nutr 46:185-196
17. Hirschberg J (2001) Carotenoid biosynthesis in flowering plants. Curr Opin Plant Biol 4:210-218
18. Howitt CA, Pogson BJ (2006) Carotenoid accumulation and function in seeds and non-green tissues. Plant Cell Environ 29:435-445
19. Hussein G, Sankawa U, Goto H, Matsumoto K, Watanabe H (2006) Astaxanthin, a carotenoid with potential in human health and nutrition. J Nat Prod 69:443-449
20. Israel HW, Maples MO, Steward FC (1969) Pigments and plastids in cultures of totipotent carrot cells. Amer J Bot 56:510-917
21. Kajiwara S, Kakizona T, Saito T, Kondo K, Ohtani T, Nagai S, Misawa N (1995) Isolation and functional identification of a novel cDNA for astaxanthin biosynthesis from Haematococcus pluvialis, and astaxanthin synthesis in Escherichia coli. Plant Mol Biol 29:343-352
22. Kim J, DellaPenna D (2006) Defining the primary route for lutein synthesis in plants: the role of Arabidopsis carotenoid β-ring hydroxylase CYP97A3. Proc Natl Acad Sci USA 103:3474-3479
23. Kopsell DA, Kopsell DE (2006) Accumulation and bioavailability of dietary carotenoids in vegetable crops. Trends Plant Sci 11:499-507
24. Lotan T, Hirschberg J (1995) Cloning and expression in Escherichia coli of the gene encoding β-C-4 oxygenase, that converts β-carotene to the ketocarotenoid canthaxanthin in Haematococcus pluvialis. FEBS Lett 364:125-128
25. Mann V, Harker M, Pecker I, Hirschberg J (2000) Metabolic engineering of astaxanthin production in tobacco flowers. Nat Biotech 18:888-892
26. Marx M, Stuparic M, Schieber A, Carle R (2003) Effects of thermal processing on trans-cis-isomerization of β-carotene in carrot juices and carotene-containing preparations. Food Chem 83:609-617
27. Misawa N, Yamano S, Linden H, de Felipe MR, Lucas M, Ikenaga H, Sandmann G (1993) Functional expression of the Erwinia uredovora carotenoid biosynthesis gene crtl in transgenic plants showing an increase of beta-carotene biosynthesis activity and resistance to the bleaching herbicide norflurazon. Plant J 4:833-840
28. Mok MC, Gabelman WH, Skoog F (1976) Carotenoid synthesis in tissue cultures of Daucus carota L. J Amer Soc Hort Sci 101:442-449
29. Morris WL, Ducreux LJM, Fraser PD, Millam S, Taylor MA (2006) Engineering ketocarotenoid biosynthesis in potato tubers. Metab Eng 8:253-263
30. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay of tobacco tissue cultures. Physiol Plant 15:473-479
31. Ralley L, Enfissi EM, Misawa N, Schuch W, Bramley PM, Fraser PD (2004) Metabolic engineering of ketocarotenoid formation in higher plants. Plant J 39:477-486
32. Renstorm B, Berger H, Liaaen-Jensen S (1981) Esterified, optically pure (3S, 3′S)-astaxanthin from flowers of Adonis annua. Biochem Syst Ecol 9:249-250
33. 2 fixation from sugar beet leaves containing calcium oxalate. Photosynthesis Res 4:281-287
34. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
35. Simon PW, Wolff XY, Peterson CE, Kammerlohr DS, Rubatzky VE, Strandberg JO, Bassett MJ, White JM (1989) High carotene mass carrot population. Hort Sci 24:174-175
36. Spiller GA, Dewell A (2003) Safety of an astaxanthin-rich Haematococcus pluvialis algal extract: a randomized clinical trial. J Medicinal Food 6:51-56
37. Stálberg K, Lindgren O, Ek B, Höglund AS (2003) Synthesis of ketocarotenoids in the seed of Arabidopsis thaliana. Plant J 36:771-779
38. Sun Z, Gantt E, Cunningham FX, Jr. (1996) Cloning and functional analysis of the β-carotene hydroxylase of Arabidopsis thaliana. J Biol Chem 271:24349-24352
39. Taylor M, Ramsay G (2005) Carotenoid biosynthesis in plant storage organs: recent advances and prospects for improving plant food quality. Physiol Plant 124:143-151
40. van den Berg H, Faulks R, Fernando Granado H, Hirschberg J, Olmedilla B, Sandmann G, Southon S, Stahl W (2000) The potential for the improvement of carotenoid levels in foods and the likely systemic effects. J Sci Food Agric 80:880-912
41. Vasquez-Caicedo AL, Heller A, Neidhart S, Carle R (2006) Chromoplast morphology and β-carotene accumulation during postharvest ripening of mango cv. 'Tommy Atkins'. J Agric Food Chem 54:5769-5776
42. Visser H, van Ooyen AJJ, Verdoes JC (2003) Metabolic engineering of the astaxanthin-biosynthetic pathway of Xanthophyllomyces dendrohous. FEMS Yeast Res 4:221-231
43. Wally O, Jayaraj J, Punja ZK (2005) Comparative analysis of tissue and wound induced transgene expression levels in carrot (Daucus carota L.) using seven different promoter constructs. Annual Meeting of the American Society of Plant Biologists (Abs)
44. Wally O, Jayaraj J, Punja ZK (2006) Carrot (Daucus carota L.). In: Wang K (ed) Agrobacterium Protocols, vol. II, 2nd edn. Methods in molecular biology book series, Humana Press. Totowa, pp 55-70
45. Ye X, Al-Babili S, Klöti A, Zhang J, Lucca P, Beyer P, Potrykus I (2000) Engineering the provitamin A (beta-carotene) biosynthetic pathway into (carotenoid-free) rice endosperm. Science 287:303-305
46. Yeum KJ, Russell RM (2002) Carotenoid bioavailability and bioconversion. Annu Rev Nutr 22:483-504
47. Yu B, Lydiate DJ, Schafer UA, Hannoufa A (2006) Characterization of a β-carotene hydroxylase of Adonis aestivalis and its expression in Arabidopsis thaliana. Planta, DOI 10.1007/s00425-006-0455-1
48. Yuan YP, Chen F (2000) Purification of trans-astaxanthin from a high yielding astaxanthin ester-producing strain of the microalga Haematococcus pluvialis. Food Chem 68:443-448
49. Zhou JR, Gugger ET, Erdman JW, Jr. (1996) The crystalline form of carotenes and the food matrix in carrot root decrease the relative bioavailability of β- and α-carotene in the ferret model. J Amer College Nutr 15:84-91