Mucin gene expression during differentiation of human airway epithelia in vitro MUC4 and MUC5b are strongly induced

American Journal of Respiratory Cell and Molecular Biology

Volumn 20, Issue 4, 1999, Pages 595-604

  Bernacki, Susan H ^b   Nelson, Andrew L ^b   Abdullah, Lubna ^b   Sheehan, John K ^b   Harris, Ann ^b   Davis, C William ^b   Randell, Scott H ^a  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; MUCIN; MUCIN 4; MUCIN 5B; PRIMER DNA; RETINOIC ACID;

ARTICLE; BIOSYNTHESIS; CELL CULTURE; CELL DIFFERENTIATION; CHROMOSOME 11; CYTOLOGY; DRUG EFFECT; EPITHELIUM CELL; GENE EXPRESSION REGULATION; GENETICS; HUMAN; LUNG; METABOLISM; MULTIGENE FAMILY; NOSE MUCOSA; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

CELL DIFFERENTIATION; CELLS, CULTURED; CHROMOSOMES, HUMAN, PAIR 11; DNA PRIMERS; EPITHELIAL CELLS; GENE EXPRESSION REGULATION; HUMANS; LUNG; MUCINS; MULTIGENE FAMILY; NASAL MUCOSA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TRETINOIN;

EID: 0033109380     PISSN: 10441549     EISSN: None     Source Type: Journal    
DOI: 10.1165/ajrcmb.20.4.3442     Document Type: Article

References (56)

