Mucins and their O-Glycans from human bronchial epithelial cell cultures

American Journal of Physiology - Lung Cellular and Molecular Physiology

Volumn 287, Issue 4 31-4, 2004, Pages

  Holmén, Jessica M ^a   Karlsson, Niclas G ^b   Abdullah, Lubna H ^c   Randell, Scott H ^c,d   Sheehan, John K ^c   Hansson, Gunnar C ^a   Davis, C William ^c,d  

^a UNIVERSITY OF GOTHENBURG   (Sweden)

^b Proteome Systems Ltd   (Australia)

^c UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^d UNIVERSITY OF NORTH CAROLINA   (United States)

Author keywords

Cystic fibrosis; Mucus overproduction; Obstructive airway disease

Indexed keywords

AGAROSE; GLYCAN; GLYCAN DERIVATIVE; MESSENGER RNA; MUCIN; MUCIN 5B; OLIGOSACCHARIDE;

ADULT; ARTICLE; BRONCHUS MUCOSA; CELL CULTURE; CHEMICAL STRUCTURE; CLINICAL ARTICLE; CONTROLLED STUDY; CORRELATION ANALYSIS; CYSTIC FIBROSIS; ENVIRONMENTAL FACTOR; EPITHELIUM CELL; FEMALE; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; MALE; MASS SPECTROMETRY; PHENOTYPE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; WESTERN BLOTTING;

ADOLESCENT; ADULT; BRONCHI; BRONCHIOLITIS OBLITERANS; CARBOHYDRATE SEQUENCE; CELLS, CULTURED; ELECTROSTATICS; FEMALE; GENOTYPE; HUMANS; KINETICS; MALE; MIDDLE AGED; MOLECULAR SEQUENCE DATA; MUCINS; OLIGOSACCHARIDES; POLYSACCHARIDES; RESPIRATORY MUCOSA;

EID: 4544354750     PISSN: 10400605     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajplung.00108.2004     Document Type: Article

References (51)

