Fluorescence detection of DNA by the catalytic activation of an aptamer/thrombin complex

Journal of the American Chemical Society

Volumn 127, Issue 18, 2005, Pages 6522-6523

  Pavlov, Valeri ^a   Shlyahovsky, Bella ^a   Willner, Itamar ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

APTAMER; DNA; THROMBIN;

ARTICLE; CATALYSIS; DNA DETERMINATION; FLUORESCENCE ANALYSIS; HYBRIDIZATION; HYDROLYSIS;

CATALYSIS; DNA; DNA, SINGLE-STRANDED; ENZYME ACTIVATION; FLUORESCENT DYES; MODELS, MOLECULAR; OLIGONUCLEOTIDES; RHODAMINES; SPECTROMETRY, FLUORESCENCE; THROMBIN;

EID: 18644363565     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja050678k     Document Type: Article

References (26)

1. Luzi, E.; Minunni, M.; Tombelli, S.; Mascini, M. Trends Anal. Chem. 2003, 11, 810-818.
2. (a) Fukusho, S.; Furusawa, H.; Okahata, Y. Chem. Commun. 2002, 88-89.
3. (b) Kawakami, J.; Hirofumi, I.; Yukie, Y.; Sugimoto, N. J. Inorg. Biochem. 2000, 82, 197-206.
4. (c) Kleinjung, F.; Klussman, S.; Erdmann, V. A.; Scheller, F. W.; Furste, J. P.; Bier, F. F. Anal. Chem. 1998, 70, 328-331.
5. (d) Potyrailo, R. A.; Conrad, R. C.; Ellington, A. D.; Hieftje, G. M. Anal. Chem. 1998, 70, 3419-3425.
6. (e) Myoyong, L.; Walt, D. R. Anal. Biochem. 2000, 282, 142-146.
7. (f) Heyduk, E.; Heyduk, T. Anal. Chem. 2005, 77, 1147-1156.
8. (a) Bock, L. C.; Griffin, L. C.; Latham, J. A.; Vermaas, E. H.; Toole, J. J. Nature 1992, 355, 564-566.
9. (b) Macaya, R. F.; Waldron, J. A.; Beutel, B. A.; Gao, H. T.; Joesten, M. E.; Yang, M. H.; Patel, R.; Bertelsen, A. H.; Cook, A. F. Biochemistry 1995, 34, 4478-4492.
10. (c) Padmanabhan, K.; Tulinsky, A. Acta Crystallogr. 1990, D52, 272-282.
11. (a) Kelly, J. A.; Feigon, J.; Yeates, T. O. J. Mol. Biol. 1996, 256, 417-422.
12. (b) Tasset, D. M.; Kubik, M. F.; Steiner, W. J. Mol. Biol. 1997, 272, 688-698.
13. (c) Wu, Q.; Tsiang, M.; Sadler, J. E. J. Biol. Chem. 1992, 267, 24408-24412.
14. (a) Ho, H. A.; Leclerc, M. J. Am. Chem. Soc. 2004, 126, 1384-1387.
15. (b) Pavlov, V.; Xiao, Y.; Shlyahovsky, B.; Willner, I. J. Am. Chem. Soc. 2004, 126, 11768-11769.
16. (a) Shin, J.-S.; Pierce, N. A. J. Am. Chem. Soc. 2004, 126, 10834-10835.
17. (b) Alberti, P.; Mergny, J.-L. Proc. Natl. Acad. Sci. U.S.A. 2003, 100, 1569-1573.
18. (c) Yin, P.; Yan, H.; Daniell, X. G.; Turberfield, A. J.; Rief, J. H. Angew. Chem., Int. Ed. 2004, 43, 4906-4911.
19. (a) Xiao, Y.; Pavlov, V.; Niazov, T.; Dishon, A.; Kotler, M.; Willner, I. J. Am. Chem. Soc. 2004, 126, 7430-7431.
20. (b) Dittmer, W. U.; Reuter, A.; Simmel, F. C. Angew. Chem., Int. Ed. 2004, 43, 3550-3553.
21. (c) Yurke, B.; Turberfield, A. J.; Mills, A. P., Jr.; Simmel, F. C.; Neumann, J. L. Nature 2000, 406, 605-608.
22. Smith, P. K.; Krohn, R. I.; Hermanson, G. T.; Mallia, A. K.; Gartner, F. H.; Provenzano, M. D.; Fujimoto, E. K.; Goeke, N. M.; Olson, B. J.; Klenk, D. C. Anal. Biochem. 1985, 150, 76-85.
23. A calibration curve for the OliGreen fluorescence responses at variable concentrations of 1 was extracted. The fluorescence intensity of 1-modified Th was then monitored to determine the total concentration of 1. Knowing the protein concentration, we extracted the average loading of Th.
24. Zweig, S. E.; Meyer, B. G.; Sharma, S.; Min, C.; Krakower, J. M.; Shohet, S. B. Biomed. Instrum. Technol. 1996, 30, 245-256.
25. Saghatelian, A.; Guckian, K. M.; Thayer, D. A.; Ghadiri, M. R. J. Am. Chem. Soc. 2003, 125, 344-345.
26. 2, 5% glycerol (w/v).