1. Phillips, G. J., S. L. James, and M. I. Lethem. 1997. Rheological properties and hydration of airway mucus. In Airway Mucus: Basic Mechanisms and Clinical Perspectives. D. F. Rogers and M. I. Lethem, editors. Birkhauser Verlag, Basel, Switzerland. 117-147.
2. Wells, U. M., and P. S. Richardson. 1997. Mucus hypersecretion and its role in the airway obstruction of asthma and chronic obstructive pulmonary disease. In Airway Mucus: Basic Mechanisms and Clinical Perspectives. D. F. Rogers and M. I. Lethem, editors. Birkhauser Verlag, Basel, Switzerland. 275-300.
3. Pigny, P., V. Guyonnet-Duperat, A. S. Hill, W. S. Pratt, S. Galiegue-Zouitina, M. C. d'Hooge, A. Laine, I. Van-Seuningen, P. Degand, J. R. Gum, Y. S. Kim, D. M. Swallow, J. P. Aubert, and N. Porchet. 1996. Human mucin genes assigned to 11p15.5: identification and organization of a cluster of genes. Genomics 38:340-352.
4. Gendler, S. J., C. A. Lancaster, J. Taylor-Papadimitriou, T. Duhig, T. Peat, and J. Burchell. 1990. Molecular cloning and expression of a human tumor-associated polymorphic epithelial mucin. J. Biol. Chem. 265:15286-15293.
5. Lan, M. S., S. K. Batra, W.-N. Qi, R. S. Metzgar, and M. A. Hollingsworth. 1990. Cloning and sequencing of a human pancreatic tumor mucin cDNA. J. Biol. Chem. 265:15294-15299.
6. Gum, J. R., Jr., J. W. Hicks, N. W. Toribara, B. Siddiki, and Y. S. Kim. 1994. Molecular cloning of human intestinal mucin (MUC2) cDNA. Identification of the amino terminus and overall sequence similarity to prepro-von Willebrand factor. J. Biol. Chem. 269:2440-2446.
7. Li, J. D., A. F. Dohrman, M. Gallup, S. Miyata, J. R. Gum, Y. S. Kim, J. A. Nadel, A. Prince, and C. B. Basbaum. 1997. Transcriptional activation of mucin by Pseudomonas aeruginosa lipopolysaccharide in the pathogenesis of cystic fibrosis lung disease. Proc. Nat. Acad. Sci. USA 94:967-972.
8. Gum, J. R., J. W. Hicks, and Y. S. Kim. 1997. Identification and characterization of the MUC2 (human intestinal mucin) gene 5′-flanking region: promoter activity in cultured cells. Biochem. J. 325:259-267.
9. Bobek, L. A., J. Liu, S. N. Sait, T. B. Shows, Y. A. Bobek, and M. J. Levine. 1996. Structure and chromosomal localization of the human salivary mucin gene, MUC7. Genomics 31:277-282.
10. Bobek, L. A., H. Tsai, A. R. Biesbrock, and M. J. Levine. 1993. Molecular cloning, sequence, and specificity of expression of the gene encoding the low molecular weight human salivary mucin (MUC7). J. Biol. Chem. 268: 20563-20569.
11. Porchet, N., V. C. Nguyen, J. Dufosse, J. P. Audie, V. Guyonnet-Duperat, M. S. Gross, C. Denis, P. Degand, A. Bernheim, and J. P. Aubert. 1991. Molecular cloning and chromosomal localization of a novel human tracheo-bronchial mucin cDNA containing tandemly repeated sequences of 48 base pairs. Biochem. Biophys. Res. Commun. 175:414-422.
12. Meerzaman, D., P. Charles, E. Daskal, M. H. Polymeropoulos, B. M. Martin, and M. C. Rose. 1994. Cloning and analysis of cDNA encoding a major airway glycoprotein, human tracheobronchial mucin (MUC5). J. Biol. Chem. 269:12932-12939.
13. Aubert, J.-P., N. Porchet, M. Crepin, M. Duterque-Coquillaud, G. Vergnes, M. Mazzuca, B. Debuire, D. Petitprez, and P. Degand. 1991. Evidence for different human tracheobronchial mucin peptides deduced from nucleotide cDNA sequences. Am. J. Respir. Cell Mol. Biol. 5:178-185.
14. Dufosse, J., N. Porchel, J.-P. Audie, V. Guyonnet-Duperat, A. Laine, I. Van-Seuningen, S. Marrakchi, P. Degand, and J.-P. Aubert. 1993. Degenerate 87-base-pair tandem repeats create hydrophilic/hydrophobic alternating domains in human mucin peptides mapped to 11p15. Biochem. J. 293:329-337.
15. Shankar, V., P. Pichan, R. L. Eddy, Jr., V. Tonk, N. Nowak, S. N. Sait, T. B. Shows, R. E. Schultz, G. Gotway, R. C. Elkins, M. S. Gilmore, and G. P. Sachdev. 1997. Chromosomal localization of a human mucin gene (MUC8) and cloning of the cDNA corresponding to the carboxy terminus. Am. J. Respir. Cell Mol. Biol. 16:232-241.
