The flexibility of the non-conservative region at the C terminus of D-hydantoinase from Pseudomonas putida YZ-26 is extremely limited

Applied Biochemistry and Biotechnology

Volumn 144, Issue 3, 2008, Pages 237-247

  Zhang, Xue Yao ^a   Niu, Li Xi ^a   Shi, Ya Wei ^a   Yuan, Jing Ming ^a  

^a SHANXI UNIVERSITY   (China)

Author keywords

C terminal deletion and substitution; D Hydantoinase; Properties; Structural modeling; Subunit dissociation

Indexed keywords

ACIDS; AMINES; AMINO ACIDS; CHROMATOGRAPHIC ANALYSIS; ENZYMES; FOOD ADDITIVES; METAL IONS; METALS; MONOMERS; ORGANIC ACIDS; PORT TERMINALS; SIZE EXCLUSION CHROMATOGRAPHY; THREE DIMENSIONAL;

C-TERMINAL DELETION AND SUBSTITUTION; C-TERMINUS; D-HYDANTOINASE; DIVALENT METAL IONS; ENZYMATIC ACTIVITIES; FOUR AMINO ACIDS; HYDANTOINASE; IN-VITRO; IN-VIVO; PROPERTIES; PSEUDOMONAS PUTIDA; STRUCTURAL MODELING; SUBUNIT DISSOCIATION; TERMINAL RESIDUES; WILD-TYPE ENZYME;

ENZYME ACTIVITY;

DEXTRO HYDANTOINASE; DIHYDROPYRIMIDINASE; AMIDASE; MANGANESE; MUTANT PROTEIN; ZINC;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVITY; GEL PERMEATION CHROMATOGRAPHY; GENE DELETION; NONHUMAN; PSEUDOMONAS PUTIDA; THERMOSTABILITY; CHEMICAL STRUCTURE; CHEMISTRY; DRUG ANTAGONISM; ENZYME STABILITY; ENZYMOLOGY; GENETICS; METABOLISM; MUTATION; PH; PROTEIN TERTIARY STRUCTURE; TEMPERATURE;

PSEUDOMONAS PUTIDA;

AMIDOHYDROLASES; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; MANGANESE; MODELS, MOLECULAR; MUTANT PROTEINS; MUTATION; PROTEIN STRUCTURE, TERTIARY; PSEUDOMONAS PUTIDA; TEMPERATURE; ZINC;

EID: 46049098827     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12010-007-8004-3     Document Type: Article

References (21)

1. Y. W. Shi L. X. Niu X. Feng J. M. Yuan 2006 Purification, enzymatic properties of a recombinant D-hydantoinase and its dissociation by zinc ion World Journal of Microbiology and Biotechnology 22 675 680
2. Y. W. Shi L. X. Zhao L. X. Niu J. M. Yuan 2005 Gene Sequence, soluble expression and homologous comparison of a D-Hydantoinase from Pseudomonas putida YZ-26 Chemical Research in Chinese University 21 552 557
3. L. X. Niu X. Y. Zhang Y. W. Shi J. M. Yuan 2007 Subunit dissociation and stability alteration of D-Hydantoinase deleted at the terminal amino acid residue Biotechnology Letters 29 303 308
4. O. May M. Siemann M. G. Siemann C. Syldatk 1998 Catalytic and structural function of zinc for the hydantoinase from Anthrobacter aurescens DSM 3745 Journal of Molecular Catalysis B, Enzymatic 4 211 218
5. J. Ogawa S. Shimizu 1999 Microbial enzymes: new industrial applications from traditional screening methods Trends in Biotechnology 17 13 20
6. C. Y. Huang Y. P. Chao Y. S. Yang 2003 Purification of industrial hydantoinase in one chromatographic step without affinity tag Protein Expression and Purification 30 134 139
7. Z. Xu Y. Q. Liu Y. L. Yang W. H. Jiang E. Arnold J. P. Ding 2003 Crystal Structure of D-Hydantoinase from Burkholderia pickettii at a resolution of 2.7 angstroms: Insights into the molecular basis of enzyme thermostability Journal of Bacteriology 185 4038 4049
8. J. Abendroth K. Niefind D. Schomburg 2002 X-ray structure of a Dihydropyrimidinase from Thermus sp. at 1.3 Å resolution Journal of Molecular Biology 320 143 156
9. I. Abe T. Abe W. W. Lou T. Masuola H. Noguchi 2007 Site-directed mutagenesis of conserved aromatic residues in rat squalene epoxidase Biochemical and Biophysical Research Communications 352 259 263
10. D. Villadsen T. H. Nielsen 2001 N-terminal truncation affects the kinetics and structure of fructose-6-phosphate 2-kinase/fructose-2,6- bisphosphatase from Arabidopsis thaliana Biochemical Journal 359 591 597
11. Y. H. Cheon H. S. Park S. C. Lee D. E. Lee H. S. Kim 2003 Structure-based mutational analysis of the active site residues of d-hydantoinase Journal of Molecular Catalysis. B, Enzymatic 26 217 222
12. G. J. Kim H. S. Kim 1998 C-Terminal Regions of D-Hydantoinases are nonessential for catalysis, but affect the oligomeric structure Biochemical and Biophysical Research Communications 243 96 100
13. U. Laemmli 1970 Cleavage of structural proteins during the assembly of bacteriophage T4 Nature 227 680 685
14. T. Schwede J. Kopp N. Guex M. C. Peitsch 2003 SWISS-MODEL: an automated protein homology-modeling server Nucleic Acids Research 31 3381 3385
15. J. A. Gerlt F. M. Raushel 2003 Evolution of function in (β/α)8-barrel enzymes Current Opinion in Chemical Biology 7 252 264
16. B. Lohkamp B. Andersen J. Piskur D. Dobritzsch 2006 The crystal structures of dihydropyrimidinases reaffirm the close relationship between cyclic amidohydrolases and explain their substrate specificity Journal of Biological Chemistry 281 13762 13776
17. Y. H. Cheon H. S. Kim K. H. Han J. Abendroth K. Niefind D. Schomburg 2002 Crystal structure of D-Hydantoinase from Bacillus stearothermophilus: Insight into the stereochemistry of enantioselectivity Biochemist 41 9410 9417
18. K. V. Radha Kishan R. M. Vohra K. Ganesan V. Agrawal V. M. Sharma R. Sharma 2005 Molecular structure of D-Hydantoinase from Bacillus sp. AR9: Evidence for Mercury inhibition Journal of Molecular Biology 347 95 105
19. S. Skoulakis J. M. Goodfellow 2003 The pH-dependent stability of wild-type and mutant Transthyretin Oligomers Biophysical Journal 84 2795 2804
20. Y. Z. Mei B. F. He P. K. Ouyang 2005 Progress in study on thermostability and substrate specificity of hydantoinase Chinese Journal of Bioprocess Engineering 3 24 28
21. L. Holm C. Sander 1997 An evolutionary treasure: Unification of a broad set of Amidohydrolases related to urease Proteins: Structure, Function and Genetics 28 72 82