Design, expression, and purification of a Flaviviridae polymerase using a high-throughput approach to facilitate crystal structure determination

Protein Science

Volumn 13, Issue 10, 2004, Pages 2685-2692

  Choi, Kyung H ^a   Groarke, James M ^b,c   Young, Dorothy C ^b   Rossmann, Michael G ^a   Pevear, Daniel C ^b   Kuhn, Richard J ^a   Smith, Janet L ^a  

^a PURDUE UNIVERSITY   (United States)

^b ViroPharma Incorporated   (United States)

^c NOVARTIS INSTITUTES FOR BIOMEDICAL RESEARCH   (United States)

Author keywords

Bovine viral diarrhea virus; Flaviviridae; Hepatitis C virus; High throughput; Limited proteolysis; NS5B; RNA dependent RNA polymerase

Indexed keywords

PROTEIN; RNA DIRECTED RNA POLYMERASE; TRYPSIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CRYSTAL STRUCTURE; ENZYME PURIFICATION; ESCHERICHIA COLI; FLAVIVIRUS; HEPATITIS C VIRUS; HIGH THROUGHPUT SCREENING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; SEQUENCE HOMOLOGY; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; CATTLE; CLONING, MOLECULAR; CRYSTALLIZATION; CRYSTALLOGRAPHY; DIARRHEA VIRUSES, BOVINE VIRAL; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; RNA REPLICASE; TRYPSIN; VIRAL PROTEINS;

BOVINAE; BOVINE VIRAL DIARRHEA VIRUS 1; ESCHERICHIA COLI; FLAVIVIRIDAE; FLAVIVIRUS; HEPATITIS C VIRUS; RNA VIRUSES;

EID: 4644272643     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.04872204     Document Type: Article

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