16. Sharma, P., L. Dudus, P. A. Nielsen, H. Clausen, J. R. Yankaskas, M. A. Hollingsworth, and J. F. Englehardt. 1998. MUC5B and MUC7 are differentially expressed in mucous and serous cells of submucosal glands in human bronchial airways. Am. J. Respir. Cell Mol. Biol. 19:30-37.
17. Chambers, J. A., M. A. Hollingsworth, A. E. Trezise, and A. Harris. 1994. Developmental expression of mucin genes MUC1 and MUC2. J. Cell Sci. 107:413-424.
18. Reid, C. J., S. Gould, and A. Harris. 1997. Developmental expression of mucin genes in the human respiratory tract. Am. J. Respir. Cell Mol. Biol. 17:592-598.
19. Nguyen, P. L., G. A. Niehans, D. L. Cherwitz, Y. S. Kim, and S. B. Ho. 1996. Membrane-bound (MUC1) and secretory (MUC2, MUC3, and MUC4) mucin gene expression in human lung cancer. Tumour Biol. 17:176-192.
20. De Bolos, C., M. Garrido, and F. X. Real. 1995. MUC6 apomucin shows a distinct normal tissue distribution that correlates with Lewis antigen expression in the human stomach [comment]. Gastroenterology 109:723-734.
21. Hovenberg, H. W., J. R. Davies, and I. Carlstedt. 1995. Human tracheal mucins - is MUC5 more prominent in the epithelial surface than in the submucosa? Biochem. Soc. Trans. 23:534S. (Abstr.)
22. Hovenberg, H. W., J. R. Davies, A. Herrmann, C. J. Linden, and I. Carlstedt. 1996. MUC5AC, but not MUC2, is a prominent mucin in respiratory secretions. Glycoconj. J. 13:839-847.
23. Thornton, D. J., I. Carlstedt, M. Howard, P. L. Devine, M. R. Price, and J. K. Sheehan. 1996. Respiratory mucins: identification of core proteins and glycoforms. Biochem. J. 316:967-975.
24. Thornton, D. J., M. Howard, N. Khan, and J. K. Sheehan. 1997. Identification of two glycoforms of the MUC5B mucin in human respiratory mucus. Evidence for a cysteine-rich sequence repeated within the molecule. J. Biol. Chem. 272:9561-9566.
25. Yoon, J. H., T. Gray, K. Guzman, J. S. Koo, and P. Nettesheim. 1997. Regulation of the secretory phenotype of human airway epithelium by retinoic acid, triiodothyronine, and extracellular matrix. Am. J. Respir. Cell Mol. Biol. 16:724-731.
26. Finkbeiner, W. E., S. D. Carrier, and C. E. Teresi. 1993. Reverse transcription-polymerase chain reaction (RT-PCR) phenotypic analysis of cell cultures of human tracheal epithelium, tracheobronchial glands, and lung carcinomas. Am. J. Respir. Cell Mol. Biol. 9:547-556.
27. Kai, H., K. Yoshitake, A. Hisatsune, T. Kido, Y. Isohama, K. Takahama, and T. Miyata. 1996. Dexamethasone suppresses mucus production and MUC-2 and MUC-5AC gene expression by NCI-II292 cells. Am. J. Physiol. 271:L484-L488.
28. Li, D., D. Wang, S. Majumdar, B. Jany, S. R. Durham, J. Cottrell, N. Caplen, D. M. Geddes, E. W. Alton, and P. K. Jeffery. 1997. Localization and up-regulation of mucin (MUC2) gene expression in human nasal biopsies of patients with cystic fibrosis. J. Pathol. 181:305-310.
29. Seregni, E., C. Botti, C. Lombardo, A. Cantoni, A. Bogni, I. Cataldo, and E. Bombardieri. 1996. Pattern of mucin gene expression in normal and neoplastic lung tissues. Anticancer Res. 16:2209-2213.
30. Inatomi, T., S. Spurr-Michaud, A. S. Tisdale, Q. Zhan, S. T. Feldman, and I. K. Gipson. 1996. Expression of secretory mucin genes by human conjunctival epithelia. Invest. Ophthalmol. Vis. Sci. 37:1684-1692.
31. Gray, T. E., K. Guzman, C. W. Davis, L. H. Abdullah, and P. Nettesheim. 1996. Mucociliary differentiation of serially passaged normal human tracheobronchial epithelial cells. Am. J. Respir. Cell Mol. Biol. 14:104-112.
32. Wu, R., J. Yankaskas, E. Cheng, M. R. Knowles, and R. Boucher. 1985. Growth and differentiation of human nasal epithelial cells in culture: serum-free, hormone-supplemented medium and proteoglycan synthesis. Am. Rev. Respir. Dis. 132:311-320.
33. Yankaskas, J. R., C. U. Cotton, M. R. Knowles, J. T. Gatzy, and R. C. Boucher. 1985. Culture of human nasal epithelial cells on collagen matrix supports: a comparison of bioelectric properties of normal and cystic fibrosis epithelia. Am. Rev. Respir. Dis. 132:1281-1287.
34. Willumsen, N. J., C. W. Davis, and R. C. Boucher. 1989. Intracellular Cl-activity and cellular Cl-pathways in cultured human airway epithelium. Am. J. Physiol. 256:C1033-C1044.