1. 2+ activation of mucin secretion in airway goblet cells. Am J Physiol Lung Cell Mol Physiol 273: L201-L210, 1997.
2. Abdullah LH, Davis SW, Burch L, Yamauchi M, Randell SH, Nettesheim P, and Davis CW. P2u purinoceptor regulation of mucin secretion in SPOCI cells, a goblet cell line from the airways. Biochem J 316: 943-951, 1996.
3. Axelsson MA, Karlsson NG, Steel DM, Ouwendijk J, Nilsson T, and Hansson GC. Neutralization of pH in the Golgi apparatus causes redistribution of glycosyltransferases and changes in the O-glycosylation of mucins. Glycobiology 11: 633-644, 2001.
4. Barasch J, Kiss B, Prince A, Saiman L, Gruenert D, and Al Awqati Q. Defective acidification of intracellular organelles in cystic fibrosis. Nature 352: 70-73, 1991.
5. Bernacki SH, Nelson AL, Abdullah L, Sheehan JK, Harris A, William DC, and Randell SH. Mucin gene expression during differentiation of human airway epithelia in vitro. MUC4 and MUC5B are strongly induced. Am J Respir Cell Mol Biol 20: 595-604, 1999.
6. Breg J, van Halbeek H, Vliegenthart JF, Klein A, Lamblin G, and Roussel P. Primary structure of neutral oligosaccharides derived from respiratory-mucus glycoproteins of a patient suffering from bronchiectasis, determined by combination of 500-MHz 1H-NMR spectroscopy and quantitative sugar analysis. II. Structure of 19 oligosaccharides having the GlcNAc beta(1-3)GalNAc-ol core (type 3) or theGlcNAc beta(1-3)[GlcNAc beta(1-6)]GalNAc-ol core (type 4). Eur J Biochem 171: 643-654, 1988.
7. Brockhausen I, Vavasseur F, and Yang X. Biosynthesis of mucin type O-glycans: lack of correlation between glycosyltransferase and sulfotransferase activities and CFTR expression. Glycoconj J 18: 685-697, 2001.
8. Bry L, Falk PG, Midtvedt T, and Gordon JI. A model of host-microbial interactions in an open mammalian ecosystem. Science 273: 1380-1383, 1996.
9. Chandy G, Grabe M, Moore HP, and Machen TE. Proton leak and CFTR in regulation of Golgi pH in respiratory epithelial cells. Am J Physiol Cell Physiol 281: C908-C921, 2001.
10. Cheng PW, Boat TF, Cranfill K, Yankaskas JR, and Boucher RC. Increased sulfation of glycoconjugates by cultured nasal epithelial cells from patients with cystic fibrosis. J Clin Invest 84: 68-72, 1989.
11. Chirgwin JM, Przbyla AE, MacDonald RJ, and Rutter WJ. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18: 5294-5299, 1979.
12. Chung KF, Cytokines in chronic obstructive pulmonary disease. Eur Respir J Suppl 34: 50s-59s, 2001.
13. Davies JR, Herrmann A, Russell W, Svitacheva N, Wickstrom C, and Carlstedt I. Respiratory tract mucins: structure and expression patterns. Novartis Found Symp 248: 76-88, 2002.
14. Davies JR, Svitacheva N, Lannefors L, Kornfalt R, and Carlstedt I. Identification of MUC5B, MUC5AC and small amounts of MUC2 mucins in cystic fibrosis airway secretions. Biochem J 344: 321-330, 1999.
15. Davril M, Degroote S, Humbert P, Galabert C, Dumur V, Lafitte JJ, Lamblin G, and Roussel P, The sialylation of bronchial mucins secreted by patients suffering from cystic fibrosis or from chronic bronchitis is related to the severity of airway infection. Glycobiology 9: 311-32, 1999.
16. Desseyn JL, Buisine MP, Porchet N, Aubert JP, and Laine A. Genomic organization of the human mucin gene MUC5B. cDNA and genomic sequences upstream of the large central exon. J Biol Chem 273: 30157-30164, 1998.
17. Dosanjh A, Lencer W, Brown D, Ausiello DA, and Stow JL. Heterologous expression of delta F508 CFTR results in decreased sialylation of membrane glycoconjugates. Am J Physiol Cell Physiol 266: C360-C366, 1994.
18. Engelhardt JF, Yankaskas JR, Ernst SA, Yang Y, Marino CR, Boucher RC, Cohn JA, and Wilson JM. Submucosal glands are the predominant site of CFTR expression in the human bronchus. Nat Genet 2: 240-248, 1992.
19. Escande F, Aubert JP, Porchet N, and Buisine MP. Human mucin gene MUC5AC: organization of its 5'-region and central repetitive region. Biochem J 358: 763-772, 2001.
20. Gibson GA, Hill WG, and Weisz OA. Evidence against the acidification hypothesis in cystic fibrosis. Am J Physiol Cell Physiol 279: C1088-C1099, 2000.
21. Gray TE, Guzman K, Davis CW, Abdullah LH, and Nettesheim P. Mucociliary differentiation of serially passaged normal human tracheobronchial epithelial cells. Am J Respir Cell Mol Biol 14: 104-112, 1996.
22. Holmen JM, Olson FJ, Karlsson H, and Hansson GC, Two glycosylation alterations of mouse intestinal mucins due to infection caused by the parasite Nippostrongylus brasiliensis. Glycoconj J 19: 67-75, 2002.
23. Karlsson NG and Hansson GC. Analysis of monosaccharide composition of mucin oligosaccharide alditols by high-performance anion-exchange chromatography. Anal Biochem 224: 538-541, 1995.
24. Karlsson NG, Herrmann A, Karlsson H, Johansson ME, Carlstedt I, and Hansson GC. The glycosylation of rat intestinal Muc2 mucin varies between rat strains and the small and large intestine. A study of O-linked oligosaccharides by a mass spectrometric approach. J Biol Chem 272: 27025-27034, 1997.
25. Karlsson NG, Karlsson H, and Hansson GC. Strategy for the investigation of O-linked oligosaccharides from mucins based on the separation into neutral, sialic acid- and sulfate-containing species. Glycoconj J 12: 69-76, 1995.
