A spliced hepadnavirus RNA that is essential for virus replication

EMBO Journal

Volumn 15, Issue 10, 1996, Pages 2565-2574

  Obert, Sabine ^a   Zachmann Brand, Beate ^a   Deindl, Elisabeth ^a,b   Tucker, Will ^a,c   Bartenschlager, Ralf ^a,d   Schaller, Heinz ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b MAX PLANCK INSTITUTE FOR HEART AND LUNG RESEARCH   (Germany)

^c AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

^d JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

Duck hepatitis B virus; Large surface protein; RNA splicing; Subgenomic RNAs

Indexed keywords

GLYCINE CLEAVAGE SYSTEM; MEMBRANE PROTEIN; MESSENGER RNA; RNA PRECURSOR; TRANSFER RNA; VIRUS RNA; L PROTEIN, HEPATITIS B VIRUS; VIRUS ENVELOPE PROTEIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DNA VIRUS; DUCK; HEPATITIS B VIRUS; LIVER CELL; NONHUMAN; PRIORITY JOURNAL; RNA SPLICING; VIRUS REPLICATION; ANIMAL; GENETIC TRANSFECTION; GENETICS; INTRON; LIVER; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; VIROLOGY; VIRUS INFECTION;

ANIMALS; BASE SEQUENCE; DUCKS; HEPADNAVIRIDAE INFECTIONS; HEPATITIS B VIRUS, DUCK; INTRONS; LIVER; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RNA SPLICING; RNA, VIRAL; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, NUCLEIC ACID; TRANSFECTION; VIRAL ENVELOPE PROTEINS; VIRUS REPLICATION;

EID: 0030010478     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1002/j.1460-2075.1996.tb00614.x     Document Type: Article

References (46)

