The HBSP gene is expressed during HBV replication, and its coded BH3-containing spliced viral protein induces apoptosis in HepG2 cells

Biochemical and Biophysical Research Communications

Volumn 351, Issue 1, 2006, Pages 64-70

  Lu, Yi Wei ^a   Tan, Tuan Lin ^a   Chan, Vincent ^b   Chen, Wei Ning ^b  

^a NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

^b Nanyang Technological University   (Singapore)

Author keywords

Apoptosis; BH3; HBSP; HBV replication

Indexed keywords

BH3 PROTEIN; CASPASE; HEPATITIS B SPLICED PROTEIN; UNCLASSIFIED DRUG; VIRUS PROTEIN;

AMINO ACID SEQUENCE; ANIMAL CELL; APOPTOSIS; ARTICLE; CELL DEATH; CONTROLLED STUDY; CYTOPATHOGENIC EFFECT; FLOW CYTOMETRY; FUNCTIONAL PROTEOMICS; GENETIC TRANSFECTION; HEPATITIS B; HEPATITIS B VIRUS; HUMAN; HUMAN CELL; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; VIRUS GENE; VIRUS GENOME; VIRUS INFECTIVITY; VIRUS REPLICATION; VIRUS VIRULENCE;

APOPTOSIS; BH3 INTERACTING DOMAIN DEATH AGONIST PROTEIN; CELL LINE; DNA, RECOMBINANT; GENE EXPRESSION REGULATION, VIRAL; HEPATITIS B VIRUS; HEPATOCYTES; HUMANS; VIRAL PROTEINS; VIRUS REPLICATION;

HEPATITIS B VIRUS; MAMMALIA;

EID: 33750347280     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2006.10.002     Document Type: Article

References (21)

1. Lau J.Y., Xie X., Lai M.M., and Wu P.C. Apoptosis and viral hepatitis. Semin. Liver Dis. 18 (1998) 169-176
2. Adams J.M., and Cory S. The Bcl-2 protein family: arbiters of cell survival. Science 281 (1998) 1322-1326
3. Baell J.B., and Huang D.C. Prospects for targeting the Bcl-2 family of proteins to develop novel cytotoxic drugs. Biochem. Pharmacol. 64 (2002) 851-863
4. Brechot C. Pathogenesis of hepatitis B virus-related hepatocellular carcinoma: old and new paradigms. Gastroenterology 127 Suppl. 1 (2004) S56-S61
5. Chen G.G., Lai P.B., Chan P.K., Chak E.C., Yip J.H., Ho R.L., Leung B.C., and Lau W.Y. Decreased expression of Bid in human heaptocellular carcinoma is related to hepatitis B virus X protein. Eur. J. Cancer 37 (2001) 1695-1702
6. Ehrmann J., Galuszkova D., Ehrmann J., Krc I., Jezdinska V., Vojtesek B., Murray P.G., and Kolao Z. Apoptosis-related proteins, Bcl-2, Bax, Fas, Fas-L and PCNA in liver biopsies of patients with chronic hepatitis B virus infection. Pathol. Oncol. Res. 6 (2000) 130-135
7. Teodoro J.G., and Branton P.E. Regulation of apoptosis by viral gene products. J. Virol. 71 (1997) 1739-1746
8. Kelekar A., and Thomson C.B. Bcl-2-family proteins: the role of the BH3 domain in apoptosis. Trends Cell Biol. 8 (1998) 324-329
9. Chen W.N., Oon C.J., and Toh I. Altered antigenicities of hepatitis B virus surface antigen carrying mutations outside the common "a" determinant. Am. J. Gastroenterol. 95 (2000) 1098-1099
10. Soussan P., Garreau F., Zylberberg H., Ferray C., Brechot C., and Kremsdorf D. In vivo expression of a new hepatitis B virus protein encoded by a spliced RNA. J. Clin. Invest. 105 (2000) 55-60
11. Oon C.J., Chen W.N., Goh K.T., Mesenas S., Ng H.S., Chiang G., Tan C., Koh S., Teng S.W., Toh I., Moh M.C., Goo K.S., Tan K., Leong A.L., and Tan G.S. Molecular characterization of hepatitis B virus surface antigen mutants in Singapore patients with hepatocellular carcinoma and hepatitis B virus carriers negative for HBsAg but positive for anti-HBs and anti-HBc. J. Gastroenterol. Hepatol. 17 Suppl. (2002) S491-S496
12. Lee A.T., Ren J., Wong E.T., Ban K.H., Lee L.A., and Lee C.G. The hepatitis B virus X protein sensitizes HepG2 cells to UV light-induced DNA damage. J. Biol. Chem. 280 (2005) 33525-33535
13. Lu Y.W., and Chen W.N. Human hepatitis B virus X protein induces apoptosis in HepG2 cells: role of BH3 domain. Biochem. Biophys. Res. Commun. 338 (2005) 1551-1556
14. Huang H.L., Jeng K.S., Hu C.P., Tsai C.H., Lo S.J., and Chang C. Identification and characterization of a structural protein of hepatitis B virus: a polymerase and surface fusion protein encoded by a spliced RNA. Virology 275 (2000) 398-410
15. Razvi E.S., and Welsh R.M. Apoptosis in viral infections. Adv. Virus. Res. 45 (1995) 1-60
16. Roulston A., Marcellus R.C., and Branton P.E. Viruses and apoptosis. Annu. Rev. Microbiol. 53 (1999) 577-628
17. Soussan P., Tuveri R., Nalpas B., Garreau F., Zavala F., Masson A., Pol S., Brechot C., and Kremsdorf D. The expression of hepatitis B spliced protein (HBSP) encoded by a spliced hepatitis B virus RNA is associated with viral replication and liver fibrosis. J. Hepatol. 38 (2003) 343-348
18. Boatright K.M., and Salvesen G.S. Mechanisms of caspase activation. Curr. Opin. Cell Biol. 15 (2003) 725-731
19. Sattler M., Liang H., Nettesheim D., Meadows R.P., Harlan J.E., Eberstadt M., Yoon H.S., Shuker S.B., Chang B., Minn A.J., Thompson C.B., and Fesik S.W. Structure of Bcl-xL-Bak peptide complex: recognition between regulators of apoptosis. Science 275 (1997) 983-986
20. Su T.S., Lai C.J., Huang J.L., Lin L.H., Yauk Y.K., Chang C.M., Lo S.J., and Han S.H. Hepatitis B virus transcript produced by RNA splicing. J. Virol. 63 (1989) 4011-4018
21. Gunther S., Sommer G., Iwanska A., and Will H. A new class of defective hepatitis B virus genomes with an internal poly(dA) sequence. Virology 24 (1997) 363-371