Electrochemical detection of thrombin by sandwich approach using antibody and aptamer

Bioelectrochemistry

Volumn 73, Issue 1, 2008, Pages 76-81

  Kang, Yan ^a   Feng, Ke Jun ^a   Chen, Ji Wei ^a   Jiang, Jian Hui ^a   Shen, Guo Li ^a   Yu, Ru Qin ^a  

^a HUNAN UNIVERSITY   (China)

Author keywords

Aptamer; Electrochemical analysis; Nanoparticle; Protein

Indexed keywords

ANTIBODIES; BIOMARKERS; COMPOSITE FILMS; ELECTROCHEMISTRY; MATHEMATICAL MODELS; NANOPARTICLES;

APTAMERS; ELECTROCHEMICAL ANALYSIS; ELECTROCHEMICAL DETECTION; THROMBIN;

ENZYME KINETICS;

ANTIBODY; APTAMER; CARBON; CHITOSAN; GLUTARALDEHYDE; GOLD; METHYLENE BLUE; NANOPARTICLE; THROMBIN;

ARTICLE; CHEMICAL BINDING; CONDUCTANCE; DIFFERENTIAL PULSE VOLTAMMETRY; ELECTROCHEMICAL ANALYSIS; IONIC STRENGTH; PROTEIN ANALYSIS;

ANTIBODIES; APTAMERS, NUCLEOTIDE; CARBON; CHITOSAN; ELECTROCHEMISTRY; ELECTRODES; GLASS; GOLD; HUMANS; MICROSCOPY, ELECTRON, SCANNING; NANOSTRUCTURES; OSMOLAR CONCENTRATION; THROMBIN;

EID: 46549086780     PISSN: 15675394     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bioelechem.2008.04.024     Document Type: Article

References (46)