1. Ausubel,F.M., Brent,R., Kingston,R.E., Moore,D.D., Seidman,J.G., Smith,J.A. and Struhl,K. (1987) In Current Protocols in Molecular Biology. John Wiley & Sons, New York, p. 4.2.1.
2. Bartenschlager,R. (1990) Strukturelle und funktionelle Charakterisierung des P-Proteins der Hepatitis B Viren. Ph.D. Thesis, University of Heidelberg.
3. Bartenschlager,R., Kuhn,C. and Schaller,H. (1992) Expression of the P-protein of the human hepatitis B virus in a vaccinia virus system and detection of the nucleocapsid-associated P-gene product by radiolabelling at newly introduced phosphorylation sites. Nucleic Acids Res., 20, 195-202.
4. Bartos,H. (1995) Zur Rolle der Polymerase der Hepatitis B Viren in den frühen Schritten der Replikation. Ph.D. Thesis, University of Heidelberg.
5. Büscher,M., Reiser,W., Will,H, and Schaller,H. (1985) Transcripts and the putative RNA pregenome of duck hepatitis B virus: implications for reverse transcription. Cell. 40, 717-724.
6. Calvert,J. and Summers,J. (1994) Two regions of an avian hepadnavirus RNA pregenome are required in cis for encapsidation. J. Virol., 68, 2084-2090.
7. Chomczynski,P. and Sacchi,N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem., 162, 156-159.
8. Condreay,L.D., Aldrich,C.E., Coates,L., Mason,W.S. and Wu,T.T. (1990) Efficient duck hepatitis B virus production by an avian liver tumor cell line. J. Virol., 64, 3249-3258.
9. Condreay,L.D., Wu,T.T., Aldrich,C.E., Delaney,M.A., Summers,J., Seeger,C. and Mason,W.S. (1992) Replication of DHBV genomes with mutations at the sites of intiation of minus-and plus-strand DNA synthesis. Virology, 188, 208-216.
10. Deindl,E. (1994) Gespleißte Transkripte des Enten Hepatitis B Virus: Charakterisierung und Mutationsanalyse. Ph.D. Thesis, University of Heidelberg.
11. Fernholz,D., Galle,P.R., Stemler,M., Brunetto,M, Bonino,F. and Will,H. (1993) Infectious hepatitis B virus variant defective in pre-S2 protein expression in a chronic carrier. Virology, 194, 137-148.
12. Ganem,D. and Varmus,H.E. (1987) The molecular biology of the hepatitis B viruses. Anna. Rev. Biochem., 56, 651-693.
13. Grgacic,E.V.L. and Anderson,D.A. (1994) The large surface protein of duck hepatitis B virus is phosphorylated in the preS domain. J. Virol., 68, 7344-7350.
14. Heermann,K.H., Goldmann,U., Schwartz,W., Seyffarth,T. Baumgarten,H. and Gerlich,W.H. (1984) Large surface proteins of hepatitis B virus containing the preS sequence. J. Virol., 52, 396-402.
15. Howard,C.R. (1991) Classification and nomenclature of viruses. Family Hepadnaviridae. Arch. Virol., 2 (Suppl.), 111-116.
16. Huang,M. and Summers,J. (1994) pet, a small sequence distal to the pregenome cap site, is required for expression of the duck hepatitis B virus pregenome. J. Virol. 68, 1564-1572.
17. Ishikawa,T. and Ganem,D. (1995) The pre-S domain of the large viral envelope protein determines host range in avian hepatitis B viruses. Proc. Natl Acad. Sci. USA. 92, 6259-6263.
18. Junker-Niepmann,M., Bartenschlager,R. and Schaller,H. (1990) A short cis-acting sequence is required for hepatitis B virus pregenome encapsidation and sufficient for packaging of foreign RNA. EMBO J., 9, 3389-3396.
19. Kiss-Laszlo,Z., Blanc,S. and Hohn,T. (1995) Splicing of cauliflower mosaic virus 35S RNA is essential for viral infectivity. EMBO J., 14, 3552-3562.
20. Klingmuller,U. and Schaller,H. (1993) Hepadnavirus infection requires interaction between the viral pre-S domain and a specific hepatocellular receptor. J. Virol., 67, 7414-7422.
21. Knaus,T. and Nassal,M. (1993) The encapsidation signal on the hepatitis B virus RNA pregenome forms a stem-loop structure that is critical for its function. Nucleic Acids Res., 21, 3967-3975.
22. Kunkel,T.A., Roberts,J.D. and Zakour,R.A. (1987) Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol., 154, 367-382.
23. Lenhoff,R.J. and Summers,J. (1994) Coordinate regulation of replication and virus assembly by the large envelope protein of an avian hepadnavirus. J. Virol. 68, 4565-4571.
24. Lien,J.M., Petcu,D.J., Aldrich,C.E. and Mason,W.S. (1987) Initiation and termination of duck hepatitis B virus DNA synthesis during virus maturation. J. Virol., 61, 3832-3840.
25. Mandart,E., Kay,A. and Galibert,F. (1984) Nucleotide sequence of a cloned duck hepatitis B virus genome: comparison with woodchuck and human hepatitis B virus sequences. J. Virol., 49, 782-792.
26. Moore,M.J., Query,C.C. and Sharp,P.A. (1993) Splicing of precursors to mRNA by the spliceosome. In Gesteland,R.F. and Atkins,J.F. (eds), The RNA World. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, NY, pp. 303-357.
27. Nassal,M. (1996) Hepatitis B virus morphogenesis. Curr. Topics Microbiol. Immunol., in press.
28. Nassal,M. and Schaller,H. (1993) Hepatitis B virus replication. Trends Microbiol., 1, 221-228.
29. Neumann,M., Harrison,J., Saltarelli,M., Haziyannis,E., Erfle,V., Felber,B.K. and Pavlakis,G.N. (1994) Splicing variability in HIV type I revealed by quantitative RNA polymerase chain reaction. AIDS Res. Hum. Retroviruses, 10, 1531-1542.
30. Promega Protocols and Application Guide. 2nd edn. (1991) Promega Corp., Madison, WI, pp. 58-59.
31. Pugh,J.C. and Summers,J.W. (1989) Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide. Virology. 172, 564-572.
32. Ogston,C.W. and Razman,D.G. (1992) Spliced RNA of woodchuck hepatitis virus. Virology, 189, 245-252.
33. Rigg,R.J. and Schaller,H. (1992) Duck hepatitis B virus injection of hepatocytes is not dependent on low pH. J. Virol., 66, 2829-2836.
34. Sambrook,J., Fritsch,E.F. and Maniatis,T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
35. Schaller,H. and Fischer,M. (1991) Transcription control of hepadnaviral gene expression. Curr. Topics Microbiol. Immunol., 168, 181-192.
36. Schlicht,H.J., Bartenschlager,R. and Schaller,H. (1989) The duck hepatitis B virus core protein contains a highly phosphorylated C terminus that is essential for replication but not for RNA packaging. J. Virol., 63, 2995-3000.
37. Schneider,R., Fernholz,D., Wildner,G. and Will,H. (1991) Mechanism. kinetics, and role of duck hepatitis B virus e-antigen expression in vivo. Virology. 182, 503-512.
38. Sprengel,R., Kaleta,E.F. and Will,H. (1988) Isolation and characterization of a hepatitis B virus endemic in herons. J. Virol., 62, 3832-3839.
39. Stoltzfus,C.M. (1988) Synthesis and processing of avian sarcoma retrovirus RNA. Adv. Virus Res., 35, 1-38.
40. Su,T.-S., Lui,W.-Y., Lin,L.-H., Han,S.-H. and Peng,F.-K. (1989) Analysis of hepatitis B virus transcripts in infected human livers. Hepatology, 9, 180-185.
41. Summers,J., and Mason,W.S. (1982) Replication of the genome of a hepatitis B-like virus by reverse transcription of an RNA intermediate. Cell. 29, 403-415.
42. Summers,J., Smith,P.M. and Horwich,A.L. (1990) Hepadnavirus envelope proteins regulate covalently closed circular DNA amplification. J. Virol., 64, 2819-2824.
43. Terré,S., Petit,M.-A. and Brechot,C. (1991) Defective hepatitis B virus particles are generated by packaging and reverse transcription of spliced viral RNAs in viva. J. Virol., 65, 5539-5543.
44. Tuttleman,J.S., Pugh,J.C. and Summers,J.W. (1986) In vitro experimental infection of primary duck hepatocyte cultures with duck hepatitis B virus. J. Virol., 58, 17-25.
45. Wang,G.H. and Seeger,C. (1993) Novel mechanism for reverse transcription in hepatitis B viruses. J. Virol., 67, 6507-6512.
46. Wu,H.-L., Chen,P.-J., Tu,S.-J., Lin,M.-H., Lai,M.-Y. and Chen,D.-S. (1991) Characterization and genetic analysis of alternatively spliced transcripts of hepatitis B virus in infected human liver tissues and transfected HepG2 cells. J. Virol., 65, 1680-1686.