35. Lechner, J. F., and M. A. LaVeck. 1985. A serum-free method for culturing normal human bronchial epithelial cells at clonal density. J. Tissue Cult. Meth. 9:43-48.
36. Kaartinen, L., P. Nettesheim, K. B. Adler, and S. H. Randell. 1993. Rat tracheal epithelial cell differentiation in vitro. In Vitro Cell. Dev. Biol. Anim. 29A:481-492.
37. Sims, D. E., and M. M. Horne. 1997. Heterogeneity of the composition and thickness of tracheal mucus in rats. Am. J. Physiol. 273:L1036-L1041.
38. Voynow, J. A., and M. C. Rose. 1994. Quantitation of mucin mRNA in respiratory and intestinal epithelial cells. Am. J. Respir. Cell Mol. Biol. 11: 742-750.
39. Guzman, K., T. E. Gray, J. H. Yoon, and P. Nettesheim. 1996. Quantitation of mucin RNA by PCR reveals induction of both MUC2 and MUC5AC mRNA levels by retinoids. Am. J. Physiol. 271:L1023-L1028.
40. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1997. Current protocols in molecular biology. In Current Protocols. V. Chanda, editor. John Wiley & Sons, Inc., New York.
41. Porchet, N., P. Pigny, M. P. Buisine, V. Debailleul, P. Degand, A. Laine, and J. P. Aubert. 1995. Human mucin genes: genomic organization and expression of MUC4, MUC5AC and MUC5B. Biochem. Soc. Trans. 23:800-805.
42. Haendler, B., and E. Hofer. 1990. Characterization of the human cyclophilin gene and of related processed pseudogenes. Eur. J. Biochem. 190:477-482.
43. Debailleul, V., A. Laine, G. Huet, P. Mathon, M. C. d'Hooghe, J. P. Aubert, and N. Porchet. 1998. Human mucin genes MUC2, MUC3, MUC4, MUC5AC, MUC5B, and MUC6 express stable and extremely large mRNAs and exhibit a variable length polymorphism: an improved method to analyze large mRNAs. J. Biol. Chem. 273:881-890.
44. Gendler, S. J., and A. P. Spicer. 1995. Epithelial mucin genes. Annu. Rev. Physiol. 57:607-634.
45. Shankar, V., M. S. Gilmore, R. C. Elkins, and G. P. Sachdev. 1994. A novel human airway mucin cDNA encodes a protein with unique tandem-repeat organization. Biochem. J. 300:295-298.
46. Van Klinken, B. J., T. C. Van Dijken, E. Oussoren, H. A. Buller, J. Dekker, and A. W. Einerhand. 1997. Molecular cloning of human MUC3 cDNA reveals a novel 59 amino acid tandem repeat region. Biochem. Biophys. Res. Commun. 238:143-148.
47. Apostolopoulos, V., P. X. Xing, and I. F. McKenzie. 1995. Anti-peptide monoclonal antibodies to intestinal mucin 3. J. Gastroenterol. Hepatol. 10:555-561.
48. Toribara, N. W., A. M. Roberton, S. B. Ho, W. L. Kuo, E. Gum, J. W. Hicks, J. R. Gum, Jr., J. C. Byrd, B. Siddiki, and Y. S. Kim. 1993. Human gastric mucin: identification of a unique species by expression cloning. J. Biol. Chem. 268:5879-5885.
49. Audie, J. P., A. Janin, N. Porchet, M. C. Copin, B. Gosselin, and J. P. Aubert. 1993. Expression of human mucin genes in respiratory, digestive, and reproductive tracts ascertained by in situ hybridization. J. Histochem. Cytochem. 41:1479-1485.
50. Lo-Guidice, J. M., M. D. Merten, G. Lamblin, N. Porchet, M. C. Houvenaghel, C. Figarella, P. Roussel, and J. M. Perini. 1997. Mucins secreted by a transformed cell line derived from human tracheal gland cells. Biochem. J. 326:431-437.
51. Basbaum, C., and B. Jany. 1990. Plasticity in the airway epithelium. Am. J. Physiol. 259:L38-L46.
52. Sommerhoff, C. P., and W. E. Finkbeiner. 1990. Human tracheobronchial submucosal gland cells in culture. Am. J. Respir. Cell Mol. Biol. 2:41-50.
53. Jetten, A. M. 1991. Growth and differentiation factors in tracheobronchial epithelium. Am. J. Physiol. 260:L361-L373.
54. Fox, M. F., F. Lahbib, W. Pratt, J. Attwood, J. Gum, Y. Kim, and D. M. Swallow. 1992. Regional localization of the intestinal mucin gene MUC3 to chromosome 7q22. Ann. Hum. Genet. 56:281-287.
55. Gum, J. R., J. W. Hicks, D. M. Swallow, R. L. Lagace, J. C. Byrd, D. T. Lamport, B. Siddiki, and Y. S. Kim. 1990. Molecular cloning of cDNAs derived from a novel human intestinal mucin gene. Biochem. Biophys. Res. Commun. 171:407-415.
56. Gross, M. S., V. Guyonnet-Duperat, N. Porchet, A. Bernheim, J. P. Aubert, and V. C. Nguyen. 1992. Mucin 4 (MUC4) gene: regional assignment (3q29) and RFLP analysis. Ann. Genet. 35:21-26.