The anticoagulant thrombin mutant W215A/E217A has a collapsed primary specificity pocket

Journal of Biological Chemistry

Volumn 279, Issue 38, 2004, Pages 39824-39828

  Pineda, Agustin O ^a   Chen, Zhi Wei ^a   Caccia, Sonia ^a,b   Cantwell, Angelene M ^a,c   Savvides, Savvas N ^a,d   Waksman, Gabriel ^a,e   Mathews, F Scott ^a   Di Cera, Enrico ^a,f  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF MILAN   (Italy)

^c UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

^d GHENT UNIVERSITY   (Belgium)

^e UNIVERSITY OF LONDON   (United Kingdom)

^f WASHINGTON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CRYSTALLIZATION; HYDROGEN BONDS; IONS; PROTEINS; SUBSTRATES;

ABROGATION; ANTICOAGULANT PROTEINS; MUTANTS; PROTEASE;

CATALYSIS;

ANTICOAGULANT AGENT; CARBOXYLIC ACID; MUTANT PROTEIN; THROMBIN;

AMINO ACID SEQUENCE; ANTICOAGULATION; ARTICLE; CATALYSIS; COMPLEX FORMATION; CRYSTAL STRUCTURE; CRYSTALLIZATION; ENZYME SUBSTRATE; HYDROGEN BOND; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; STRUCTURE ANALYSIS; X RAY ANALYSIS;

ANTICOAGULANTS; BINDING SITES; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; HUMANS; MUTATION; PROTEIN STRUCTURE, TERTIARY; SUBSTRATE SPECIFICITY; THROMBIN;

STAPHYLOCOCCUS PHAGE 187;

EID: 4544312894     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M407272200     Document Type: Article

References (26)

1. Griffin, J. H. (1995) Nature 378, 337-338
2. Di Cera, E. (2003) Chest 124, (suppl.) 11-17
3. Esmon, C. T. (2003) Chest 124, (suppl.) 26-32
4. Wu, Q. Y., Sheehan, J. P., Tsiang, M., Lentz, S. R., Birktoft, J. J., and Sadler, J. E. (1991) Proc. Natl Acad. Sci. U. S. A. 88, 6775-6779
5. Le Bonniec, B. F., and Esmon, C. T. (1991) Proc. Natl. Acad. Sci. U. S. A. 88, 7371-7375
6. Gibbs, C. S., Contre, S. E., Tsiang, M., Li, W. X., Jain, A. K., Dunn, K. E., Law, V. S., Mao, C. T., Matsumura, S. Y., Mejza, S. J., Paborsky, L. R., and Leung, L. L. K. (1995) Nature 378, 413-416L. L. K.L. L.
7. Dang, Q. D., Guinto, E. R., and di Cera, E. (1997) Nat. Biotechnol. 15, 146-149
8. Cantwell, A. M., and Di Cera, E. (2000) J. Biol. Chem. 275, 39827-39830
9. Gruber, A., Cantwell, A. M., Di Cera, E., and Hanson, S. R. (2002) J. Biol. Chem. 277, 27581-27584
10. Otwinowski, Z. (1993) in Proceedings of the CCP4 Study Weekend: Data Collection and Processing (Sawyers, L., Isaacs, N., and Bailey, S., eds) pp. 56-62, SERC Daresbury Laboratory, Warrington, U. K.
11. Otwinowski, Z., and Minor, W. (1997) Methods Enzymol. 276, 307-326
12. Bode, W., Turk, D., and Karshikov, A. (1992) Protein Sci. 1, 426-471
13. Brunger, A. T., Adams, P. D., Clore, G. M., DeLano, W. L., Gros, P., Grosse-Kunstleve, R. W., Jiang, J. S., Kuszewski, J., Nilges, M., Pannu, N. S., Read, R. J., Rice, L. M., Simonson, T., and Warren, G. L. (1998) Acta Crystallogr. Sect. D Biol. Crystallogr. 54, 905-921
14. Jones, T. A., Zou, J. Y., Cowan, S. W., and Kjeldgaard. (1991) Acta Crystallogr. Sect. A 47, 110-119
15. Pineda, A. O., Carrell, C. J., Bush, L. A., Prasad, S., Caccia, S., Chen, Z.-W., Mathews, F. S., and Di Cera, E. (2004) J. Biol. Chem. 279, 31842-31853
16. Kleywegt, G. J. (1999) Acta Crystallogr. Sect. D Biol. Crystallogr. 55, 1878-1884
17. Nayal, M., and Di Cera, E. (1996) J. Mol. Biol. 256, 228-234
18. Prasad, S., Wright, K. J., Roy, D. B., Bush, L. A., Cantwell, A. M., and Di Cera, E. (2003) Proc. Natl. Acad. Sci. U. S. A. 100, 13785-13790
19. Banfield, D. K., and MacGillivray, R. T. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 2779-2783
20. Vindigni, A., White, C. E., Komives, E. A., and Di Cera, E. (1997) Biochemistry 36, 6674-6681
21. Fuentes-Prior, P., Iwanaga, Y., Huber, R., Pagila, R., Rumennik, G., Seto, M., Morser, J., Light, D. R., and Bode, W. (2000) Nature 404, 518-525
22. Guinto, E. R., Caccia, S., Rose, T., Futterer, K., Waksman, G., and Di Cera, E. (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 1852-1857
23. Pineda, A. O., Savvides, S. N., Waksman, G., and Di Cera, E. (2002) J. Biol. Chem. 277, 40177-40180
24. Carter, W. J., Myles, T., Gibbs, C. S., Leung, L. L., and Huntington, J. A. (2004) J. Biol. Chem. 279, 26387-26394
25. Krem, M. M., and Di Cera, E. (2003) Biophys. Chem. 100, 315-323
26. Morris, A. L., MacArthur, M. W., Hutchinson, E. G., and Thornton, J. M. (1992) Proteins 12, 345-364