1. Ellington A.D., and Szostak J.W. In vitro selection of RNA molecules that bind specific ligands. Nature 346 (1990) 818-822
2. Tuerk C., and Gold L. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 249 (1990) 505-510
3. Drolet D.W., Moon-McDermott L., and Romig T.S. An enzymelinked oligonucleotide assay. Nat. Biotechnol. 14 (1996) 1021-1025
4. Kotia R.B., Li L., and McGown L.B. Separation of nontarget compounds by DNA aptamers. Anal. Chem. 72 (2000) 827-831
5. Pavlov V., Shlyahovsky B., and Willner I. Fluorescence detection of DNA by the catalytic activation of an aptamer/thrombin complex. J. Am. Chem. Soc. 127 (2005) 6522-6523
6. Heyduk E., and Heyduk T. Nucleic acid-based fluorescence sensors for detecting proteins. Anal. Chem. 77 (2005) 1147-1156
7. Lin C.X., Katilius E., Liu Y., Zhang J.P., and Yan H. Self-assembled signaling aptamer DNA arrays for protein detection. Angew. Chem. Int. Ed. 45 (2006) 5296-5301
8. Ho H.A., and Leclerc M. Optical sensors based on hybrid aptamer/conjugated polymer complexes. J. Am. Chem. Soc. 126 (2004) 1384-1387
9. Wang X.L., Li F., Su Y.H., Sun X., Li X.B., J Schluesener H., Tang F., and Xu S.Q. Ultrasensitive detection of protein using an aptamer-based exonuclease protection assay. Anal. Chem. 76 (2004) 5605-5610
10. Li J.W., Fang X.H., and Tan W.H. Molecular aptamer beacons for real-time protein recognition. Biochem. Biophys. Res. Commun. 292 (2002) 31-40
11. Heckel A., and Mayer G. Light regulation of aptamer activity: an anti-thrombin aptamer with caged thymidine nucleobases. J. Am. Chem. Soc. 127 (2005) 822-823
12. Pavlov V., Xiao Y., Shlyahovsky B., and Willner I. Aptamer-functionalized Au nanoparticles for the amplified optical detection of thrombin. J. Am. Chem. Soc. 126 (2004) 11768-11769
13. Liss M., Petersen B., Wolf H., and Prohaska E. An aptamer-based quartz crystal protein biosensor. Anal. Chem. 74 (2002) 4488-4495
14. Evans-Nguyen K.M., Fuierer R.R., Fitchett B.D., Tolles L.R., Conboy J.C., and Schoenfisch M.H. Changes in adsorbed fibrinogen upon conversion to fibrin. Langmuir 22 (2006) 5115-5121
15. Bang G.S., Cho S., and Kim B.G. A novel electrochemical detection method for aptamer biosensors. Biosens. Bioelectron. 21 (2005) 863-870
16. Floch F.L., Ho H.A., and Leclerc M. Label-free electrochemical detection of protein based on a ferrocene-bearing cationic polythiophene and aptamer. Anal. Chem. 78 (2006) 4727-4731
17. Lai R.Y., Plaxco K.W., and Heeger A.J. Aptamer-based electrochemical detection of picomolar platelet-derived growth factor directly in blood serum. Anal. Chem. 79 (2007) 229-233
18. Radi A.E., Sa'nchez J.L.A., Baldrich E., and Sullivan C.K.O. Reagentless, reusable, ultrasensitive electrochemical molecular beacon aptasensor. J. Am. Chem. Soc. 128 (2006) 117-124
19. Sa′nchez J.L.A., Baldrich E., Radi A.E.G., Dondapati S., Sa′nchez P.L., Katakis I., and Sullivan C.K.O. Electronic off-on molecular switch for rapid detection of thrombin. Electroanalysis 18 (2006) 1957-1962
20. Xiao Y., Lubin A.A., Heeger A.J., and Plaxco K.W. Label-free electronic detection of thrombin in blood serum by using an aptamer-based sensor. Angew. Chem. 117 (2005) 5592-5595
21. Zhang Y.L., Huang Y., Jiang J.H., Shen G.L., and Yu R.Q. Electrochemical aptasensor based on proximity-dependent surface hybridization assay for single-step, reusable, sensitive protein detection. J. Am. Chem. Soc. 129 (2007) 15448-15449
22. Ikebukuro K., Kiyohara C., and Sode K. Novel electrochemical sensor system for protein using the aptamers in sandwich manner. Biosens. Bioelectron. 20 (2005) 2168-2172
23. Wang H., Yang X.B., Bowick G.C., Herzog N.K., Luxon B.A., Lomas L.O., and Gorenstein D.G. Identification of proteins bound to a thioaptamer probe on a proteomics array. Biochem. Biophys. Res. Commun. 347 (2006) 586-593
24. Baldrich E., Restrepo A., and O¢Sullivan C.K. Aptasensor development: elucidation of critical parameters for optimal aptamer performance. Anal. Chem. 76 (2004) 7053-7063