26. Kirkham S, Sheehan JK, Knight D, Richardson PS, and Thornton DJ. Heterogeneity of airways mucus: variations in the amounts and glycoforms of the major oligomeric mucins MUC5AC and MUC5B. Biochem J 361: 537-546, 2002.
27. Knowles MR and Boucher RC. Mucus clearance as a primary innate defense mechanism for mammalian airways. J Clin Invest 109: 571-577, 2002.
28. Kuver R, Klinkspoor JH, Osborne WR, and Lee SP. Mucous granule exocytosis and CFTR expression in gallbladder epithelium. Glycobiology 10: 149-157, 2000.
29. Lamblin G, Degroote S, Perini JM, Delmotte P, Scharfman A, Davril M, Lo-Guidice JM, Houdret N, Dumur V, Klein A, and Roussel P. Human airway mucin glycosylation: a combinatory of carbohydrate determinants which vary in cystic fibrosis. Glycoconj J 18: 661-684, 2001.
30. Lethem MI, Dowell ML, Van Scott M, Yankaskas JR, Egan T, Boucher RC, and Davis CW. Nucleofide regulation of goblet cells in human airway epithelial explants: normal exocytosis in cystic fibrosis. Am J Respir Cell Mol Biol 9: 315-322, 1993.
31. Li JD, Dohrman AF, Gallup M, Miyata S, Gum JR, Kim YS, Nadel JA, Prince A, and Basbaum CB. Transcriptional activation of mucin by Pseudomonas aeruginosa lipopolysaccharide in the pathogenesis of cystic fibrosis lung disease. Proc Natl Acad Sci USA 94: 967-972, 1997.
32. Mall M, Kreda SM, Mengos A, Jensen TJ, Hirtz S, Seydewitz HH, Yankaskas J, Kunzelmann K, Riordan JR, and Boucher RC. The deltaF508 mutation results in loss of cystic fibrosis transmembrane conductance regulator function and mature protein in native human colon. Gastroenterology 126: 32-41, 2004.
33. Matsui H, Grubb BR, Tarran R, Randell SH, Gatzy JT, Davis CW, and Boucher RC. Evidence for periciliary liquid layer depletion, not abnormal ion composition, in the pathogenesis of cystic fibrosis airways disease. Cell 95: 1005-1015, 1998.
34. Matsui H, Randell SH, Peretti SW, Davis CW, and Boucher RC. Coordinated clearance of periciliary liquid and mucus from airway surfaces. J Clin Invest 102: 1125-1131, 1998.
35. Moniaux N, Escande F, Porchet N, Aubert JP, and Batra SK. Structural organization and classification of the human mucin genes. Front Biosci 6: D1192-D1206, 2001.
36. Morelle W, Sutton-Smith M, Morris HR, Davril M, Roussel P, and Dell A. FAB-MS characterization of sialyl Lewis x determinants on polylactosamine chains of human airway mucins secreted by patients suffering from cystic fibrosis or chronic bronchitis. Glycoconj J 18: 699-708, 2001.
37. Nielsen PA, Bennett EP, Wandall HH, Therkildsen MH, Hannibal J, and Clausen H. Identification of a major human high molecular weight salivary mucin (MGI) as tracheobronchial mucin MUC5B. Glycobiology 7: 413-419, 1997.
38. Pilewski JM and Frizzell RA. Role of CFTR in airway disease. Physiol Rev 79: S215-S255, 1999.
39. Puchelle E, Gaillard D, Ploton D, Hinnrasky J, Fuchey C, Boutterin MC, Jacquot J, Dreyer D, Pavirani A, and Dalemans W. Differential localization of the cystic fibrosis transmembrane conductance regulator in normal and cystic fibrosis airway epithelium. Am J Respir Cell Mol Biol 7: 485-491, 1992.
40. Randell SH, Walstad L, Schwab UE, Grubb BR, and Yankaskas JR. Isolation and culture of airway epithelial cells from chronically infected human lungs. In Vitro Cell Dev Biol Anim 37: 480-489, 2001.
41. Reid CJ, Gould S, and Harris A, Developmental expression of mucin genes in the human respiratory tract. Am J Respir Cell Mol Biol 17: 592-598, 1997.
42. Rhim AD, Stoykova L, Glick MC, and Scanlin TF. Terminal glycosylation in cystic fibrosis (CF): a review emphasizing the airway epithelial cell. Glycoconj J 18: 649-659, 2001.
43. Rogers DF. Airway goblet cells: responsive and adaptable front-line defenders. Eur Respir J 7: 1690-1706, 1994.
44. Sangadala S, Bhat UR, and Mendicino J. Structures of sulfated oligosaccharides in human trachea mucin glycoproteins. Mol Cell Biochem 126: 37-47, 1993.
45. Scanlin TF and Glick MC. Terminal glycosylation in cystic fibrosis. Biochim Biophys Acta 1455: 241-253, 1999.
46. Schulz BL, Packer NH, and Karlsson NG. Small-scale analysis of O-linked oligosaccharides from glycoproteins and mucins separated by gel electrophoresis. Anal Chem 74: 6088-6097, 2002.
47. Sheehan JK, Oates K, and Carlstedt I. Electron microscopy of cervical, gastric and bronchial mucus glycoproteins. Biochem J 239: 147-153, 1986.
48. Thomsson KA, Hinojosa-Kurtzberg M, Axelsson KA, Domino SE, Lowe JB, Gendler SJ, and Hansson GC. Intestinal mucins from cystic fibrosis mice show increased fucosylation due to an induced Fucalphal-2 glycosyltransferase. Biochem J 367: 609-616, 2002.
49. Thornton DJ, Carlstedt I, and Sheehan JK. Identification of glycoproteins on nitrocellulose membranes and gels. Mol Biotechnol 5: 171-176, 1996.
50. Van Halbeek H, Breg J, Vliegenthart JF, Klein A, Lamblin G, and Roussel P. Isolation and structural characterization of low-molecularmass monosialyl oligosaccharides derived from respiratory-mucus glycoproteins of a patient suffering from bronchiectasis. Eur J Biochem 177: 443-460, 1988.
51. Zhang Y, Doranz B, Yankaskas JR, and Engelhardt JF. Genotypic analysis of respiratory mucous sulfation defects in cystic fibrosis. J Clin Invest 96: 2997-3004, 1995.