25. Wang P., Li Y.X., Huang X., and Wang L. Fabrication of layer-by-layer modified multilayer films containing choline and gold nanoparticles and its sensing application for electrochemical determination of dopamine and uric acid. Talanta 73 (2007) 431-437
26. Zhang L.Y., Yuan R., Chai Y.Q., and Li X.L. Investigation of the electrochemical and electrocatalytic behavior of positively charged gold nanoparticle and l-cysteine film on an Au electrode. Anal. Chim. Acta 596 (2007) 99-105
27. Mao X., Jiang J.H., Luo Y., Shen G.L., and Yu R.Q. Copper-enhanced gold nanoparticle tags for electrochemical stripping detection of human IgG. Talanta 73 (2007) 420-424
28. Zhang S., Huang F., Liu B.H., Ding J.J., Xu X., and Kong J.L. A sensitive impedance immunosensor based on functionalized gold nanoparticle-protein composite films for probing apolipoprotein A-I. Talanta 71 (2007) 874-881
29. Du Y., Luo X.L., Xu J.J., and Chen H.Y. A simple method to fabricate a chitosan-gold nanoparticles film and its application in glucose biosensor. Bioelectrochemistry 70 (2007) 342-347
30. Luo X.L., Xu J.J., Zhang Q., Yang G.J., and Chen H.Y. Electrochemically deposited chitosan hydrogel for horseradish peroxidase immobilization through gold nanoparticles self-assembly. Biosens. Bioelectron. 21 (2005) 190-196
31. Xue M.H., Xu Q., Zhou M., and Zhu J.J. In situ immobilization of glucose oxidase in chitosan-gold nanoparticle hybrid film on prussian blue modified electrode for high-sensitivity glucose detection. Electrochem. Commun. 8 (2006) 1468-1474
32. Zhang S.B., Wu Z.S., Guo M.M., Shen G.L., and Yu R.Q. A novel immunoassay strategy based on combination of chitosan and a gold nanoparticle label. Talanta 71 (2007) 1530-1535
33. Li X.Y., Jin L.J., and Mcallister T.A. Chitosan-alginate microcapsules for oral delivery of egg Yolk Immunoglobulin (IgY). J. Agric. Food Chem. 55 (2007) 2911-2917
34. 2+: stability, heat, and hydration. J. Am. Chem. Soc. 123 (2001) 10799-10804
35. Hianik T., Ostatná V., Sonlajtnerova M., and Grman I. Influence of ionic strength, pH and aptamer configuration for binding affinity to thrombin. Bioelectrochemistry 70 (2007) 127-133
36. Hamaguchi N., Ellington A., and Stanton M. Aptamer beacons for the direct detection of proteins. Anal. Biochem. 294 (2001) 126-131
37. Vairamani M., and Gross M.L. G-Quadruplex formation of thrombin-binding aptamer detected by electrospray ionization mass spectrometry. J. Am. Chem. Soc. 125 (2003) 42-43
38. Bock L.C., Griffin L.C., Latham J.A., Vermass E.H., and Toole J.J. Selection of single-stranded DNA molecules that bind and inhibit human thrombin. Nature 355 (1992) 564-566
39. Tuite E., and Norden B. Sequence-specific interactions of methylene blue with polynucleotides and DNA: a spectroscopic study. J. Am. Chem. Soc. 116 (1994) 7548-7556
40. Tuite E., and Kelly J.M. The interaction of methylene blue, azure B, and thionine with DNA: formation of complexes with polynucleotides and mononucleotides as model systems. Biopolymers 35 (1995) 419-433
41. Kara P., Kerman K., Ozkan D., Meric B., Erdem A., Ozkan Z., and Ozsoz M. Electrochemical genosensor for the detection of interaction between methylene blue and DNA. Electrochem. Commun., 4 (2002) 705-709
42. Lin X.H., Wu P., Chen W., Zhang Y.F., and Xia X.H. Electrochemical DNA biosensor for the detection of short DNA species of Chronic Myelogenous Leukemia by using methylene blue. Talanta 72 (2007) 468-471
43. Rohs R., Sklenar H., and Lavery R. Methylene blue binding to DNA with alternating GC base sequence: A modeling study. J. Am. Chem. Soc. 122 (2000) 2860-2866
44. Meric B., Kerman K., Ozkan D., Kara P., Erensoy S., Akarca U.S., Mascini M., and Ozsoz M. Electrochemical DNA biosensor for the detection of TT and Hepatitis B virus from PCR amplified real samples by using methylene blue. Talanta 56 (2002) 837-846
45. Qi Z.M., Matsuda N., Takatsu A., and Kato K. In situ investigation of coadsorption of myoglobin and methylene blue to hydrophilic glass by broadband time-resolved optical waveguide spectroscopy. Langmuir 20 (2004) 778-784
46. Kelley S.O., and Barton J.K. Electrochemistry of methylene blue bound to a DNA-modified electrode. Bioconjug. Chem. 8 (1997) 31-37